13C and 15N partitioning among shoots, roots, and soil in Brassica napus genotypes varying in seed oil content potential

Fei, Houman; McVetty, P. B. E.; Vessey, J. Kevin

doi:10.1016/j.bcab.2013.02.001

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…The study of Fei et al (2013) indicated an inverse relationship between seed oil content potential on the one hand, and biomass, C, and N accumulation on the other. Hence, the partition of mobilized C and N from vegetative biomass to seeds seems to determine the realized seed oil content much more.…”

Section: Defining the Ideotype Of A Nitrogen-efficient Rapeseedmentioning

confidence: 96%

Nitrogen use efficiency in rapeseed. A review

Bouchet

Laperche

Bissuel-Bélaygue

et al. 2016

Agron. Sustain. Dev.

175

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Mineral nitrogen fertilization has improved crop yield over the last century but has also caused air and water pollution. Reduction of nitrogen inputs and maintaining high yields are therefore essential to ensure a more sustainable agriculture. Improving the nitrogen use efficiency (NUE) of crops is therefore needed. Rapeseed, Brassica napus, depends on nitrogen fertilization due to its low NUE, with the ratio of plant nitrogen content to nitrogen supplied often not exceeding 60 %. Here, we review the major phenotypic traits associated with NUE in B. napus, with special emphasis on winter oilseed rape. We discuss the genetic diversity available and potential breeding strategies. The major points are the following: (1) rapeseed seed yield elaboration is complex, with overlapping phases of nitrogen uptake and remobilization during the crop cycle; (2) traits related to nitrogen uptake, such as root length and the amount of nitrogen absorbed after flowering, and traits related to nitrogen remobilization, such as the "staygreen" phenotype, have been identified as possible levers to improve NUE in rapeseed; (3) a substantial body of studies investigating the genetic control of NUE traits have already published and potential candidate genes identified; and (4) rapeseed genetic diversity may be enriched by exploiting interpopulation genetic variation and the closely related gene pools of Brassica rapa and Brassica oleracea.

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Section: Defining the Ideotype Of A Nitrogen-efficient Rapeseedmentioning

confidence: 96%

Nitrogen use efficiency in rapeseed. A review

Bouchet

Laperche

Bissuel-Bélaygue

et al. 2016

Agron. Sustain. Dev.

175

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“…Plants of the two mutant genetic lines and their WT parents were labeled with 13 CO 2 at growth stage 6.5 in a custom-designed labeling chamber (Fei et al 2013) During labeling, the PPFD was 300 mol·m −2 ·s −1 , the temperature ranged approximately between 24 and 28°C, the relative humidity ranged between 90% and 95%, and the atmospheric CO 2 concentration was monitored by a CO 2 gas analyzer (LiCor-820; LI-COR Biosciences, Lincoln, Nebraska, USA).…”

Section: Labelingmentioning

confidence: 99%

Carbon partitioning in tissues of a gain-of-function mutant (MYB75/PAP1-D) and a loss-of-function mutant (myb75-1) inArabidopsis thaliana

FeiHouman

EllisBrian

Kevin

2014

Botany

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The role of myeloblastosis (MYB) transcription factors in regulation of carbon flow among the various products of the phenylpropanoid pathway is still unclear. Carbon partitioning in Arabidopsis thaliana (L.) Heynh. was studied in a gain-of-function mutant (MYB75/PAP1-D) and a loss-of-function mutant (myb75-1). Determination of anthocyanins and total phenolics levels in leaf, peduncle, and root tissues of these mutants and their wild-type (WT) parental lines was assessed, as well as 13 C partitioning between "extractives" (predominantly cytoplasm) and "nonextractives" (predominantly cell wall) of these tissues. The amounts of anthocyanins and phenolics in the leaves and peduncles of MYB75/PAP1-D mutant were higher than in its WT line. 13 C excess in the extractives of leaves and peduncles of the mutant was also higher than that found in the WT line, indicating that the transcription factor MYB75 positively regulated carbon flow to flavonoids biosynthesis in the phenylpropanoid pathway. The myb75-1 mutant had higher distribution of 13 C in both extractives and nonextractives of leaves and peduncles, especially in nonextractives of the peduncles, and lower levels of anthocyanins compared with its WT. This suggests that the knockout of MYB75 inhibits biosynthesis of anthocyanins and regulates carbon flow from cytoplasm to cell wall components by activating the biosynthesis of monolignols in cytoplasm, which, in turn, are transported and deposited to the secondary cell wall, resulting in secondary cell wall thickening. Patterns of anthocyanin level and 13 C partitioning in roots were different from that seen in leaves and peduncles, suggesting that regulation of the phenylpropanoid pathway by MYB75 may be tissue specific. Résumé :On connaît mal le rôle des facteurs de transcription de la myéloblastose (FTM) dans la régulation du flux de carbone impliquant divers produits du sentier des phénylpropanoïdes. Les auteurs ont étudié, sur l'Arabidopsis thaliana (L.) Heynh., la répartition du carbone chez un mutant gain-de-fonction (MYB75/PAP1-D) et un mutant perte-de-fonction (myb75-1). La détermina-tion des teneurs en anthocyanes et en phénols totaux dans les tissus foliaires, pédonculaires, et racinaires chez ces mutants et chez le type sauvage (TS) a été évaluée, ainsi que la répartition du 13 C entre les substances extractibles (surtout du cytoplasme) et nonextractibles (surtout des parois cellulaires) de ces tissus. On observe de plus fortes teneurs en anthocyanes et en phénols dans les feuilles et les pédoncules chez le mutant MYB75/PAP1-D que chez la lignée TS. Les excès de 13 C dans les extractibles provenant des feuilles et des pédoncules du mutant sont également plus élevés que ceux de la lignée TS, ce qui indique que le facteur de transcription MYB75 régule positivement le flux de carbone vers la biosynthèse des flavonoïdes dans le sentier phénylpropanoïde. Quant au mutant myb75-1, il montre une plus forte distribution du 13 C dans les deux groupes, extractibles et nonextractibles des feuilles et des pédoncules, ...

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“…Cultivars, experimental lines, and generated double-haploid lines of B. napus indicated that C and N partitioning were affected by seed oil productivity (Fei et al 2013). Four genotypes of B. napus (cultivars Topas, Sentry and Polo, and the experimental line 04C204) varying in SOC potential from 42 to 50% seed dry weight were used to study C and N partitioning among shoots, roots and soil, as well as nitrogen use efficiency.…”

Section: General Findings and Relationship Withmentioning

confidence: 99%

The bilateral influence of plant and rhizosphere characteristics in brassicas varying in seed oil productivity

et al. 2014

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2014. The bilateral influence of plant and rhizosphere characteristics in brassicas varying in seed oil productivity. Can. J. Plant Sci. 94: 1113Á1116. It is important that increasing seed oil yield in species of Brassica to improve the crops as biodiesel feedstocks does not result in unforeseen increases in greenhouse gas (GHG) emissions. Studies were conducted to determine if genotypes of Brassica napus and Arabidopsis thaliana varying in seed oil content (SOC) potential had differences in plant and rhizospheric characteristics that could impact GHG emissions. Varying SOC productivity in B. napus resulted in changes in C and N partitioning within the plant, and in some cases had effects on N 2 O emission in the field. Although changes were observed in the composition of the rhizosphere of A. thaliana with modified SOC, there was also evidence that rhizospheric bacteria-to-plant signals could be used to improve growth and stress resistance in the plants. Project 4c in the Green Crop Network (GCN) investigated the possible ramifications of varying SOC on various plant growth, rhizospheric and agronomic characteristic of Brassica napus L. and Arabidopsis thaliana (L.) Heynh. The influence of certain bacteria-to-plant signals (i.e., lipo-chitooligosaccharides) was also investigated in these species. The rationale for these investigations was based on the fact that very little is known about how changing seed oil productivity in brassicas might affect other plants processes (e.g., C and N partitioning, root exudations, rhizospheric conditions) that might affect GHG emission from biodiesel feedstock crops designed specifically for maximized SOC.

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13C and 15N partitioning among shoots, roots, and soil in Brassica napus genotypes varying in seed oil content potential

Cited by 5 publications

References 39 publications

Nitrogen use efficiency in rapeseed. A review

Nitrogen use efficiency in rapeseed. A review

Carbon partitioning in tissues of a gain-of-function mutant (MYB75/PAP1-D) and a loss-of-function mutant (myb75-1) inArabidopsis thaliana

The bilateral influence of plant and rhizosphere characteristics in brassicas varying in seed oil productivity

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