130 Rapid detection of polymyxin resistance in Enterobacteriaceae

Rapid Polymyxin NP test for the detection of polymyxin resistance mediated by the mcr-1/mcr-2 genes

“…It is based on the detection of glucose metabolization associated with bacterial growth in the presence of a given concentration of polymyxin B or colistin. When growth occurs (resistant strain), formation of acid metabolism in less than 2 hours is evidenced by a color change of a pH indicator, red phenol (Nordmann et al, 2016). (See Fig.…”

Screening of plasmid-mediated MCR-1 colistin-resistance from bacteremia

“…The predominance of E. coli isolates in that collection is in accordance with the known enterobacterial distribution in bacteremia. The strains were screened first by using the recently-developed Rapid Polymyxin NP test that is more reliable and rapid to detect colistin resistance than disk diffusion, E-test and automated systems [4,5]. Then, all strains were screened for the mcr-1 gene by using regular and real-time PCR techniques [3,6].…”

MCR: modern colistin resistance

“…To date, the exact prevalence of the mcr-1 gene remains unknown. Benefits of culture-based polymyxin resistance screening and rapid detection of polymyxin resistance in Enterobacteriaceae have been pointed out [39,40]. To render the search for colistin resistance more clinically useful, simultaneous detection of carbapenem resistance should be considered.…”

“…The recently developed Super Polymyxin selective medium provides a useful tool for the screening of polymyxinresistant Gram-negative isolates [39]. Using a large collection of polymyxin-resistant isolates, it has been shown to possess close to 100 % sensitivity and specificity.…”

“…In order to increase the throughput, precision, and speed of colistin resistance testing, it would be good if (additional) assays for the commercially available automated AST systems could be developed or improved. The recently developed Rapid Polymyxin NP test is a tool that is available for accurate distinction between susceptible or resistant strains [39]. It may be implemented worldwide and is affordable.…”