Screening of plasmid-mediated MCR-1 colistin-resistance from bacteremia

Nordmann, Patrice; Assouvie, Laetititia; Prod’hom, Guy; Poirel, Laurent; Greub, Gilbert

doi:10.1007/s10096-016-2739-0

Cited by 14 publications

(6 citation statements)

References 8 publications

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“…Of these isolates, three (2.1%) carried mcr-1 genes. Our data showed that the low prevalence of the mcr-1 gene among E. coli isolates from human bloodstream infections was slightly higher than that observed (0–1%) from patients in other countries and regions,1,11,15,16 whereas Lai et al detected mcr-1 carriage in 75% of E. coli strains from pigs 11. Hence, the reservoir for mcr-1 -positive E. coli seems to be larger among animals than among humans.…”

Section: Discussioncontrasting

confidence: 60%

Epidemiology and molecular characterization of mcr-1 in Escherichia coli recovered from patients with bloodstream infections in Changsha, central China

Zhong¹,

Liu²,

Zheng³

2019

IDR

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Objectives The main aim of this study was to investigate the prevalence and molecular characteristics of the mcr-1 gene in Escherichia coli isolates obtained from all patients with bloodstream infections over a year in a Chinese teaching hospital. We also assessed the susceptibility profiles of the mcr-1 -positive strains and prognostic impact of this gene on the patients. Methods A total of 144 consecutive, non-repetitive E. coli isolates causing bloodstream infections were collected at a teaching hospital in Changsha, China from January to December 2016. The presence of the mcr-1 gene was assessed by PCR. All mcr-1 -positive E coli isolates were characterized by antimicrobial susceptibility testing, multilocus sequence typing (MLST), a conjugation experiment, and plasmid replicon typing. Clinical data were obtained from medical records. Results The mcr-1 gene was detected in three (2.1%) of the 144 E. coli isolates. The three mcr-1 -positive E. coli isolates were resistant to colistin. All three isolates showed a lower resistance to other classes of antibacterials, with all three being susceptible to carbapenems. The MLST results indicated that the three E. coli isolates were assigned to three different sequence types: ST457, ST101, and ST1413, respectively. The conjugation experiment showed that the mcr-1 gene was successfully transferred to the recipient ( E. coli EC600) from two isolates, one of which possessed IncI1 replicons and the other of which carried IncHI2 and IncN replicons. The patients with bloodstream infections caused by mcr-1 -positive isolates had severe underlying diseases and were cured after antibacterial treatment. Conclusion The prevalence of the mcr-1 gene in patients with E. coli bloodstream infection was 2.1% in Changsha, China. The mcr-1 -positive E. coli isolates had varied susceptibility profiles, although all three were susceptible to carbapenems. This therapeutic window is crucial given the risk of rapid deterioration in high-incidence areas worldwide.

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Section: Discussioncontrasting

confidence: 60%

Epidemiology and molecular characterization of mcr-1 in Escherichia coli recovered from patients with bloodstream infections in Changsha, central China

Zhong¹,

Liu²,

Zheng³

2019

IDR

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“…Prevalence of MCR-1–producing Enterobacteriacae isolates, mainly E. coli , from 2 pig farms in Portugal was high. So far, the mcr-1 gene has been identified mainly in animal samples, rarely in environmental and human samples ( 3 , 4 , 33 – 36 ). Previous studies describe the occurrence of MCR-1 producers in swine; prevalence in Europe ranged from 0.5% to 13.5% ( 6 , 8 , 36 , 37 ) and in China up to 20.6% ( 1 ).…”

Section: Discussionmentioning

confidence: 99%

High Rate of MCR-1–Producing Escherichia coli and Klebsiella pneumoniae among Pigs, Portugal

Kieffer¹,

Aires-de-Sousa²,

Nordmann³

et al. 2017

Emerg. Infect. Dis.

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The mcr-1 (mobile colistin resistance 1) gene, which encodes phosphoethanolamine transferase, has been recently identified as a source of acquired resistance to polymyxins in Escherichia coli. Using the SuperPolymyxin selective medium, we prospectively screened 100 pigs at 2 farms in Portugal for polymyxin-resistant Enterobacteriaceae and recovered 98 plasmid-mediated MCR-1–producing isolates. Most isolates corresponded to nonclonally related E. coli belonging to many sequence types; we also found 2 Klebsiella pneumoniae sequence types. The mcr-1 gene was carried on IncHI2 or IncP plasmid backbones. Our finding of a high rate of MCR-1 producers on 2 pig farms in Portugal highlights the diffusion of that colistin-resistance determinant at the farm level. The fact that the pigs received colistin as metaphylaxis in their feed during the 6 weeks before sampling suggests selective pressure.

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“…Recently, researchers have rushed to assess historical isolates in an effort to identify potential emergence dates for mcr and current reports have identified isolates harboring mcr-1 as far back as 1980 [ 6 ]. Worldwide reports have identified of mcr-1 in a range of Enterobacteriaceae from human and animal hosts including Escherichia coli , Salmonella , Klebsiella and other Gram negative organisms [ 7 – 14 ]. Researchers have identified the genomic locations of mcr-1 which include chromosomal integration [ 15 ], while others report that mcr-1 is mobile, being frequently linked with a range of plasmid types including Inc I2, Inc P, Inc FIP, Inc F and Inc HI2 as well as some Inc X4 types [ 1 , 3 , 5 , 14 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

mcr-1 identified in Avian Pathogenic Escherichia coli (APEC)

et al. 2017

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Antimicrobial resistance associated with colistin has emerged as a significant concern worldwide threatening the use of one of the most important antimicrobials for treating human disease.Here, we examined a collection (n = 980) of Avian Pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis from the US and internationally for the presence of mcr-1 and mcr-2, genes known to encode colistin resistance. Included in the analysis was an additional set of avian fecal E. coli (AFEC) (n = 220) isolates from healthy birds for comparative analysis. The mcr-1 gene was detected in a total of 12 isolates recovered from diseased production birds from China and Egypt. No mcr genes were detected in the healthy fecal isolates. The full mcr-1 gene from positive isolates was sequenced using specifically designed primers and were compared with sequences currently described in NCBI. mcr-1 positive isolates were also assessed for phenotypic colistin resistance and extended spectrum beta lactam phenotypes and genotypes. This study has identified mcr-1 in APEC isolates dating back to at least 2010 and suggests that animal husbandry practices could result in a potential source of resistance to the human food chain in countries where application of colistin in animal health is practiced.

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Screening of plasmid-mediated MCR-1 colistin-resistance from bacteremia

Cited by 14 publications

References 8 publications

<p>Epidemiology and molecular characterization of <em>mcr-1</em> in <em>Escherichia coli</em> recovered from patients with bloodstream infections in Changsha, central China</p>

<p>Epidemiology and molecular characterization of <em>mcr-1</em> in <em>Escherichia coli</em> recovered from patients with bloodstream infections in Changsha, central China</p>

High Rate of MCR-1–Producing Escherichia coli and Klebsiella pneumoniae among Pigs, Portugal

mcr-1 identified in Avian Pathogenic Escherichia coli (APEC)

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