[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA

Mandel, M.; Marmur, Julius

doi:10.1016/0076-6879(67)12133-2

Cited by 830 publications

(493 citation statements)

References 26 publications

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“…(i) Determination of GC content Two comparative methods were used: thermal denaturation and analytical isopycnic CsCl centrifugation (Mandel & Marmur, 1968). The hyperchromic shift of Stentor macronuclear DNA due to thermal denaturation in 0-1 SSC was about 35 % and T m (temperature for half denaturation) between 67 and 68 °C (Text- fig.…”

Section: Dna Characterization: Resultsmentioning

confidence: 99%

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Macronuclear DNA in Stentor coeruleus: a first approach to its characterization

Pelvat

Haller

1976

Genet. Res.

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SUMMARYThe macronuclei of Stentor coeruleus were isolated on a discontinuous sucrose gradient and their DNA was purified by conventional methods. The GC content was 32 mole %. The DNA banded as a single peak on analytical ultracentrifugation at 1-691 g/cm 3 . The molecular weight of the DNA was 5 x 10 6 to 4 x 10 7 daltons. Genome size determined by DNA-DNA reassociation kinetics was 6 x 10 10 daltons. The macronuclear genome was mostly simple, about 85 % being made of non-repetitive sequences.

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Section: Dna Characterization: Resultsmentioning

confidence: 99%

“…(v) Determination of thermal transition {thermal melting) of the DNA The general procedure was first described by Mandel & Marmur (1968) and Marmur & Doty (1962). Thermal melting being dependent on the ionic strength of.…”

Section: Materials and Methods (I) Culture Of Stentorsmentioning

confidence: 99%

Macronuclear DNA in Stentor coeruleus: a first approach to its characterization

Pelvat

Haller

1976

Genet. Res.

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“…22 The G þ C content of the DNA was determined using the thermal denaturation method. 23 DNA from Escherichia coli ATCC 11775 T and Vibrio azureus LMG 25266 T were used as a reference for determination of the thermal-melting profile (T m ).…”

Section: Genotypic Characterizationmentioning

confidence: 99%

Vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against Vibrio species

2012

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“…The DNA G+C composition of strain 25-1 T was determined using the thermal denaturation method (Mandel and Marmur, 1968) with Escherichia coli K-12 as a control. Genomic DNA was extracted and purified using conventional procedures (Sambrook and Russell, 2001).…”

Section: Chemotaxonomic and Genomic Analysesmentioning

confidence: 99%

分离自成都大熊猫兽舍空气中的一株金黄杆菌属新种的鉴定

Wen

Zhao

et al. 2016

J. Zhejiang Univ. Sci. B

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A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1 T , was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1 T grew optimally at pH 7.0-8.0, at 28-30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1 T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81 T (96.4% similarity), C. lathyri RBA2-6 T (95.8% similarity), and C. zeae JM1085 T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C 15:0 (44.0%), iso-C 17:0 3OH (19.8%) and C 16:1 ω7c/ 16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA-DNA relatedness between strain 25-1 T and C. lathyri RBA2-6 T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1 T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1 T (CCTCC AB2015133 T =DSM 100396 T ).

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[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA

Cited by 830 publications

References 26 publications

Macronuclear DNA in Stentor coeruleus: a first approach to its characterization

Macronuclear DNA in Stentor coeruleus: a first approach to its characterization

Vibrio inhibens sp. nov., a novel bacterium with inhibitory activity against Vibrio species

分离自成都大熊猫兽舍空气中的一株金黄杆菌属新种的鉴定

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