Assessment of the mechanics of a tissue-engineered rat trachea in an image-processing environment

Silva, Thiago Henrique Gomes da; Pazetti, Rogério; Aoki, Fabio Gava; Cardoso, Paulo Francisco Guerreiro; Valenga, Marcelo Henrique; Deffune, Elenice; Evaristo, Thaiane Cristine; Pêgo-Fernandes, Paulo Manuel; Moriya, Henrique Takachi

doi:10.6061/clinics/2014(07)11

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…Protocols of tracheal mechanics often differ and are performed in the most varied species (Table ), such as rabbits , sheep , dogs , rats , and pigs which makes it difficult to compare the results. Moreover, tracheal samples can be found in isolated parts or long segments of the organ.…”

Section: Different Mammals Used In the Study Of Tracheal Mechanicsmentioning

confidence: 99%

“…Some apparatuses can complement the experimental setup of the pressure–volume relationship and also computational models can be used for the research on tracheal mechanics. Imaging devices or lasers/LEDs can be used to obtain the dimensions of the trachea during the experiment and have the advantage of not requiring mechanical intervention for the measurement. Micrometers and video images have the disadvantage of measuring only 1D or 2D, limiting to at most only one cross‐section plane at a time.…”

Section: Macro Scalementioning

confidence: 99%

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Mechanical Evaluation of Tracheal Grafts on Different Scales

Aoki

Moriya

2017

Artificial Organs

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Tissue engineered (or bioengineered) tracheas are alternative options under investigation when the resection with end-to-end anastomosis cannot be performed. One approach to develop bioengineered tracheas is a complex process that involves the use of decellularized tissue scaffolds, followed by recellularization in custom-made tracheal bioreactors. Tracheas withstand pressure variations and their biomechanics are of great importance so that they do not collapse during respiration, although there has been no preferred method of mechanical assay of tracheas among several laboratories over the years. These methods have been performed in segments or whole tracheas and in different species of mammals. This article aims to present some methods used by different research laboratories to evaluate the mechanics of tracheal grafts and presents the importance of the tracheal biomechanics in both macro and micro scales. If bioengineered tracheas become a reality in hospitals in the next few years, the standardization of biomechanical parameters will be necessary for greater consistency of results before transplantations.

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Section: Different Mammals Used In the Study Of Tracheal Mechanicsmentioning

confidence: 99%

Section: Macro Scalementioning

confidence: 99%

Mechanical Evaluation of Tracheal Grafts on Different Scales

Aoki

Moriya

2017

Artificial Organs

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“…Image processing of tracheae to assist in mechanical evaluation seems to be useful as it permits both in situ and in vivo assessments, and also allows dynamic evaluation of organs and tissues without the need for physical contact . Different modalities of imaging have been used in order to find the best way to overcome limitations regarding temporal and spatial resolution …”

Section: Introductionmentioning

confidence: 99%

An in vivo image acquisition system for the evaluation of tracheal mechanics in rats

et al. 2019

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Mechanical evaluation of tracheal grafts is of great relevance for transplant research.Although there are some publications demonstrating different techniques of tracheal mechanical evaluation, there is still no definitive or preferred protocol available. Here, we present a simple image processing acquisition system that can be used for in vivo experiments. Six male Wistar rats were submitted to orotracheal intubation and a longitudinal incision was made to expose the trachea. Images of tracheae were acquired from a video camera in different scenarios of bronchoconstriction using methacholine (MCh) (Basal, PBS, MCh 30 μg/kg, MCh 300 μg/kg, and postmetabolized) during imposed-inspiration and imposed-expiration. The area variation ratio (the ratio between areas during expiration vs. inspiration) was 1.1× for the Basal group, while the ratio for MCh 300 µg/kg was 6.5×. The area variation of imaged tracheae was statistically significant at the dose of MCh 300 µg/kg for imposed-inspiration versus imposed-expiration (P = .002). Likewise, elastance data of respiratory mechanics indicated a statistically significant difference at the dose of MCh 300 µg/kg for imposed-inspiration versus imposed-expiration (P = .026). Our image processing analysis protocol presented corresponding behavior when compared to mechanical parameters of the respiratory system. In addition, our image acquisition system was able to highlight the differences between imposed-inspiration and imposed-expiration. Image analysis of the tracheal area variation seems to be in agreement with the elastance of the respiratory system. Taken together, these observations may help future studies of tracheal transplantation for in situ assessment of graft patency.

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“…However, the cost and quality of using EVLP donor tissue in our country, and as a result, other transplants have not been performed with this technique. New research has been implemented in this area that incorporated the use of broad spectrum antibiotics during EVLP 19 and in trachea transplantation 20 , 21 .…”

mentioning

confidence: 99%