Mechanical Evaluation of Tracheal Grafts on Different Scales

Aoki, Fabio Gava; Moriya, Henrique Takachi

doi:10.1111/aor.13063

Cited by 7 publications

(9 citation statements)

References 99 publications

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“…Although some studies have applied objective techniques, such as microscopic evaluation of the tissue, this is insufficient as it assesses only one part of the trachea (e.g., muscle, cartilage, mucosa, etc.) rather than the whole piece, which is the main point of interest in a tracheal substitute [25,26].…”

Section: Discussionmentioning

confidence: 99%

A Standardised Approach to the Biomechanical Evaluation of Tracheal Grafts

et al. 2021

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The ideal tracheal substitute must have biomechanical properties comparable to the native trachea, but currently there is no standardised approach to evaluating these properties. Here we propose a novel method for evaluating and comparing the properties of tracheal substitutes, thus systematising both measurement and data curation. This system was tested by comparing native rabbit tracheas to frozen and decellularised specimens and determining the histological characteristics of those specimens. We performed radial compression tests on the anteroposterior tracheal axis and longitudinal axial tensile tests with the specimens anastomosed to the jaw connected to a measuring system. All calculations and results were adjusted according to tracheal size, always using variables relative to the tracheal dimensions, thus permitting comparison of different sized organs. The biomechanical properties of the decellularised specimens were only slightly reduced compared to controls and significant in regard to the maximum stress withstood in the longitudinal axis (−0.246 MPa CI [−0.248, −0.145] MPa) and the energy stored per volume unit (−0.124 mJ·mm−3 CI [−0.195, −0.055] mJ·mm−3). The proposed method is suitable for the systematic characterisation of the biomechanical properties of different tracheal substitutes, regardless of the size or nature of the substitute, thus allowing for direct comparisons.

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Section: Discussionmentioning

confidence: 99%

A Standardised Approach to the Biomechanical Evaluation of Tracheal Grafts

et al. 2021

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“…Evaluation of tracheal biomechanics is important in the analysis of decellularized tracheal grafts 38 and often used as a measure to predict tracheal collapse. Grafts that mimic the native trachea are deemed ideal.…”

Section: Discussionmentioning

confidence: 99%

De-epithelialization of porcine tracheal allografts as an approach for tracheal tissue engineering

Aoki

Varma

Marin-Araujo

et al. 2019

Sci Rep

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Replacement of large tracheal defects remains an unmet clinical need. While recellularization of acellular tracheal grafts appeared to be a viable pathway, evidence from the clinic suggests otherwise. In hindsight, complete removal of chondrocytes and repopulation of the tracheal chondroid matrix to achieve functional tracheal cartilage may have been unrealistic. In contrast, the concept of a hybrid graft whereby the epithelium is removed and the immune-privileged cartilage is preserved is a radically different path with initial reports indicating potential clinical success. Here, we present a novel approach using a double-chamber bioreactor to de-epithelialize tracheal grafts and subsequently repopulate the grafts with exogenous cells. A 3 h treatment with sodium dodecyl sulfate perfused through the inner chamber efficiently removes the majority of the tracheal epithelium while the outer chamber, perfused with growth media, keeps most (68.6 ± 7.3%) of the chondrocyte population viable. De-epithelialized grafts support human bronchial epithelial cell (BEAS-2B) attachment, viability and growth over 7 days. While not without limitations, our approach suggests value in the ultimate use of a chimeric allograft with intact donor cartilage re-epithelialized with recipient-derived epithelium. By adopting a brief and partial decellularization approach, specifically removing the epithelium, we avoid the need for cartilage regeneration.

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“…Fabio Gava Aoki and Henrique Takachi Moriya of the University of São Paulo, São Paulo, Brazil, reviewed the mechanical properties evaluations of tracheal grafts. They present the methods used by different research laboratories to evaluate the mechanics of tracheal grafts and present the importance of the tracheal biomechanics in both macro and micro scales.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Artificial Organs 2018: A Year in Review

Malchesky¹

2019

Artificial Organs

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In this Editor's Review, articles published in 2018 are organized by category and summarized. We provide a brief reflection of the research and progress in artificial organs intended to advance and better human life while providing insight for continued application of these technologies and methods. Artificial Organs continues in the original mission of its founders “to foster communications in the field of artificial organs on an international level.” Artificial Organs continues to publish developments and clinical applications of artificial organ technologies in this broad and expanding field of organ Replacement, Recovery, and Regeneration from all over the world. Peer‐reviewed special issues this year included contributions from the 13th International Conference on Pediatric Mechanical Circulatory Support Systems and Pediatric Cardiopulmonary Perfusion edited by Dr. Akif Undar, and the 25th Congress of the International Society for Mechanical Circulatory Support edited by Dr. Marvin Slepian. Additionally, many editorials highlighted the worldwide survival differences in hemodialysis and perspectives on mechanical circulatory support and stem cell therapies for cardiac support. We take this time also to express our gratitude to our authors for offering their work to this journal. We offer our very special thanks to our reviewers who give so generously of time and expertise to review, critique, and especially provide meaningful suggestions to the author's work whether eventually accepted or rejected. Without these excellent and dedicated reviewers the quality expected from such a journal could not be possible. We also express our special thanks to our Publisher, John Wiley & Sons for their expert attention and support in the production and marketing of Artificial Organs. We look forward to reporting further advances in the coming years.

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Mechanical Evaluation of Tracheal Grafts on Different Scales

Cited by 7 publications

References 99 publications

A Standardised Approach to the Biomechanical Evaluation of Tracheal Grafts

A Standardised Approach to the Biomechanical Evaluation of Tracheal Grafts

De-epithelialization of porcine tracheal allografts as an approach for tracheal tissue engineering

Artificial Organs 2018: A Year in Review

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