Efficacy of Papacarie® in reduction of residual bacteria in deciduous teeth: a randomized, controlled clinical trial

Abstract

OBJECTIVES:The aim of the present study was to analyze the efficacy of Papacarie® gel compared with the traditional method (low-speed bur) in reducing the counts of total bacteria, Lactobacillus, total Streptococcus and Streptococcus mutans group.METHODS:A randomized, controlled clinical trial with a split-mouth design was performed. The sample comprised 40 deciduous teeth in 20 children (10 males and 10 females) aged four to seven years. The teeth were randomly allocated to two groups: G1, or chemomechanical caries removal with Papacarie Duo®, and G2, or the removal of carious dentin tissue with a low-speed bur. Infected dentin was collected prior to the procedure, and the remaining dentin was collected immediately following the removal of the carious tissue. Initial and final counts of bacterial colonies were performed to determine whether there was a reduction in the number of colony-forming units (CFUs) of each microorganism studied. ClinicalTrials.gov: NCT01811420.RESULTS:Reductions were found in the numbers of total bacteria, total Streptococcus and Streptococcus mutans group following either of the caries removal methods (p<0.05). A reduction was also noted in the number of Lactobacillus CFUs; however, this difference did not achieve statistical significance (p>0.05).CONCLUSION:Papacarie® is an excellent option for the minimally invasive removal of carious tissue, achieving significant reductions in total bacteria, total Streptococcus and S. mutans with the same effectiveness as the traditional caries removal method.

Efficacy and Patient’s Acceptance of Alternative Methods for Caries Removal—A Systematic Review

“…Two [ 26 , 27 ] included studies followed a cross-over trial design, while eighteen [ 10 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ] had a split-mouth design. One study [ 45 ] followed a two parallel-arms trial, five studies [ 46 , 47 , 48 , 49 , 50 ] provided one treatment per patient while five studies [ 15 , 51 , 52 , 53 , 54 ] had one or more treatment per patient.…”

Effects of Papacarie on children with dental caries in primary teeth: a systematic review and meta‐analysis

“…The trials’ publication date was from 2009 to 2016. Among these trials, three articles reported the same experiment; therefore, they were combined as Motta 2013–2014. Different regimens of the Papacarie method were identified in the included articles.…”

Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells

“…Some materials are biocompatible with the dentin-pulp complex when applied to superficial and moderate cavities, but when applied to deeper cavities may be toxic to the pulp tissue or may adversely affect the repair process (6). Papacárie Duo® is a material developed for chemo-mechanical caries removal and consists mainly of papain (7), which has antimicrobial properties (3,8,9). This papain-based gel is basically composed of a proteolytic enzyme that interacts with the partially degraded collagen present in the necrotic carious tissue (7).…”