Effects of ovariectomy and resistance training on oxidative stress markers in the rat liver

Abstract

OBJECTIVE:The objective of this study was to assess the effects of resistance training on oxidative stress markers in the livers of ovariectomized rats.METHOD:Adult Sprague-Dawley rats were divided into the following four groups (n = 8 per group): sham-operated sedentary, ovariectomized sedentary, sham-operated resistance training, and ovariectomized resistance training. During the resistance training period, the animals climbed a 1.1-m vertical ladder with weights attached to their tails; the sessions were conducted 3 times per week, with 4-9 climbs and 8-12 dynamic movements per climb. The oxidative stress was assessed by measuring the levels of reduced glutathione and oxidized glutathione, the enzymatic activity of catalase and superoxide dismutase, lipid peroxidation, vitamin E concentrations, and the gene expression of glutathione peroxidase.RESULTS:The results showed significant reductions in the reduced glutathione/oxidized glutathione ratio (4.11±0.65 nmol/g tec), vitamin E concentration (55.36±11.11 nmol/g), and gene expression of glutathione peroxidase (0.49±0.16 arbitrary units) in the livers of ovariectomized rats compared with the livers of unovariectomized animals (5.71±0.71 nmol/g tec, 100.14±10.99 nmol/g, and 1.09±0.54 arbitrary units, respectively). Moreover, resistance training for 10 weeks was not able to reduce the oxidative stress in the livers of ovariectomized rats and induced negative changes in the hepatic anti-oxidative/oxidative balance.CONCLUSION:Our findings indicate that the resistance training program used in this study was not able to attenuate the hepatic oxidative damage caused by ovariectomy and increased the hepatic oxidative stress.

Estrogen deficiency in ovariectomized rats: can resistance training re-establish angiogenesis in visceral adipose tissue?

“…Ovx animals have been used as models for studying the effects of ovarian hormone deficiency 18,25. Evidence from rodent models has demonstrated that ovariectomy promotes obesity and its metabolic complications, in particular insulin resistance 26,27.…”

Effects of Ovariohysterectomy on Oxidative Stress Markers in Female Dogs

“…; Rodrigues et al. ). Oestrogens are known to have antioxidant properties and their deficiency after ovariectomy predisposes the body to increased ROS production (Akcay et al.…”

“…; Rodrigues et al. ). A higher risk of many diseases associated with oxidative stress, such as cardiovascular diseases, renal diseases, osteoporosis and Parkinsonism, has been observed in women after ovariectomy (Silbiger and Neugarten ; Astma et al.…”

Unraveling the Role of Melatonin/Quercetin in Attenuating the Metabolic and Bone Turnover Alternations in Iron Treated-Ovariectomized Female Rats

“…Accordingly, our findings revealed a significantly increased MDA level associated with a significantly decreased TAC in the sera and livers of OVX group compared to the sham group, and of F-OVX group compared to OVX group; revealed an ovariectomy-induced oxidative stress, further exaggerated by excess iron accumulation. In harmony, enhancement of oxidative stress response in ovariectomized [62,63], and iron overloaded conditions [29,64] were previously recorded, which could be a key process involved in mediating the pathology induced by ovariectomy and/or excess iron accumulation.…”