Effects of Ovariohysterectomy on Oxidative Stress Markers in Female Dogs

Abstract

Numerous studies reported an increase of oxidative stress increases in both women and female laboratory animals after ovariectomy. However, there is little information about the evaluation of antioxidative/oxidative status in ovariectomized dogs. The purpose of this study was to examine the changes in oxidative stress markers after ovariohysterectomy (OHE) in female dogs. The study included eighteen healthy mongrel female dogs. Blood samples were collected immediately before surgery and 14 and 30 days after surgery. Following parameters of oxidative stress intensity were determined: the erythrocyte activity of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) as well as the plasma concentrations of thiobarbituric acid reactive substances (TBARS), radical cations of N,N, diethylpara-phenylene diamine (RC-DEPPD), sulfhydryl groups (SH groups), bityrosine and formylkynurenine. The activity of GSH-Px increased markedly, although not significantly, 14 days after OHE and then significantly decreased at 30 days after OHE. A significant increase in plasma TBARS, bityrosine and formylkynurenine concentrations and a decrease in SH group content were concurrently noted at 30 days after surgery. Acquired results suggested that a loss of control over ROS production occurred in female dogs after OHE, which could lead to oxidative stress in the late post-operative period. In conclusion, our findings indicated that OHE is related with the risk of oxidative stress in the late period after operations. Given that oxidative stress contributes to the pathogenesis of various diseases, this may suggest an increased risk of disorders in ovariectomized female dogs; however, further studies are necessary to confirm this hypothesis.

Changes in the oxidative stress factors and inflammatory proteins following the treatment of BPH‐induced dogs with an anti‐proliferative agent called tadalafil

“…Higher effects of these enzymes were detected at 14 days after the operation (Szczubial, Kankofer, Bochniarz, & Dąbrowski, 2015).…”

“…Nevertheless, some researchers have found that oxidative enzymes do not differ significantly during the preoperative period and the following postoperative period, and the reduction in their activities has been attributed to the neutralization of excessive amounts of ROS generated after an operation. Higher effects of these enzymes were detected at 14 days after the operation (Szczubial, Kankofer, Bochniarz, & Dąbrowski, ). Different studies have shown an increase in the MDA concentration in animals with operation compared to the concentration observed in the control groups (Topçuoglu et al, ).…”

Gonadectomy effects on the risk of immune disorders in the dog: a retrospective study

“…A similar anti-inflammatory effect may occur in the dog. Additionally, estradiol has an antioxidant effect in mice [70] and a recent study in dogs reported that gonadectomized females have a greater risk for oxidative stress [72] which may lead to greater susceptibility for immune diseases.…”

Köpeklerde Pyometranın Bazı Oksidatif Stres Parametreleri, İnflamatorik Mediyatörler ve Nötrofil Segmentasyonu Üzerine Etkileri

“…According to our results none of the groups did show oxidative stress before and after OVH and additionally CAT and TBARS values were elevated after 15 days of OVH exception of SOD value. In another study [35] in healthy bitches, OVH was related with oxidative stress after 30 days of the surgeries. Our results have shown accordance with the earlier results of that study.…”