Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation

Hamid, Adila A.; Idrus, Ruszymah Bt Hj; Saim, Aminuddin Bin; Sathappan, S; Chua, Kien Hui

doi:10.6061/clinics/2012(02)03

Cited by 73 publications

(64 citation statements)

References 26 publications

(30 reference statements)

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“…However, a significantly elevated expression of collagen type X was observed after 3 weeks of chondrogenic induction [41,81]. The tendency of the AMSCs to differentiate in hypertrophic chondrocytes had been further confirmed by the other studies.…”

Section: In Vitro Studiessupporting

confidence: 65%

See 1 more Smart Citation

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Toumi¹,

Lespessailles²,

Mazor³

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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Although many surgical and pharmaceutical interventions are currently available for treating osteoarthritis (OA), restoration of normal cartilage function remains inefficient.In fact, because of the absence of vasculature within the articular cartilage (AC), the selfpotential for regeneration is very poor. Recently, researchers and clinicians have been focusing on alternative methods for cartilage preservation and repair. It has been shown that AC contains a population of stem cells or progenitor cells, similar to those found in many other adult tissues that are thought to be involved in the maintenance of tissue homeostasis. In the present chapter, we review the current status of stem cells potential in the treatment of early OA and discuss the possible origin of these cells and the role they might have in cartilage repair. We also review the recent progress in the field of chondroprogenitors in cartilage.

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Section: In Vitro Studiessupporting

confidence: 65%

“…Chondrogenesis of human AMSCs has shown significantly higher expression of chondrogenic markers after 1 week under appropriate conditions [81]. However, a significantly elevated expression of collagen type X was observed after 3 weeks of chondrogenic induction [41,81].…”

Section: In Vitro Studiesmentioning

confidence: 99%

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Toumi¹,

Lespessailles²,

Mazor³

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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“…Besides, HMG supplementation also caused a gradual increase in SOX-9 and aggrecan core protein (ACP) expression in cultured HACs compared to the control group. SOX-9 is a transcription factor in chondrogenesis, and its expression is essential for the anabolic activity of the chondrocyte as well as the differentiation of stem cells [32]. While ACP is a major component of the cartilage matrix which helps the cartilage in sustaining continuous mechanical loads [33].…”

Section: Discussionmentioning

confidence: 99%

Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study

Chua

Lee

Nagandran

et al. 2013

BMC Complement Altern Med

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BackgroundOsteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird’s Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.MethodsA single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes’ expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.ResultsMTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes’ expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.ConclusionEdible Bird’s Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.

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“…Research groups who analyzed the gene expression of biomarkers during embryogenesis and chondrogenic induction of MSCs found that SOX9 expression starts at the mesenchymal osteochondroprogenitor stage and remains elevated in all differentiated chondrocytes until the cells reach hypertrophy [49]. Hamid and colleagues subjected hASCs to chondrogenic differentiation and observed a peak of mRNA level of COL2A1, ACAN, and COMP after 1 week of induction, while the peak of SOX9 was found at three weeks [50]. Xu and colleagues studied the temporal changes in chondrogenic genes and developed a staging scheme for in vitro differentiation of MSCs in three-dimensional alginate gels.…”

Section: Discussionmentioning

confidence: 99%

Chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor

Pirrone¹,

Gobbetti²,

Caprara³

et al. 2017

J Stem Cell Res Med

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In recent years, various clinical trials are exploring the efficacy of cell-based strategies to treat cartilage defects. Human adipose-derived stem/stromal cells (hASCs) have great potential for the regeneration of articular cartilage due to their ability to undergo differentiation into the chondrogenic lineage. In most cell-based therapies, the single dose requires 10 7 -10 9 cells, and then it is necessary to move from manual flask-based culture to bioreactor whose monitoring and control of biological variability is necessary. Furthermore, before clinical use, the success of hASC differentiation need to be thoroughly inspected. In this study, we sought to assess with complementary methods the chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor (HFBR). Human ASCs, after phenotypic characterization were subjected to chondrogenic differentiation, the expression of cartilage-specific genes was analysed by quantitative real-time polymerase chain reaction (qPCR); optical microscopy as well as transmission electron microscopy (TEM) were also performed. The two culture expansion systems do not affect hASC immunophenotype; microscopy analysis confirmed the successful of hASC differentiation process. Gene expression analysis after differentiation displayed an upregulation of cartilage-specific genes more noticeable in hASCs expanded in the HFBR, suggesting that hASCs expanded in the HFBR can be efficiently differentiated into chondroblasts. Our study demonstrates that flask and HFBR expansion methods do not affect the subsequent differentiation process; additionally, our results highlight the importance of analyzing differentiation by different techniques and support the importance of TEM analysis that is able to give additional critical information to molecular biology results.

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Characterization of human adipose-derived stem cells and expression of chondrogenic genes during induction of cartilage differentiation

Cited by 73 publications

References 26 publications

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Stem Cell-Based Therapies for Osteoarthritis: From Pre-Clinical to Clinical Applications

Edible Bird’s nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: in vitro study

Chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor

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