Bradycardic and Antiarrhythmic Effects of the D-Limonene in Rats

Nascimento, Gildenisson Araújo do; Souza, Diego Santos; Lima, Bruno S.; Vasconcelos, Carla Maria Lins de; Araújo, Adriano Antunes de Souza; Durço, Aimée Obolari; Quintans‐Júnior, Lucindo José; Almeida, Jackson Roberto Guedes da Silva; Oliveira, Aldeída Pereira; Santana‐Filho, Valter Joviniano; Barreto, André S.; Santos, Márcio Roberto Viana dos

doi:10.5935/abc.20190173

Cited by 18 publications

(15 citation statements)

References 24 publications

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“…CCl 4 toxicity resulted in enhanced leakage of CK, CK-MB, and troponin-t, which are in line with the previous research that CCl 4 causes cardiac tissue disruption and secretion of CK-MB and troponin-t into the serum [ 58 , 59 ]. A significant reduction was observed in CK, CK-MB, and troponin-t level biomarkers of cardiac function with D-limonene (200 mg/kg body weight) suggesting improved cardiac function which is in concurrence with the findings that limonene protects against cardiovascular disease by lowering hypertension and cardiac arrhythmias [ 30 , 57 ]. However, these biochemical parameters which include CK, CK-MB, and troponin-t are just biomarkers of cardiac damage/injury.…”

Section: Discussionsupporting

confidence: 79%

“…Lipid and protein peroxidation follows myocardial cell membrane injury resulting in leakage of various enzymes like CK, CK-MB, LDH, Hs-CRP into the bloodstream and are increased after CCl 4 administration as reported previously [ 12 , 56 ]. Therefore, mitigating the disturbance of cardiomyocyte cell membrane antioxidants like D-limonene may prevent cardiomyocyte injury induced by CCl 4 and subsequent leakage of cellular enzymes [ 57 ]. CCl 4 toxicity resulted in enhanced leakage of CK, CK-MB, and troponin-t, which are in line with the previous research that CCl 4 causes cardiac tissue disruption and secretion of CK-MB and troponin-t into the serum [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

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D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl₄-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

et al. 2022

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Background: The cardiovascular crisis is advancing rapidly throughout the world. A large number of studies have shown that plant polyphenols affect major mechanisms involved in cardiovascular events through their action on the antioxidant system, signaling, and transcription pathways. D-limonene, a monocyclic monoterpene obtained from citrus fruits, is reported to possess many pharmacological activities. Methods: The experiment was designed to determine the protective effect of D-limonene against cardiac injury induced by CCl 4 in Wistar rats. Rats were treated with two doses of D-limonene against cardiac injury induced by CCl 4 . Serum toxicity markers, cardiac toxicity biomarker enzymes, inflammatory mediators, anti-oxidant armory, lipid peroxidation, lipid profile, and histology were done. Results: CCl 4 intoxication resulted in a substantial rise in FFA, TC, TG, PL, LDL, VLDL, and a reduction in HDL, restoring these changes with the administration of D-limonene at a dosage of 200 mg/kg. CCl 4 administration also resulted in lipid oxidation and decreased antioxidant activity. At the same time, D-limonene at a dosage of 200 mg/kg body weight inhibited LPO and restored in vivo antioxidant components to normal. CC l 4 intoxication also resulted in a significant increase in inflammatory markers like IL-6, TNF-α, high sensitivity Corticotropin Releasing Factor (Hs-CRF), and biomarkers of cardiac toxicity like alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatine kinase (CK), creatine kinase MB (CKMB), and Troponin I & troponin-t activities. D-limonene reversed all these changes to normal. Histology further confirmed our obtained results. Conclusion: These findings indicate that D-limonene can ameliorate cardiac injury at a 200 mg/kg body weight dosage. Henceforth, D-Limonene intervenes in mediating CCl 4 induced toxicity by various signaling pathways.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl₄-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

et al. 2022

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“…D-limonene in Wistar rats caused the intense and persistent bradycardia associated with hypotension. In the in vivo model of arrhythmia, D-limonene (10 mg/kg) reduced the heart rate and arrhythmia [ 40 ]. In experiments with Oreochromis niloticus , Citrus bergamia peel oil containing limonene and linalool was added to the fish diet.…”

Section: Terpenoids As Potential Geroprotectorsmentioning

confidence: 99%

Terpenoids as Potential Geroprotectors

et al. 2020

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Terpenes and terpenoids are the largest groups of plant secondary metabolites. However, unlike polyphenols, they are rarely associated with geroprotective properties. Here we evaluated the conformity of the biological effects of terpenoids with the criteria of geroprotectors, including primary criteria (lifespan-extending effects in model organisms, improvement of aging biomarkers, low toxicity, minimal adverse effects, improvement of the quality of life) and secondary criteria (evolutionarily conserved mechanisms of action, reproducibility of the effects on different models, prevention of age-associated diseases, increasing of stress-resistance). The number of substances that demonstrate the greatest compliance with both primary and secondary criteria of geroprotectors were found among different classes of terpenoids. Thus, terpenoids are an underestimated source of potential geroprotectors that can effectively influence the mechanisms of aging and age-related diseases.

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“…Os terpenos mostraram-se como os principais compostos com atividade antiarrítmica comprovada. [6][7][8][9] Entre eles, a carvona (p-mentha-6,8-dien-2-one) é de particular interesse, dadas suas propriedades já estabelecidas. A carvona é uma cetona pertencente ao grupo dos monoterpenos, e conhecida por sua atividade antioxidante, antimicrobiana, e antifúngica.…”

Section: Introductionunclassified

A (-)-Carvona Modula a Sinalização de Cálcio Intracelular com Ação Antiarrítmica em Corações de Ratos

Silva¹,

Souza²,

Menezes-Filho³

et al. 2022

Arquivos Brasileiros De Cardiologia

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Resumo Fundamento: A (-)-carvona é um monoterpeno encontrado em óleos essenciais com atividade antioxidante e anti-inflamátoria. Objetivos: O objetivo deste estudo foi analisar a propriedade antiarrítmica da (-)-carvona no coração de rato e seus efeitos sobre a sinalização de Ca +2 intracelular. Métodos: Os efeitos da (-)-carvona foram avaliados sobre a contratilidade atrial (0,01 – 4 mM) e ventricular (0,5 mM), e no eletrocardiograma (0,5mM). A fração de encurtamento, a corrente de cálcio do tipo L (I Ca,L ) e a sinalização de Ca +2 foram medidas no cardiomiócito isolado (0,5 mM). O efeito antiarrítmico foi avaliado no modelo de arritmia induzida por sobrecarga de cálcio (0,5 mM) (n = 5). Um p < 0,05 foi adotado como nível de significância estatística. Resultados: No átrio, a (-)-carvona causou inotropismo negativo de maneira concentração-dependente (EC 50 0,44 ± 0,11 mM) e diminuiu o inotropismo positivo induzido pelo CaCl 2 (0,1 – 8,0 mM) e BAY K8644 (5 - 500 nM), um agonista de canal de cálcio do tipo L. Em coração isolado, a (-)-carvona (0,5mM) reduziu a contratilidade ventricular em 73% e a frequência cardíaca (em 46%), aumentou o Pri (30,7%, tempo desde o início da onda P até a onda R) e o QTc (9,2%, uma medida de despolarização e repolarização dos ventrículos), sem mudar a duração do complexo QRS. A (-)-carvona diminuiu a fração de encurtamento (61%), a (I Ca,L ) (79%) e o transiente intracelular de Ca +2 (38%). Além disso, a (-)-carvona apresentou ação antiarrítmica, identificada pela redução do escore de arritmia (85%) e ocorrência de fibrilação ventricular. Conclusão: A (-)-carvona reduz a entrada de Ca +2 através de canais de Ca +2 do tipo L e, assim, diminui a contratilidade cardíaca e o Ca +2 intracelular e apresenta promissora atividade antiarrítmica no coração de ratos.

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Bradycardic and Antiarrhythmic Effects of the D-Limonene in Rats

Cited by 18 publications

References 24 publications

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl₄-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl₄-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

Terpenoids as Potential Geroprotectors

A (-)-Carvona Modula a Sinalização de Cálcio Intracelular com Ação Antiarrítmica em Corações de Ratos

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Bradycardic and Antiarrhythmic Effects of the D-Limonene in Rats

Cited by 18 publications

References 24 publications

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl4-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl4-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

Terpenoids as Potential Geroprotectors

A (-)-Carvona Modula a Sinalização de Cálcio Intracelular com Ação Antiarrítmica em Corações de Ratos

Contact Info

Product

Resources

About

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl₄-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers

D-limonene (5 (one-methyl-four-[1-methylethenyl]) cyclohexane) diminishes CCl₄-induced cardiac toxicity by alleviating oxidative stress, inflammatory and cardiac markers