Light and culture medium formulations for in vitro germination and development of Brassavola perrinii

Abstract

The improvement of in vitro propagation techniques using appropriate artifi cial light sources and culture medium formulations is important to allow the mass production of individuals with high genetic variability. Thus, this research aimed to study the effect of light sources from fl uorescent lamps and light-emitting diodes (LEDs) and culture medium formulations on the germination and initial development of the orchid species Brassavola perrinii. The experiment was set up in a completely randomized design in a 5 × 4 factorial scheme, consisting of fi ve light sourcesfl uorescent lamp (100%), white LED (100%), blue LED (100%), red LED (100%), and blue LED (50%) + red LED (50%) -and four culture medium formulations -MS, ½ MS, VW, and K -, with four replications and a mean of 125 seeds per plot. The percentage of germination, chlorophyll protocorms, and protocorm development through the classes P1, P2, P3, and P4 to calculate the protocorm development index were evaluated at 90 days after sowing. The data were subjected to analysis of variance and the means were compared by the Tukey test (p < 0.05). The percentage of germination for B. perrinii was not infl uenced by light spectra but was higher in the ½ MS and VW formulations. The highest protocorm development index of B. perrinii was observed when the species was submitted to the light spectrum of the lighting source with fl uorescent lamps in the VW culture medium.

Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose

“…Despite the widespread use of in vitro cultivation techniques for orchids, the in vitro propagation of Brassavola species still lacks established protocols, primarily due to the limited number of studies focused on these species (Pereira et al, 2022;Xu et al, 2022;Vendrame et al, 2023). Regarding B. tuberculata, only a few scientific articles have been published on its in vitro germination (Herrmann et al, 2011;Soares et al, 2012Soares et al, , 2020Rosa et al, 2013), seed storage and viability (Macedo et al, 2014;Soares et al, 2014;Sousa et al, 2020), multiplication and in vitro rooting (Mengarda et al, 2017), and ex vitro acclimatization (Sousa et al, 2015).…”

In vitro and ex vitro production of Schomburgkia crispa: effect of flask sealing systems and different light sources

“…Among the techniques, the in vitro germination of orchid seeds stands out since this type of sowing leads to high percentages of germination compared to germination under natural conditions, which occurs only in the presence of mycorrhizal fungi (Zhang et al 2018;Soares et al 2020;Kumar et al 2022). In this sense, the production of orchids by that method is important, as it allows a large number of seedlings to grow in a small space, in a short time, and with a high sanitary quality (Teixeira da Silva et al 2015Silva et al , 2017aFerreira et al 2022;Pereira et al 2022;Nowakowska et al 2022).…”