Establishment of in vitro aseptic culture of Philodendron xanadu Croat

Abstract

The philodendron is a plant with a high sales price; however, in Mexico there is not enough plant material available for the producers of this ornamental. Therefore, the objective of this research was to establish an in vitro aseptic culture of philodendron; for this, the effect of fungicides and bactericides applied from the transplantation of mother plants to the production and harvest of explants (axillary shoots) for in vitro establishment was evaluated. Two experiments were carried out using MS (MURASHIGE; SKOOG, 1962) culture medium; in the second, silver nanoparticles (AgNPs) were added to the culture medium. Both experiments were established in a completely randomized experimental design. It was observed that with the joint action of the sanitizing products in the treatment of mother plants and in the disinfection process, as well as the addition of AgNPs to the culture medium, it was possible to establish an aseptic culture of philodendron. Results show that 2 g L -1 of Agry-Gent Plus 5000 ® plus 2 g L -1 of Prozycar ® in the treatment of mother plants was the one generated the highest percentage of aseptic explants (25.71%) in the first in vitro establishment, while, in the second experiment, the same products plus the addition of AgNPs to the culture medium for seven days generated 100% aseptic explants. The contaminants identified were yeasts, Aspergillus sp. and Penicillium sp.

In Vitro Propagation of Philodendron erubescens ‘Pink Princess’ and Ex Vitro Acclimatization of the Plantlets

“…Several plant species of the genus Philodendron have been propagated via in vitro cultures, such as Philodendron oxycardium [12], Philodendron cannifolium [13], Philodendron selloum [14,15], Philodendron bipinnatifidum [16], Philodendron xanadu [17], and Philodendron birkin [10], but less information is available for the in vitro propagation of Philodendron erubescens cv. pink princess.…”

Optimizing factors influencing micropropagation of ‘Bluecrop’ and ‘Biloxi’ blueberries and evaluation of their morpho-physiological characteristics during ex vitro acclimatization

“…However, in recent years, the addition of nanomaterials has been shown to be a revolutionary alternative to optimize organogenesis protocols [13,14]. In plant tissue culture, the application of nanoparticles such as silver has been shown to be useful for eliminating microorganisms [15,16]. In addition, several studies have shown that the use of silver or cobalt nanoparticles can improve the regeneration and growth of explants, as well as the development and quality of seedlings [13,17,18], as they play an important role in inhibiting the formation and activity of ethylene gas [13,14].…”

Potencial ornamental e cultivo >i<in vitro>/i< de >i<Thaumatophyllum>/i< do Brasil

“…Já , realizou estudos com as variedades comerciais de Philodendron ('Imperial Green', 'Imperial Red' e 'Imperial Rainbow'), e para estes casos foram testados diferentes explantes, como partes do pecíolo foliar, lâmina foliar e segmento nodal do caule. Ascencio et al (2021), foram utilizados explantes de gemas axilares, testando diferentes tipos de assepsia, a fim de estabelecer em meio asséptico, sem contaminantes, exemplares de Philodendron xanadu (Thaumatophyllum xanadu). Henley et al, (2005) relataram que a maioria das plantas de Philodendron cultivadas a partir de estacas convencionais, produzem poucos brotos laterais, enquanto aquelas produzidas por cultura de tecidos possuem mais brotos basais, o que permite a produção de plantas mais compactas, mesmo em vasos pequenos.…”