Callus induction and bioactive phenolic compounds production fromByrsonima verbascifolia(L.) DC. (Malpighiaceae)

Abstract

-This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L -1 sucrose, solidified with 7 g L -1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 M) and BAP (0; 4.44; 8.88; 17.75 M ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and absence of light. We obtained friable and compact callus in yellow, green, and red. Culture media containing 4.52 M 2,4-D + 4.44 M BAP induced 100% of friable callus with higher fresh and dry weight in the absence of light. The callus produced higher amounts of total phenols and flavonoids than the initial explant.

Leaf and stem micromorphology of Byrsonima sericea DC. by light and scanning electron microscopy

“…The presence of phenolic compounds in the cortical parenchyma of the petiole and medullar parenchyma of the stem corroborate those described for other species of Byrsonima by Saldanha and Soares () and Castro, Braga, Sousa, Coimbra, and Chagas ().…”

“…The presence of sclerenchyma in the stem is another differential character for B. sericea, as Metcalfe and Chalk (1950) noted the absence of that tissue in the genera Byrsonima and Malpighia. Anatomical studies of the stems of Malpighiaceae taxa have been very infrequent, and only reported for Camarea (Mamede, 1993) and Stigmaphyllon those described for other species of Byrsonima by Saldanha and Soares (2015) and Castro, Braga, Sousa, Coimbra, and Chagas (2016).…”

Callus induction in three mosaic disease resistant cassava cultivars in Benin and genetic stability of the induced calli using simple sequence repeat (SSR) and sequence-characterized amplified region (SCAR) markers

“…The results of the current study were similar to that of Phua et al (2016) andFathil et al (2017), who reported that 2, 4-D at low (0.25 -1 mg/L) concentrations produced 100% callus in Clinacanthus nutans and in Citrus suhuiensis. Similarly, bp bp bp bp Castro et al (2016) reported in Byrsonima verbascifolia a maximal callus induction (100%) using 2, 4-D combined with BAP. The callus induced during the current study did not form embryos when subcultured on GA 3 supplemented medium and hormone free-medium.…”

“…Phua et al (2016) andFathil et al (2017) reported that 2,4-D at low (0.25 -1 mg/L) concentrations produced 100% callus in Clinacanthus nutans and Citrus suhuiensis. Furthermore, Castro et al (2016) reported in Byrsonima verbascifolia an excellent (100%) callus induction using 2,4-D combined with BAP. Callus-derived somaclonal variation based on morphological and biochemical parameters have been reported by Pajević et al (2004) in sunflower, Jibu et al (2006) in tea, Rajeswari et al (2009) in Sugarcane, Park et al (2010) in Rice, Beyene et al (2016) and Chauhan et al (2018) in cassava.…”

Antioxidant Activity, Rutin Content and Genetic Similarity Between Matrices and Progenies of Hancornia speciosa

“…The in vitro culture of plant cells presents advantages for the supply of bioactive compounds with defined production system and short culture periods, ensures a continuous supply of metabolites of interest, and the cells are free of diseases and are not exposed to seasonal variations (Castro et al, 2016). Manipulations of in vitro nutritional components and physical factors are the main factors for the culture productivity optimization (Fazal et al, 2016).…”