Leaf and stem micromorphology of <i>Byrsonima sericea</i> DC. by light and scanning electron microscopy

Silva, Anauara L E; Terceiro, Lucas E de Lima; Lima, Matheus Fabião de; Costa‐Silva, Rafael; Santos, Ednalva Alves Vital; Agra, Maria de Fátima

doi:10.1002/jemt.23412

Cited by 11 publications

(5 citation statements)

References 42 publications

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“…Stomata is a basic plant-specific organelle for transpiration and respiration, and Fe significantly affected the stomatal size and upper epidermis thickness of leaves [43,44]. Fe-deficiency caused a smaller stomatal size on the leaf surface, decreased the degree of stomatal opening, and affected the gas exchange of plants [45]. In this study, stomatal density increased with supplementary Fe treatments regardless of the Fe source in pH 5.70.…”

Section: Discussionmentioning

confidence: 50%

Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro

Xiao

Park

Guo

et al. 2020

IJMS

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Sorbus commixta is a valuable hardwood plant with a high economical value for its medicinal and ornamental qualities. The aim of this work was to investigate the effects of the iron (Fe) source and medium pH on the growth and development of S. commixta in vitro. The Fe sources used, including non-chelated iron sulfate (FeSO4), iron ethylenediaminetetraacetic acid (Fe-EDTA), and iron diethylenetriaminepentaacetic acid (Fe-DTPA), were supplemented to the Multipurpose medium with a final Fe concentration of 2.78 mg·L−1. The medium without any supplementary Fe was used as the control. The pH of the agar-solidified medium was adjusted to either 4.70, 5.70, or 6.70. The experiment was conducted in a culture room for six weeks with 25 °C day and night temperatures, and a 16-h photoperiod with a light intensity of 50 mmol·m−2·s−1 photosynthetic photon flux density (PPFD). Both the Fe source and pH affected the growth and development of the micropropagated plants in vitro. The leaves were greener in the pH 4.70 and 5.70 treatments. The tissue Fe content decreased with the increase of the medium pH. The leaf chlorophyll content was similar between plants treated with FeSO4 and those with Fe-EDTA. The numbers of the shoots and roots of plantlets treated with FeSO4 were 2.5 and 2 times greater than those of the control, respectively. The fresh and dry weights of the shoot and the root were the greatest for plants treated with Fe-EDTA combined with pH 5.70. The calcium, magnesium, and manganese contents in the plantlets increased in the pH 5.70 treatments regardless of the Fe source. Supplementary Fe decreased the activity of ferric chelate reductase. Overall, although the plantlets absorbed more Fe at pH 4.70, Fe-EDTA combined with pH 5.70 was found to be the best for the growth and development of S. commixta in vitro.

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Section: Discussionmentioning

confidence: 50%

Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro

Xiao

Park

Guo

et al. 2020

IJMS

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“…Anatomical characters are important for classifying and delimiting angiosperm species (Farias et al, 2016; Gomes et al, 2005; Lopes‐Silva et al, 2021; Metcalfe & Chalk, 1950). Microscopic techniques, including light (LM) and scanning electron microscopy (SEM) have provided fundamental information for delimiting different groups of plants (Shah et al, 2018; Silva et al, 2020; Ullah et al, 2019). Recent studies of Convolvulaceae have used plant anatomy to delimit species such as Argyreia Lour.…”

Section: Introductionmentioning

confidence: 99%

Leaf and stem micromorphology of Jacquemontia evolvuloides (Moric.) Meisn. (Convolvulaceae) populations: New insights for taxonomic classification using light and scanning electron microscopy

Belo

Buril

Santos

et al. 2023

Microscopy Res & Technique

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Morphoanatomical studies can provide useful and relevant information to support taxonomic groupings. Jacquemontia evolvuloides shows great morphological variability, which has led to numerous taxonomic classifications. To determine if anatomical characters can be used to recognize operational taxonomic units within populations of that species, we analyzed the leaves and stems of 22 populations using light and scanning electron microscopy. The variability of the analyzed characters allowed the grouping of these populations into five morphotypes. The presence of paracytic stomata, laticiferous canals, and stellate trichomes can be considered diagnostic characters of J. evolvuloides. The presence and types of epicuticular waxes, as well as a layer similar to palisade parenchyma in the petioles and stems, the classifications of glandular trichomes, and new types of stomata (anomocytic, anomotetracytic, and brachyparatetracytic) are reported here for the first time for Jacquemontia. The results discussed here help clarify the classification of this species complex and contribute to the taxonomy of Jacquemontia—a genus that has historically been difficult to define due to its wide morphological variation at the species level.Research Highlights Seven types of epicuticular waxes were identified among J. evolvuloides specimens: granules, threads, entire platelets, coiled rodlets, fissured layers, membranous platelets, and tubules. Six types of trichomes were observed among J. evolvuloides populations: stellate, malpighiaceous, sessile peltate glandular, short pedunculate glandular, stipitate‐glandular, and capitate glandular. We observed that six populations of Jacquemontia evolvuloides located in the Brazilian Caatinga domain have unprecedented sessile peltate trichomes restricted to the main leaf midrib, which were only observed under light microscopy.

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“…The identification and classification of plants, the determination of broader biotic/genetic diversity, and the characterization of reactions and resistance against stress factors are often based on investigations of morphological features, especially microscopic level analyses (Al‐Ashkar et al, 2020; Ayaz et al, 2021; Ayaz, Zaman, Saqib, Ullah, & Mahmood, 2020; Hu et al, 2020; Silva et al, 2020). Since gypsum‐based edaphic endemics often have substrate specific physiological mechanisms or strategies to cope with the harsh conditions that are characteristic the soil in which grow, these extreme features are expected to be reflected in the morphology and anatomy of gypsophytes.…”

Section: Introductionmentioning

confidence: 99%

Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

Kayabaş

2021

Microscopy Res & Technique

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Alyssum nezaketiae is a local endemic species in gypsum habitats of Çankırı province in Turkey. In this study, I used field collected specimens of A. nezaketiae in a scanning electron microscopy examination of vegetative and reproductive structures of A. nezaketiae. The objective of the study was to investigate the micromorphological properties that define the species and that represent its adaptation(s) to the extreme environmental conditions posed by gypsum habitats. The epidermis had one to two rows at transverse sections of leaf and stem. Cortex was composed six to eight rows parenchyma cells at stem. A number of adaptations to xeric habitats were observed, including: dense silvery lepidote hairs on leaves and stems, small leaves, stomata on the lower surfaces of the leaves occurring in stomatal crypts, intense lignification in the central cylinder of the stems, and bundles of sclerenchyma in the pith region of stems. These can be interpreted as gypsophytic characteristics associated with physiological drought. Micromorphological properties of plant parts are discussed in relation to environmental conditions and possible constraint factors occurring in these gypsum habitats. Furthermore, these micromorphological studies contribute important taxonomic characters that are needed to help identify a species whose habitat is under threat from a variety of human activities.

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Leaf and stem micromorphology of Byrsonima sericea DC. by light and scanning electron microscopy

Cited by 11 publications

References 42 publications

Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro

Effect of Iron Source and Medium pH on Growth and Development of Sorbus commixta In Vitro

Leaf and stem micromorphology of Jacquemontia evolvuloides (Moric.) Meisn. (Convolvulaceae) populations: New insights for taxonomic classification using light and scanning electron microscopy

Micromorphological considerations on Alyssum nezaketiae Aytaç & H. Duman (Brassicaceae), endemic to gypsum habitats from Turkey: An electron microscopic study

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