Genetic variability in early-cycle erect cowpea analysed with RAPD and ISSR markers

Dias, Francisco Tiago Cunha; Bertini, Cândida Hermínia Campos de Magalhães; Silva, Ana Paula Moura da; Cavalcanti, José Jaime Vasconcelos

doi:10.5935/1806-6690.20150039

Cited by 8 publications

(13 citation statements)

References 14 publications

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“…The size of the DNA fragments ranged from 100 to 1600 bp, values similar to those found for this species by Dias et al (2015), of 300 to 1400 bp, and for the genus Vigna by Ajibade et al (2000), of 200 to 1500 bp.…”

Section: Resultssupporting

confidence: 82%

“…When studying cowpea genotypes from Brazil and Nigeria using ISSR markers, Dias et al (2015) found 76% polymorphism, a high value and silimar to that found in our study; while Ghalmi et al (2010), studying local varieties of the species from Africa, found 63%, a value also considered high. The high values for polymorphism provided by ISSR primers reflect their coverage of the genome, since microsatellites, besides being abundant, are well distributed (Mahfouz, 2015).…”

Section: Resultssupporting

confidence: 70%

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Research Article Genetic diversity in cowpea landraces analyzed by ISSR markers

Araújo¹,

Fiege²,

Silva³

et al. 2019

Genet. Mol. Res.

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The cowpea, Vigna unguiculata (Fabeaceae) is widely cultivated in semi-arid regions, such as northeast Brazil. Due to the low crop yields in this region, it would be useful to develop cultivars adapted to these climate conditions. Landraces are seen as an important source of germplasm to be used in breeding programs of this species due to their good adaptation to the environment, but for this strategy to be viable, their genetic variability must be studied. To this end, we evaluated the genetic diversity, using ISSR molecular markers, of 52 samples of cowpea landraces collected mostly from small producers from all over the state of Ceará, Brazil. The DNA of the genotypes was extracted and analyzed using 25 primers. Based on the electrophoresis profiles of the bands, a genetic dissimilarity matrix was prepared, and a cluster analysis made using the UPGMA and modified Tocher methods. Fourteen primers amplified 80 bands, of which 61 were polymorphic, generating a polymorphism rate of 76%. The selected markers were efficient in identifying genetic variability among the varieties under evaluation, providing a large amount of information. The polymorphic information content varied from 0.13 to 0.66 and the band frequency ranged from 0.01 to 1.00. The two clustering methods agreed in the number of groups formed (n = 6), with the genetic distances ranging from 0.05 to 0.31, values considered low, suggesting a narrow genetic base for the landraces of this species in Ceará state.

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Section: Resultssupporting

confidence: 82%

Section: Resultssupporting

confidence: 70%

Research Article Genetic diversity in cowpea landraces analyzed by ISSR markers

Araújo¹,

Fiege²,

Silva³

et al. 2019

Genet. Mol. Res.

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“…These results are in agreement with Igwe et al (2017) who studied the genetic diversity among cowpea genotypes using SCoT and ISSR markers, they found that both marker types demonstrated high values for total number of alleles, genetic parameters (PIC, MI and RP), genetic diversity and total number of polymorphic bands. Dias et al (2015) and Araújo et al (2019) found 76% polymorphism, a high value and similar to that was found in our study; also Ghalmi et al (2010) studied the genetic diversity among 20 cultivars of cowpea using RAPD and ISSR markers, revealed relatively high levels of diversity which were similar to our results. Mahfouz (2015) studied the genetic diversity among cowpea genotypes and revealed that the high polymorphism attained by ISSR markers show their coverage of the genome.…”

Section: Fig 2b C (B)issr Profiles Of Six Cowpea Genotypes (C)supporting

confidence: 90%

Relationships between Hybrid Performance and Genetic Distance Revealed by Morphological and Molecular Markers in Cowpea (Vignaunguiculata (L.) Walp)

El-Fattah

Haridy

El-Rawy

2019

Journal of Agricultural Chemistry and Biotechnology

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The genetic diversity and relationships among six cowpea (Vigna unguiculata) genotypes were evaluated using 10 agromorphological traits and two molecular marker systems ISSR and SRAP. The phenotypic distance (PD) among all genotypes was relatively high. ISSR markers were more efficient than SRAP with regards to polymorphism detection, average number of polymorphicbands per primer (PB), resolving-power (RP), marker-index (MI) and polymorphism-information-content (PIC). ISSR and SRAP markers were generated cultivar or genotype specific unique DNA fingerprints able to identify the most diverse genotypes. The Dic genetic similarity ranged from 0.744 (P 1 and P 4 ) to 0.868 (P 2 and P 3 ). A positive correlation was found between ISSR and SRAP markers as well as between molecular markers and phenotypic markers. Based on phenotypic distance (PD) and genetic distance (GD), six parents of cowpea were crossed in half diallel fashion in order to determine combining ability to identify promising hybrids for ten traits including yield and its components. The both additive and non-additive effects of the controlling genes were involved in the inheritance of the traits studied. High broad-sense heritability estimates were obtained for all the traits as well as the narrow-sense heritability was larger than 0.60 in time to 50 % flowering, pod length, weight of pods per plant, weight of seeds per plant and total dry seed yield, so selection for these traits could be useful. The adequacy of additive-dominance model was fit for time to 50 % flowering, number of branches per plant, weight of pods per plant, weight of seeds per plant and total dry seed yield, while non-allelic gene interaction was observed for pod length, number of seeds per pod and pod diameter. The estimates of general combining effects revealed that P 5 had the highest positive and significant values for number of pods per plant, weight of pods per plant, weight of seeds per pod and total dry seed yield while P 1 exhibited the lowest negative and significant GCA for number of pods per plant, weight of pods per plant, weight of seeds per pod and total dry seed yield. The highly significant correlations were found between total dry seed yield and number of pods per plant (0.87), weight of pods per plant (0.95) and weight of seeds per plant (0.95). Mating designs used in this study were suitable for studying genetic parameters in cowpea. The high values of broad-sense and narrow-sense heritability indicated a good genetic variability for effective selection. The relationship between phenotypic and genotypic distance as well as the heterosis and SCA were estimated. Results indicated that the genetic distance was positive and/or negative and significantly correlated with some traits, while it was not significantly correlated with effects of heterosis and SCA for some other traits. Our results noted that knowledge about the genetic distance between parents can be used to predict hybrids performance.

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“…The molecular types present the particularity of use under analysis at any stage of plant development (Martuscello et al, 2015, Vieira et al, 2015. Among molecular markers, ISSRs (Inter Simple Sequence Repeat) stand out, because they do not require prior information of the DNA sequence and present laboratory procedures with good transferability (Dias et al, 2015). ISSR are semi-arbitrarily amplified by PCR (polymerase chain reaction), using a complementary primer for a designated microsatellite (Nilkanta et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers

Costa¹,

Fracetto²,

Fracetto³

et al. 2018

gmr

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Genetic diversity is the basis for genetic improvement, providing genes for new cultivars. Stylosanthes scabra is a legume used in pastures, presenting economic importance in tropical and subtropical regions. Molecular markers are targeted in population studies, thus we evaluated the genetic diversity distribution of S. scabra in Brazilian semi-arid northeastern among and within populations, from molecular markers ISSR. Four natural populations of S. scabra located in the municipalities of Santa Cruz do Capibaribe, Floresta, Sertânia and Petrolina from Pernambuco state were used. We selected seven ISSR primers for amplification and analyzed 75 individuals. A total of 88 bands were amplified with a percentage of 95.27% polymorphism at the species level. AMOVA test revealed that 40.0% of the total genetic variation is within populations while 60.0% among populations. The population differentiation was 0.332 and the migrant's number per generation was 0.5. Grouping analysis confirmed a high level of differentiation among populations. The populations with the greatest variability were Santa Cruz do Capibaribe and Petrolina, and the ISSR markers were efficient for the genetic diversity quantification in S. scabra, which presented greater variability among populations than Lira Júnior MA, et al 2 Genetics and Molecular Research 17 (1): gmr16039866 within populations. In short, this variability will be very useful in future breeding programs for this species.

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Genetic variability in early-cycle erect cowpea analysed with RAPD and ISSR markers

Cited by 8 publications

References 14 publications

Research Article Genetic diversity in cowpea landraces analyzed by ISSR markers

Research Article Genetic diversity in cowpea landraces analyzed by ISSR markers

Relationships between Hybrid Performance and Genetic Distance Revealed by Morphological and Molecular Markers in Cowpea (Vignaunguiculata (L.) Walp)

Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers

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