The
Ralstonia spp
. genus is a group of non-fermentative, Gram-negative bacteria often resistant to many antibiotics, which are emerging as opportunistic pathogens frequently associated with infections in hospital settings. We present herein a case of combined
R. pickettii
and
R. mannitolilytica
persisting and relapsing bacteraemia, possibly caused by a septic arterial thrombosis secondary to the rupture of an internal carotid artery aneurysm. Microbiology studies showed that both
Ralstonia
isolates produced biofilm and carried class D oxacillinase genes. When confronted with infections caused by members of the
Ralstonia
genus, identification to the species level is crucial for correct clinical management, as the two species show different antibiotic susceptibility patterns.