Effect of Organic Solvent Composition on Dissociation Constants of Some Reversible Acetylcholinesterase Inhibitors

DALDAL, Yaşar Doğan; Demıralay, Ebru; Özkan, Síbel A.

doi:10.5935/0103-5053.20150276

Cited by 6 publications

(5 citation statements)

References 1 publication

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“…values depend on the ACN composition [30] and the values of MCZ and BCZ were determined from the combined effect of pH of the mobile phase and experimental tR values. The sigmoidal behavior was observed when the tR values of MCZ and BCZ measured in the pH 5.0-9.0 range were plotted (Figure 3).…”

Section: Determination Of Pka Valuesmentioning

confidence: 99%

Thermodynamic protonation constant values of meclizine and buclizine in acetonitrile-water binary mixtures

Nane¹,

Demıralay²

2021

jrp

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In the study, the retention times (tR) values for meclizine (MCZ) and buclizine (BCZ) structurally related benzhydrylpiperazine were determined to modeling the dependence of retention on the acetonitrile concentration in the mobile phase. Reversed-phase liquid chromatography (RPLC) analysis of the MCZ and BCZ was realized with polar embedded amide (15x0.46 cm I.D, 5μm) column. The mobile phase pH range is selected from 5.0-9.0 to determine the structural changes due to protonation that result from a change in pH. The different retention time were attributed to their different hydrophobicities and ionization degree. The thermodynamic protonation constant () value for investigated compounds was calculated using the tR relationship to pH of the mobile phase and effect of the activity coefficients. Linear correlation between values and macroscopic parameters (XACN, 1/ε) was also determined.

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Section: Determination Of Pka Valuesmentioning

confidence: 99%

Thermodynamic protonation constant values of meclizine and buclizine in acetonitrile-water binary mixtures

Nane¹,

Demıralay²

2021

jrp

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“…There are studies in the literature for the determination of sertraline, citalopram, and fluvoxamine alone or together with their degradation products in various samples [6][7][8]. Among these methods, reverse phase liquid chromatography (RPLC) is mostly preferred due to its advantages such as accuracy, precision, and repeatability of the measurements taken [9][10][11][12]. The primary purpose of RPLC studies is to ensure that the studied compounds are separated from each other as soon as possible or to make simultaneous determinations provided that certain validation conditions (ICH parameters) are met [13].…”

Section: Introductionmentioning

confidence: 99%

RPLC Method Development and Validation for the Simultaneous Determination of Selective Serotonin Reuptake Inhibitors in Tablet Formulations

Demıralay

ÜRER

Üstün

et al. 2023

Ankara Ecz. Fak. Derg.

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Objective: Selective serotonin reuptake inhibitor (SSRIs) compounds are the most used compounds in the treatment of depression. The determination of chromatographic separation and quantitative determination of these compounds is very important in the clinical use of these compounds and the success of biopharmaceutical studies. For this reason, this study, it was aimed to optimize the chromatographic conditions for the quantitative determination of SSRIs in tablet formulation by Reversed phase liquid chromatography method. Material and Method: The optimum separation condition for the studied compounds was determined based on the relationship between the retention values of the compounds and the pH and the content of the mobile phase. Chromatographic determination was made on the X Terra C18 column (250 x 4.6 mm I.D., 5 μm), which is widely used for the determination of hybrid-based and basic compounds. The validation of the developed method was carried out based on the parameters of linearity, precision, and accuracy. Result and Discussion: The developed and validated method was successfully applied for the determination of active ingredients in the tablet dosage form. The experimental results of the amount of studied SSRIs in selected commercial tablets are in good agreement with the label claims. The calculated percent recoveries show that the sample preparation techniques developed for the quantification of the compounds studied are not affected by interferences. The evaluation of the obtained results showed that the developed method is suitable for the routine use of studied compounds.

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“…Capillary electrophoresis (CE), high performance liquid chromatography (HPLC), and electrochemical methods are widely used for the analysis of compounds in drug formulations and biological fluids [6]. Among these methods, reverse phase liquid chromatography (RPLC) is more preferred due to its advantages such as accuracy, precision, and reproducibility of the measurements [7][8][9][10]. The primary purpose of RPLC studies is to ensure that the studied compounds are separated from each other as soon as possible or to make simultaneous determinations provided that certain validation conditions ICH parameters are met [11,12].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of RPLC method for the simultaneous analysis of ACE inhibitors in tablet formulations

KUZUCANLI

Demıralay

DALDAL

et al. 2022

Turkish Journal of Analytical Chemistry

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Hypertension is a disease that can progress for many years without symptoms or complications. This disease can cause death and permanent damage to vital organs such as the kidney, heart, and brain. However, when hypertension is treated effectively, these risk factors may decrease significantly. Although many antihypertensive drugs are used in the treatment of hypertension, ACE inhibitors are a new class among these drugs, and their separation and quantitative determinations are important for the success of preclinical, biopharmaceutical, and clinical studies. For this reason, in this study, the simultaneous determination of cilazapril, benazepril, and quinapril from ACE inhibitors, the validation of the developed method, and the quantitative determination of commercial drug dosage forms are aimed. To obtain the optimum liquid chromatographic separation condition, a systematic approach based on the relationship between mobile phase pH and retention time was used. The mobile phase organic modified concentration and column temperature parameters, which are known to affect the retention times of the compounds, were kept constant. When deciding on these parameters, few experiments were carried out.

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Effect of Organic Solvent Composition on Dissociation Constants of Some Reversible Acetylcholinesterase Inhibitors

Cited by 6 publications

References 1 publication

Thermodynamic protonation constant values of meclizine and buclizine in acetonitrile-water binary mixtures

Thermodynamic protonation constant values of meclizine and buclizine in acetonitrile-water binary mixtures

RPLC Method Development and Validation for the Simultaneous Determination of Selective Serotonin Reuptake Inhibitors in Tablet Formulations

Development and validation of RPLC method for the simultaneous analysis of ACE inhibitors in tablet formulations

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