Application of a Quantitative HPLC-ESI-MS/MS Method for Flavonoids in Different Vegetables Matrices

Perlatti, Bruno; Fernandes, João B.; Silva, Maria Fátima das Graças Fernandes da; Ardila, Jorge Armando; Carneiro, Renato Lajarim; Souza, Bruno Henrique Sardinha de; Costa, Eduardo Neves; Eduardo, Wellington Ivo; Boiça, Arlindo Leal; Forim, Moacir Rossi

doi:10.5935/0103-5053.20150273

Cited by 12 publications

(10 citation statements)

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“…The content of: ampelopsin, apigenin, daidzein, genistein, glycitein, hesperetin, hesperidin, isorhamnetin, kaempferol, luteolin, naryngin, quercetin, rutin, taxifolin was determined. The selection of the flavonoid spectrum for analysis was based on literature data 86 – 89 .…”

Section: Methodsmentioning

confidence: 99%

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

Stec

Kordan

Sergiel

et al. 2021

Sci Rep

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To reveal the antixenosis potential against the pea aphid Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) we analyzed the pea aphid survival and probing behavior, and the quantitative and qualitative variation of flavonoids in the leaves of selected soybean Glycine max (L.) Merr (Fabaceae) cultivars ‘Aldana’, ‘Annushka’, ‘Augusta’, ‘Madlen’, ‘Mavka’, ‘Simona’, ‘Violetta’, and ‘Viorica’. Aphid survival was drastically impeded on all cultivars. The electronic monitoring of aphid probing using the Electrical Penetration Graph (EPG) technique revealed that on all soybean cultivars, A. pisum readily probed into leaf tissues but the probes were usually terminated before reaching vascular tissues, which demonstrates the activity of antixenosis mechanisms in peripheral tissues epidermis and/or mesophyll in soybean leaves. The potency of antixenosis factors differed among soybean cultivars, which was reflected in differences in aphid survival and frequency and duration of phloem sap ingestion. Seven flavonoids were found: apigenin, daidzein, genistein, glycitein, isorhamnetin, kaempferol, and rutin, which occurred in different amount and proportion in individual cultivars. The content of apigenin and genistein in all soybean cultivars studied probably made them relatively unacceptable to A. pisum. Kaempferol in ‘Aldana’ might be responsible for the observed strong antixenosis resistance of this cultivar to A. pisum. The results of our survey provide the first detailed data that can be used for future studies.

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Section: Methodsmentioning

confidence: 99%

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

Stec

Kordan

Sergiel

et al. 2021

Sci Rep

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“…Nitrogen was used as the carrier, heater, and collision gases. The Applied Biosystems Analyst version 1.5.2 software was used to control for data acquisition and analysis [20].…”

Section: Methodsmentioning

confidence: 99%

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice

Shang

et al. 2018

Oxidative Medicine and Cellular Longevity

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Aging is a major factor that contributes to neurological impairment and neuropathological changes, such as inflammation, oxidative stress, neuronal apoptosis, and synaptic dysfunction. Flavonoids act as protective antioxidant and anti-inflammatory agents against various age-related neurodegenerative diseases. Here, we investigated the protective effect and mechanisms of the flavonoid-rich ethanol extract from the leaves of Diospyros kaki (FELDK) in the cortex and hippocampus of D-galactose- (gal-) aged mice. Our results showed that FELDK treatment restored memory impairment in mice as determined by the Y-maze and Morris water maze tests. FELDK decreased oxidative stress levels via inhibiting reactive oxygen species (ROS) and malondialdehyde (MDA) production and elevating antioxidative enzymes. FELDK also alleviated D-gal-induced neuroinflammation via suppressing the expression of advanced glycation end products (AGEs) and receptor for AGEs (RAGE) and activating microgliosis and astrocytosis, nuclear factor kappa B (NF-κB) nuclear translocation, and downstream inflammatory mediators. Moreover, FELDK inhibited the phosphatidylinositol 3-kinase (PI3K)/Akt and C-jun N-terminal kinase (JNK) apoptotic signaling pathways and ameliorated the impairment of synapse-related proteins. Hence, these results indicate that FELDK exerts neuroprotective effects on D-gal-induced brain aging. Thus, FELDK may be a potential therapeutic strategy for preventing and treating age-related neurodegenerative diseases such as Alzheimer's disease.

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“…Quantitative analysis of avonoids was performed in the Department of Chemistry of the Federal University of São Carlos, in São Carlos, state of São Paulo, Brazil. The avonoids rutin, isoquercitrin, daidzin, daidzein, hesperidin, naringin, naringenin, and hesperidin in younger and older leaves of the resistant and susceptible soybeans at vegetative and reproductive stages were quanti ed using high performance liquid chromatography -mass spectrometry (HPLC-MS) following the methodology developed by Perlatti et al (2016).…”

Section: Concentrations Of Nutrients and Avonoids In Younger And Older Leaves Of Soybeans At Vegetative And Reproductive Stagesmentioning

confidence: 99%

Soybean leaf age and plant stage influence expression of resistance to velvetbean caterpillar and fall armyworm

et al. 2021

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Numerous species of herbivorous insects are associated with soybeans, including the specialist velvetbean caterpillar (VBC), Anticarsia gemmatalis, and the generalist fall armyworm (FAW), Spodoptera frugiperda. Expression of plant resistance is in uenced by factors intrinsic to host plants, such as leaf age and plant stage, which can differentially affect specialist and generalist insects due to varying levels of plant defense and corresponding insect adaptation. In this study four experiments were carried out to test the hypotheses that levels of antibiosisresistance to VBC and FAW in the resistant genotype PI 227687 and susceptible genotype IGRA RA 626 RR are related to leaf age and plant stage of soybean. Furthermore, the concentrations of nutrients and selected avonoids were quanti ed to give insights on possible chemical mechanisms underlying the resistance. As results, development of VBC and FAW were negatively affected when larvae fed leaves of the resistant genotype, older leaves from the lower part of plants, or leaves from reproductive-stage soybeans. The effects were partly different for each insect species, and the generalist FAW was more affected by higher resistance levels in the older leaves of soybean than the specialist VBC. Distribution and concentrations of nutrients and avonoids in soybean in function of leaf age and plant stage may explain the varying levels of antibiosis-resistance to VBC and FAW. These results can bene t developments of speci c protocols for screening resistant soybean genotypes and pest management strategies focused in plant parts and growth stages that insect-resistance levels are lowest. Key MessageLeaf age and plant stage are factors that in uence expression of resistance to insects. These factors were investigated in soybean genotypes on VBC and FAW development.Development of VBC and FAW were negatively affected on the resistant genotype, older leaves, and leaves from reproductive-stage soybeans, and the varying levels of resistance may be explained by avonoids and nutrients concentrations.The results can bene t developments of speci c protocols for screening soybean resistant genotypes and pest management strategies.

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Application of a Quantitative HPLC-ESI-MS/MS Method for Flavonoids in Different Vegetables Matrices

Cited by 12 publications

References 0 publications

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

Antixenosis in Glycine max (L.) Merr against Acyrthosiphon pisum (Harris)

Flavonoid-Rich Ethanol Extract from the Leaves of Diospyros kaki Attenuates D-Galactose-Induced Oxidative Stress and Neuroinflammation-Mediated Brain Aging in Mice

Soybean leaf age and plant stage influence expression of resistance to velvetbean caterpillar and fall armyworm

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