Simultaneous Determination of Catecholamines and Metanephrines in Urine by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry: Successful Clinical Application

Diniz, Maria E. R.; Vilhena, Leandro Schiavo; Paulo, Breno P.; Barbosa, Tatiana C. C.; Mateo, Elvis Cueva

doi:10.5935/0103-5053.20150142

Cited by 6 publications

(18 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Available assays often suffered low sensitivity for detecting trace amount of E [19,21,25,26] and narrow linearity ranges for monitoring high levels of DA and metanephrines [23]. Consequently, additional time and cost were needed to quantify these analytes.…”

Section: 2ms Condition Optimizationmentioning

confidence: 99%

“…Available sample preparation involving an essential pH adjustment made the process cumbersome and time-consuming [19][20][21][22][23][24][25][26]32]. This was attributed to the substantial pH variation in urine and large sample volume (typically over 500 μL).…”

Section: Effect Of Sample Volumementioning

confidence: 99%

“…An effective sample cleanup for the complex urine matrix prior to analysis is crucial. Liquid-liquid extraction [19,25] and solid phase extraction (SPE) [17,[20][21][22][23][24] have become the popular choices among other techniques [12,30]. It is noteworthy that the pH of the pre-treated urine sample was crucial for extraction recovery in the reported assays.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, to achieve a reliable extraction, it was cumbersome to adjust pH of each sample to a narrow pH range. For example, a liquid-liquid extraction needed a pH of 9.5 prior to LC-MS/MS analysis [19,25]; SPE using Oasis WCX, the most common cleanup of these analytes, required a narrow pH range of 6-7 [23,26,32].…”

Section: Introductionmentioning

confidence: 99%

“…Due to difficult collection of tissues, and supine rest constraint of blood draw for plasma, urine has been recommended as an alternative specimen for the analysis owing to its non-invasive and convenient collection [10,[14][15][16]. Among a wide range of methodologies with different detectors applied for the measurement of urinary catecholamines and metanephrines [6,12,17,18], liquid chromatography-tandem mass spectrometry (LC-MS/MS) has gradually taken the center stage due to its inherent selectivity and sensitivity [19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Pre-analytical and analytical validations and clinical applications of a miniaturized, simple and cost-effective solid phase extraction combined with LC-MS/MS for the simultaneous determination of catecholamines and metanephrines in spot urine samples

Li¹,

Li²,

Kellermann³

2016

Talanta

View full text Add to dashboard Cite

Section: 2ms Condition Optimizationmentioning

confidence: 99%

Section: Effect Of Sample Volumementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Pre-analytical and analytical validations and clinical applications of a miniaturized, simple and cost-effective solid phase extraction combined with LC-MS/MS for the simultaneous determination of catecholamines and metanephrines in spot urine samples

Li¹,

Li²,

Kellermann³

2016

Talanta

View full text Add to dashboard Cite

Novel LC-MS/MS Method for Simultaneous Determination of Monoamine Neurotransmitters and Metabolites in Human Samples

Kumar,

Sinha,

Gouda

2024

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

For the simultaneous determination of monoamine neurotransmitters (NTs) like dopamine, serotonin, noradrenaline, and epinephrine, and their metabolites (metanephrine, normetanephrine, 3-methoxytyramine, vanillylmandelic acid, 3,4-dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindoleacetic acid), a robust liquid chromatography method coupled with tandem mass spectrometry (LC–MS/MS) was introduced as the analytical method. This analytical method proved to be accurate for the simultaneous measurement of the amounts of 11 NTs and their metabolites in biological samples. The method proved to be more efficient and better than the previously reported method in terms of precision, recovery, sample requirement, and extraction procedure. The reported method requires only 100 μL of blood and 200 μL of urine, and the extraction procedure requires acetonitrile precipitation, filtration, drying, and reconstitution in water. The separation of all analytes was performed on an C18 column (4.6 mm × 150 mm and 1.8 μm). A 10 min gradient elution program with a mobile phase consisting of phase A (0.2% formic acid in water) and phase B (methanol) was used. The positive ionization mode was used for the detection of all analytes in multiple reaction monitoring (MRM). The proposed method was validated with an internal standard and yielded lower limits of detection and quantification ranges of 0.0182–0.0797 ng/mL and 0.0553–0.2415 ng/mL, respectively, with a good linearity (R 2) between 0.9959 and 0.9994. The recoveries ranged from 73.37% to 116.63% in blood and from 80.9% to 115.33% in urine. For the NTs and metabolites, the intra- and interday % CV were 0.24–9.36 and 0.85–9.67, respectively. The developed LC–MS/MS method was successfully used for the determination of trace amounts of endogenous compounds in human blood and urine samples.

show abstract

A Novel, Automated Dispersive Pipette Extraction Technology Greatly Simplifies Catecholamine Sample Preparation for Downstream LC-MS/MS Analysis

Mastrianni¹,

Kemnitzer²,

Miller³

2019

SLAS Technology

View full text Add to dashboard Cite

Catecholamines are integral neurotransmitters in the central and peripheral nervous system. Clinically, catecholamine levels are determined to help diagnose disease and measure corresponding therapeutic effectiveness. However, manual extraction of catecholamines and their metabolites may be labor-intensive and user-variable and require a variety of peripheral laboratory devices, especially at low sample concentrations. Here, we propose a novel solid-phase extraction (SPE) method using patented dispersive pipette extraction (DPX) tip technology. The tips are readily integrated into an automated workflow to extract these compounds from urine, which increases analytical throughput while removing human variability and error. Diphenylboronic acid (DPBA) forms a stable, negatively charged complex with catecholamines in the samples, and when aspirated into the DPX tip, the complexed analytes are retained on a styrene divinyl benzene sorbent. Wash buffers remove interfering compounds, after which the complex is eluted from the tip using an acidic aqueous solution and subsequently measured via liquid chromatography with tandem mass spectrometry (LC-MS/MS). The automated DPX method for catecholamine sample preparation from urine has excellent linearity over more than three orders of magnitude with concentrations ranging from 0.5 to 1000 ng/mL, with replicate analyses resulting in coefficients of variation of less than 8%. This high-throughput workflow is appropriate for use in regulated laboratories.

show abstract

Simultaneous Determination of Catecholamines and Metanephrines in Urine by Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry: Successful Clinical Application

Cited by 6 publications

References 0 publications

Pre-analytical and analytical validations and clinical applications of a miniaturized, simple and cost-effective solid phase extraction combined with LC-MS/MS for the simultaneous determination of catecholamines and metanephrines in spot urine samples

Pre-analytical and analytical validations and clinical applications of a miniaturized, simple and cost-effective solid phase extraction combined with LC-MS/MS for the simultaneous determination of catecholamines and metanephrines in spot urine samples

Novel LC-MS/MS Method for Simultaneous Determination of Monoamine Neurotransmitters and Metabolites in Human Samples

A Novel, Automated Dispersive Pipette Extraction Technology Greatly Simplifies Catecholamine Sample Preparation for Downstream LC-MS/MS Analysis

Contact Info

Product

Resources

About