Separation, Concentration and Determination of Trace Thiamphenicol in Egg, Milk and Honey Using Polyoxyethylene Lauryl Ether-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography

Yao, Hui; Lü, Yang; Han, Juan; Wang, Yun; Yan, Yongsheng

doi:10.5935/0103-5053.20150072

Cited by 3 publications

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“…Aqueous two‐phase system (ATPS) is an environmental extraction technology, which has been widely used to the separation and extraction of nucleic acids, proteins, enzymes, drugs, antibiotics, and other biological materials . ATPS has some advantages, such as short processing time, gentle biocompatible environment, and no use of toxic volatile organic solvent in the experiment.…”

Section: Introductionmentioning

confidence: 99%

“…6 Aqueous two-phase system (ATPS) 7,8 is an environmental extraction technology, which has been widely used to the separation and extraction of nucleic acids, 9 proteins, [10][11][12] enzymes, 13 drugs, 14 antibiotics, [15][16][17] and other biological materials. 18,19 ATPS has some advantages, such as short processing time, gentle biocompatible environment, and no use of toxic volatile organic solvent in the experiment. Usual types of ATPS include two different polymers ATPS, 20,21 polymer-salt ATPS, 22,23 ionic liquid (IL)-salt ATPS, 24 and small molecular watermiscible organic solvent-salt ATPS.…”

Section: Introductionmentioning

confidence: 99%

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Separation and determination of tetracycline hydrochloride in real water samples using binary small molecule alcohol‐salt aqueous two‐phase system coupled with high‐performance liquid chromatography

Donggang

Wang

et al. 2019

Chirality

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An environmental and sensitive sample pretreatment method was established and combined with high‐performance liquid chromatography (HPLC) for the analysis of tetracycline hydrochloride (TC) in feed water and lake water. One element small molecule alcohol‐salt aqueous two‐phase system (ATPS) cannot effectively adjust the polarity of the system, but binary small molecule alcohol‐salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics. In this work, a binary ATPS based on ethanol +2‐propanol + (NH4)2SO4 system was formed and applied to the separation and purification of TC in real water samples. The influence factors on partition behaviors of TC were discussed, including the types and the concentration of phase salts, the volume ratio of alcohol, the pH value, extraction temperature, and the standing time. The response surface methodology was used to determine the best experimental conditions for multi‐factor experiments. Under this optimal condition, the extraction efficiency of TC reached 95.7%. This new method is considered to have significant application in the divorce of antibiotics.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Separation and determination of tetracycline hydrochloride in real water samples using binary small molecule alcohol‐salt aqueous two‐phase system coupled with high‐performance liquid chromatography

Donggang

Wang

et al. 2019

Chirality

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“…Polyoxyethylene n -alkyl ether contained both hydrophobic group hydrophilic group, namely, alkyl and polyoxyethylene, so it was an appropriate class of polymer used with salts to form ATPS. As a part of polyoxyethylene n -alkyl ether, polyoxyethylene(10) lauryl ether (C 32 H 66 O 11 , POELE10) has been used as a composition of ATPS, and the phase diagram data of the ATPSs containing POELE10 and inorganic salts ((NH 4 ) 2 HPO 4 , KOH, K 2 HPO 4 , K 2 CO 3 , K 3 PO 4 , Li 2 SO 4 , MgSO 4 and ZnSO 4 )/organic salts (KC 6 H 11 O 7 , K 2 C 2 O 4 , K 2 C 4 H 4 O 6 , K 3 C 6 H 5 O 7 , (NH 4 ) 2 C 4 H 4 O 6 , and Na 2 C 4 H 4 O 6 ) at different temperatures have been determined. − Based on the above studies on phase diagram of POELE10–salts ATPS, the POELE10 ATPSs combined with high-performance liquid chromatography was used to separate/enrich/determine the trace antibiotic residues in food samples, , which have a satisfactory effect in the application. Therefore, we have built the ATPS composed of polyoxyethylene(20) cetyl ether (C 56 H 114 O 21 , POELE20) and three kinds of potassium salt (KOH, K 2 CO 3 , and K 3 PO 4 ) and have reported their liquid–liquid equilibrium composition .…”

Section: Introductionmentioning

confidence: 99%

“…14−18 Based on the above studies on phase diagram of POELE10−salts ATPS, the POELE10 ATPSs combined with high-performance liquid chromatography was used to separate/ enrich/determine the trace antibiotic residues in food samples, 19,20 which have a satisfactory effect in the application. Therefore, we have built the ATPS composed of polyoxyethylene (20) cetyl ether (C 56 H 114 O 21 , POELE20) and three kinds of potassium salt (KOH, K 2 CO 3 , and K 3 PO 4 ) and have reported their liquid−liquid equilibrium composition. 21 In this work, the phase diagram of ATPSs consisting of POELE20 and three kinds of sulfate salts (Li 2 SO 4 , ZnSO 4 , and MgSO 4 ) will be given, and the influence of factors (system temperatures and salts type) on the liquid−liquid equilibria composition of the investigated ATPSs will be discussed.…”

Section: Introductionmentioning

confidence: 99%

Phase Equilibria and Salt Effect on the Aqueous Two-Phase System of Polyoxyethylene Cetyl Ether and Sulfate Salt at Three Temperatures

Tan

et al. 2016

J. Chem. Eng. Data

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Polyoxyethylene(20) cetyl ether (C 56 H 114 O 21 , POELE20) and three sulfate salts (Li 2 SO 4 , ZnSO 4 , and MgSO 4 ) were used as phaseforming material of the aqueous two-phase system, and the binodal data was measured at the three temperatures (288.15, 298.15, 308.15 K). Three classic experienced formulas were used to fit the binodal data, and they had better performance in data association. The tie-line data of studied systems were measured and fitted by the empirical formulas, i.e., Othmer−Tobias and Bancroft equations. It has better performance in data association. For the investigated systems, the influence of the salt type on the binodal curves was studied by the Gibbs free energy of ions hydration and effective excluded volume, and the influence of temperature on the tie-lines and binonal curves was also discussed. First, as it is confirmed, when the salts are of the same anion, salts with higher cation valence will have stronger abilities of salting out; the salting out abilities of salts strengthen with the increasing absolute value of Gibbs free energies of cations. Second, the salting-out abilities of salts enhance with the increase in system effective excluded volume in the investigated systems. Third, the studied aqueous two-phase systems are more prone to formation at the higher temperature than those at lower temperature. Finally, for the tie-lines in the studied systems, the absolute value of slope decreases with the temperature falling.

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Simultaneous Prediction, Determination, and Extraction of Four Polycyclic Aromatic Hydrocarbons in the Environment Using a UCON–NaH2PO4 Aqueous Two-Phase Extraction System Combined with High-Performance Liquid Chromatography-Ultraviolet Detection

Chang

Sun

2022

Molecules

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In this paper, a new aqueous two-phase extraction system(ATPES) consisting of UCON (poly(ethylene glycol-ran-propylene glycol) monobutyl ether)–NaH2PO4 was established, and four trace polycyclic aromatic hydrocarbons (PAHs: fluorene, anthracene, pyrene and phenanthrene) in water and soil were analyzed by high-performance liquid chromatography (HPLC)–ultraviolet detection. In the multi-factor experiment, the central composite design (CCD) was used to determine the optimum technological conditions. The final optimal conditions were as follows: the concentration of UCON was 0.45 g·mL−1, the concentration of NaH2PO4 was 3.5 mol·L−1, and the temperature was 30 °C. The recovery of the four targets was 98.91–99.84% with a relative standard deviation of 0.3–2.1%. Then UCON recycling and cyclic tests were designed in the experiment, and the results showed that the recovery of PAHs gradually increased in the three extractions because of the remaining PAHs in the salt phase of last extraction. The recovery of PAHs in the UCON recycling test was less than that in the extraction test due to the wastage of UCON. In addition, a two-phase aqueous extraction model was established based on the random forest (RF) model. The results obtained were compared with the experimental data, and the root mean square error (RMSE) was 0.0371–0.0514 and the correlation coefficient R2 was 96.20–98.53%, proving that the model is robust and reliable.

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Separation, Concentration and Determination of Trace Thiamphenicol in Egg, Milk and Honey Using Polyoxyethylene Lauryl Ether-Salt Aqueous Two-Phase System Coupled with High Performance Liquid Chromatography

Cited by 3 publications

References 0 publications

Separation and determination of tetracycline hydrochloride in real water samples using binary small molecule alcohol‐salt aqueous two‐phase system coupled with high‐performance liquid chromatography

Separation and determination of tetracycline hydrochloride in real water samples using binary small molecule alcohol‐salt aqueous two‐phase system coupled with high‐performance liquid chromatography

Phase Equilibria and Salt Effect on the Aqueous Two-Phase System of Polyoxyethylene Cetyl Ether and Sulfate Salt at Three Temperatures

Simultaneous Prediction, Determination, and Extraction of Four Polycyclic Aromatic Hydrocarbons in the Environment Using a UCON–NaH2PO4 Aqueous Two-Phase Extraction System Combined with High-Performance Liquid Chromatography-Ultraviolet Detection

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