HPLC-DAD and UV-Vis Spectrophotometric Methods for Methotrexate Assay in Different Biodegradable Microparticles

Oliveira, Anderson Rodrigues de; Caland, Lilia Basílio de; Oliveira, Edilene Gadelha de; Egito, Eryvaldo Sócrates Tabosa do; Pedrosa, Matheus F. F.; Júnior, Arnóbio A. Silva

doi:10.5935/0103-5053.20150022

Cited by 12 publications

(12 citation statements)

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“…The drug content in the prepared GNPs was determined indirectly by analyzing the amount of free drug remaining in the supernatant after centrifugation of the loaded NPs according to a previously described high performance liquid chromatography (HPLC) validated method (Oliveira et al, 2015). Briefly, an HPLC instrument equipped with UV-visible detector was set to 303 nm.…”

Section: Determination Of Drug Loadingmentioning

confidence: 99%

Exploiting gelatin nanocarriers in the pulmonary delivery of methotrexate for lung cancer therapy

Abdelrady

Hathout

Osman

et al. 2019

European Journal of Pharmaceutical Sciences

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Section: Determination Of Drug Loadingmentioning

confidence: 99%

Exploiting gelatin nanocarriers in the pulmonary delivery of methotrexate for lung cancer therapy

Abdelrady

Hathout

Osman

et al. 2019

European Journal of Pharmaceutical Sciences

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“…Statistical calculations of MTX loaded on biodegradable microparticles with HPLC and UV–visible spectrophotometric techniques by Oliveira et al. showed that both methods provide similar results, and also can be used for the quality control of MTX in drug delivery systems [80]. Determination of MTX in rat plasma with HPLC-UV was done by Raichur and Devi [81].…”

Section: High Performance Liquid Chromatography Techniquementioning

confidence: 99%

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples

Karami

Ranjbar

Ghasemi

et al. 2019

Journal of Pharmaceutical Analysis

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Methotrexate (MTX) is a folate antagonist drug used for several diseases, such as cancers, various malignancies, rheumatoid arthritis (RA) and inflammatory bowel disease. Due to its structural features, including the presence of two carboxylic acid groups and its low native fluorescence, there are some challenges to develop analytical methods for its determination. MTX is metabolized to 7-hydroxymethotrexate (7-OH-MTX), 2,4-diamino-N10-methylpteroic acid (DAMPA), and the active MTX polyglutamates (MTXPGs) in the liver, intestine, and red blood cells (RBCs), respectively. Additionally, the drug has a narrow therapeutic range; hence, its therapeutic drug monitoring (TDM) is necessary to regulate the pharmacokinetics of the drug and to decrease the risk of toxicity. Due to environmental toxicity of MTX; its sensitive, fast and low cost determination in workplace environments is of great interest. A large number of methodologies including high performance liquid chromatography equipped with UV–visible, fluorescence, or electrochemical detection, liquid chromatography-mass spectroscopy, capillary electrophoresis, UV–visible spectrophotometry, and electrochemical methods have been developed for the quantitation of MTX and its metabolites in pharmaceutical, biological, and environmental samples. This paper will attempt to review several published methodologies and the instrumental conditions, which have been applied to measure MTX and its metabolites within the last decade.

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“…Therefore, TEF and MTX combination therapy suggests a possible alternative for those patients who fail MTX monotherapy having persistent active RA (Soneji, Shah, & Maheswari, 2015). A simple robust HPLC analytical method has been reported for the measurement of MTX in different dosage forms like tablets (Jogi, Rao, & Raju, 2016;Roy, Mohite, & Shah, 2016), microparticles (Oliveira et al, 2015), gels, injections, lipid nanocarriers and lipid polymer hybrid nanoparticles (Garg, Sharma, Singh, Nirbhavane, & Katare, 2015). Moreover, to calculate and optimize the dose of prepared nanoformulations, it is necessary to quantify the amount of each drug present in the nanoformulations; therefore, we have developed a high-performance liquid chromatography (HPLC) based analytical method for the simultaneous determination of MTX and TEF.…”

Section: Introductionmentioning

confidence: 99%

“…Both of the drugs have been analyzed separately and in combination with other drugs through various methods. A simple robust HPLC analytical method has been reported for the measurement of MTX in different dosage forms like tablets (Jogi, Rao, & Raju, 2016;Roy, Mohite, & Shah, 2016), microparticles (Oliveira et al, 2015), gels, injections, lipid nanocarriers and lipid polymer hybrid nanoparticles (Garg, Sharma, Singh, Nirbhavane, & Katare, 2015).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of stability indicating reversed‐phase liquid chromatographic method for simultaneous quantification of methotrexate and teriflunomide in nanoparticles and marketed formulation

Pandey

Mahtab

Singh

et al. 2018

Biomedical Chromatography

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Methotrexate (MTX) and teriflunomide (TEF) are the two most effective disease-modifying antirheumatic drugs used as combination therapy for rheumatoid arthritis and no robust high-performance liquid chromatography (HPLC) method is available for their simultaneous estimation to date. Therefore, we have developed and validated an isocratic reversed-phase HPLC method for simultaneous analysis of MTX and TEF spiked in the form of active pharmaceutical ingredients, tablets and nanoformulations. The best separation was achieved on a BDS, C , 4.6 × 250 mm, 5 μm analytical column (Thermo Hypersil) with methanol-ethylammonium formate-potassium dihydrogen phosphate buffer (55 mm, pH 3.5; 65:5:30, v/v) as mobile phase at a flow rate of 0.8 mL/min. All the samples were subjected to force degradation studies. Responses of MTX and TEF were found to be a linear function of concentration over the range 1-50 μg/mL (r = 0.9976 and 0.9982). The limits of detection and limit of quantification were 7.74 and 25.82 ng/mL and 10.74 and 35.80 ng/mL, respectively. Degradation products produced under the stress studies did not interfere with the detection of MTX and TEF and therefore the developed method can be regarded as stability indicating.

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HPLC-DAD and UV-Vis Spectrophotometric Methods for Methotrexate Assay in Different Biodegradable Microparticles

Cited by 12 publications

References 0 publications

Exploiting gelatin nanocarriers in the pulmonary delivery of methotrexate for lung cancer therapy

Exploiting gelatin nanocarriers in the pulmonary delivery of methotrexate for lung cancer therapy

Analytical methodologies for determination of methotrexate and its metabolites in pharmaceutical, biological and environmental samples

Development and validation of stability indicating reversed‐phase liquid chromatographic method for simultaneous quantification of methotrexate and teriflunomide in nanoparticles and marketed formulation

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