Classification and Identification of Petroleum Microorganisms by MALDI-TOF Mass Spectrometry

Angolini, Célio F. F.; Pilau, Eduardo Jorge; Lopes-Oliveira, Patrícia F.; García, Isabel Natalia Sierra; Gozzo, Fábio C.; Oliveira, Valéria Maia de; Marsaioli, Anita Jocelyne

doi:10.5935/0103-5053.20150004

Cited by 2 publications

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“…isolates derived from marine sponges while application of MALDI-TOF MS technique enabled discrimination of very closely related species with high confidence. Similar findings revealed Angolini et al [46] investigating petroleum microorganisms. It suggests that use of proteomic-based approaches such as MALDI-TOF MS is a good solution for distinguishing some species sharing very similar 16S rDNA sequences as in the case of honey bacteria, without the need to analyze additional genes.…”

Section: Discussionsupporting

confidence: 91%

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

et al. 2019

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In the presented work identification of microorganisms isolated from various types of honeys was performed. Martix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rDNA sequencing were applied to study environmental bacteria strains.With both approches, problematic spore-forming Bacillus spp , but also Staphylococcus spp ., Lysinibacillus spp ., Micrococcus spp . and Brevibacillus spp were identified. However, application of spectrometric technique allows for an unambiguous distinction between species/species groups e.g. B . subtilis or B . cereus groups. MALDI TOF MS and 16S rDNA sequencing allow for construction of phyloproteomic and phylogenetic trees of identified bacterial species. Furthermore, the correlation beetween physicochemical properties, geographical and botanical origin and the presence bacterial species in honey samples were investigated.

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Section: Discussionsupporting

confidence: 91%

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

et al. 2019

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“…MADLI-TOF MS has become a well-established and rapid method for characterizing and identifying bacteria based on protein profiles [61,62]. However, the restricted database of MADI-TOF MS leads to difficulties in identifying bacteria from various environmental samples [63,64]. Preference of Rhizobium sp.…”

Section: Discussionmentioning

confidence: 99%

Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota

et al. 2020

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Plant microbiota support the diversity and productivity of plants. Thus, cultivation-dependent approaches are indispensable for in vitro manipulation of hub taxa. Despite recent advances in high-throughput methods, cultivability is lagging behind other environmental microbiomes, notably the human microbiome. As a plant-based culturing strategy, we developed culture media based on a broth of cooked aqueous mixtures of host plants. This improved the in vitro growth of representative isolates of plant microbiota and extended the in situ recovery of plant microbiota. With clover, 16S rRNA gene sequencing of representative isolates confirmed the predominance of Firmicutes, Alphaproteobacteria and Gammaproteobacteria, and less frequently Bacteroidetes and Actinobacteria. Whereas bovine-based culture media (modified R2A) confined the diversity to Firmicutes, the plant broth-based culture media revealed a wider scope of endophytes beyond rhizobia, i.e., multiple genera such as Chryseobacterium, Cronobacter, Kosakonia, Tsukamurella, and a potentially/presumptive novel species. Matrix-assisted laser desorption/ionization time-of-flight (MADI-TOF) analysis clustered isolates according to their plant niches, the endo-phyllosphere/endo-rhizosphere. We recommend the plant broth for simplicity, reproducibility and perdurable storage, supporting future culturomics applications, good laboratory practice (GLP) and good manufacturing practice (GMP). The strategy creates an “in-situ-similis” vegan nutritional matrix to analyze microbial diversity and reveal novel microbial resources pertinent to biotechnological and environmental applications.

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Classification and Identification of Petroleum Microorganisms by MALDI-TOF Mass Spectrometry

Cited by 2 publications

References 1 publication

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

Analysis of bacteria associated with honeys of different geographical and botanical origin using two different identification approaches: MALDI-TOF MS and 16S rDNA PCR technique

Plant Broth- (Not Bovine-) Based Culture Media Provide the Most Compatible Vegan Nutrition for In Vitro Culturing and In Situ Probing of Plant Microbiota

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