Characterization and Quantification by Gas Chromatography of Free Steroids in Unsaponifiable Matter of Vegetable Oils

Bezerra, Kelly da S.; Filho, Nelson Roberto Antoniosi

doi:10.5935/0103-5053.20130288

Cited by 12 publications

(20 citation statements)

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“…The highest of β‐sitosterol concentration for ethanol as solvent were obtained under the test 8 (2 min and 80% of SV) (Table ). Literature shows that the concentration of β‐sitosterol in the oil of babassu almonds ranges from 48.2 to 53.9 mg/kg (Codex, ) and 380 mg/kg (Bezerra & Antoniosi Filho, ). The values obtained in our experiment are higher than the results of (Codex, ) and in the same order of magnitude of (Bezerra & Antoniosi Filho, ).…”

Section: Resultsmentioning

confidence: 99%

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Babassu almonds oil extracted with alternative pressurized green solvents, its triacylglycerol prediction and β‐sitosterol composition

Oliveira

Garcia

Mazalli³

et al. 2019

J Food Process Engineering

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The behavior of two process variables in the oil extraction from babassu almonds (percentage of solvent volume [SV], and the contact time between solvent and the matrix in each batch [St]) was evaluated. In the statistical evaluation, SV was a significant variable in the process that used ethanol as solvent. Conversely, when the solvent was isopropanol, the significant variable was St. The response surface analysis (RSA) indicated that high St, regardless of the solvent amount, results in high yields. The highest yields were 55.34% when isopropanol was the solvent and 53.12% for ethanol. These values represent 92.4 and 88.7% of the oil content in the seeds, respectively. The fatty acid profile, rich in lauric, myristic, oleic, and palmitic acids, was the same regardless of the solvent and operational conditions used. The extract obtained using pressurized isopropanol presented the highest concentration of β-sitosterol when 3 min was used as St with 66% of SV. When pressurized ethanol was the solvent, the highest concentration of β-sitosterol was obtained at 2 min of St and 80% of SV. As a function of fatty acid composition, the main triacylglycerols present in babassu oil were COL, LOL, LOM, LOP, and MOP regardless of solvent. Practical applicationsBabassu almond is rich in potassium, phosphorous, magnesium, calcium, and zinc, which are elemental minerals for human health. Being considered oleaginous, it is equally of great importance for cosmetic, pharmaceutical, and food industries.Babassu oil is rich in phytosterols (among them β-sitosterol) and it is rich in essential fatty acids such as lauric, myristic, oleic, and palmitic acids, which have clinical effects in the treatment of skin ulcers and burns.

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Section: Resultsmentioning

confidence: 99%

“…Babassu oil is rich in phytosterols, among them β‐sitosterol. The concentration of β‐sitosterol in babassu oil, according, to the Codex Alimentarium Commission (), is approximately 48.2–53.9 mg/kg and 380 mg/kg according to (Bezerra & Antoniosi Filho, ).…”

Section: Introductionmentioning

confidence: 99%

Babassu almonds oil extracted with alternative pressurized green solvents, its triacylglycerol prediction and β‐sitosterol composition

Oliveira

Garcia

Mazalli³

et al. 2019

J Food Process Engineering

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“…Although the occurrence of hydrocarbons in edible oils is well known (Cert, Moreda, & Pérez-Camino, 2000), there are not many detailed studies on the hydrocarbons present in each kind of vegetable oil. However, there are exceptions, such as the determination of polycyclic aromatic hydrocarbons of well known toxicity (Guillén & Sopelana, 2004), or of steroidal hydrocarbons (Bezerra & Antoniosi Filho, 2014;Cert et al, 2000) for fraud detection. Their determination usually requires their isolation from the unsaponifiable oil fraction and posterior separation by chromatographic techniques.…”

Section: Hydrocarbonsmentioning

confidence: 98%

“…Their presence in oils is associated with the refining process and an official method for the determination of one of them, 3,5-stigmastadiene, was established in order to detect frauds (Dobarganes, Cert, & Dieffenbacher, 1999). The development of methods for the determination of this latter hydrocarbon has received great attention in recent years (Bezerra & Antoniosi Filho, 2014) due to the interest in finding methods with advantages over the official method. With the methodology here used nine steroidal hydrocarbons have been found and their relative abundances determined.…”

Section: Hydrocarbonsmentioning

confidence: 99%

A new methodology capable of characterizing most volatile and less volatile minor edible oils components in a single chromatographic run without solvents or reagents. Detection of new components

et al. 2017

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“…GC–MS analysis offers influence measurements of reproducibility, dynamic range, and universal mass spectral library for compounds with small molecular weight 6 , 7 . On the other hand, LC–MS covers a large array of compounds predominant as secondary metabolites such as terpenoids and phenolics 8 .…”

Section: Introductionmentioning

confidence: 99%

LC–MS/MS and GC–MS profiling as well as the antimicrobial effect of leaves of selected Yucca species introduced to Egypt

Sayed

Basam

El-Naggar

et al. 2020

Sci Rep

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Few studies thoroughly investigated different Yucca species introduced to Egypt. As a part of our ongoing investigation of the Yucca species; Yucca aloifolia and its variety Yucca aloifolia variegata, Yucca filamentosa, and Yucca elephantipes (Asparagaceae) were extensively subjected to phytochemical and antimicrobial investigation. Yucca species cultivated in Egypt showed no antimicrobial effect. GC/MS of the lipoid contents of Y. aloifolia variegata was carried out. Twenty-six fatty acids were identified. Saturated fatty acids established almost twice the unsaturated ones and constituted 64.64% of which palmitic acid and palmitoleic acid signifying 58.28% and 30.98%, respectively. Hydrocarbons were 21 constituting 39.64% of the unsaponifiable fraction. Only three sterols 42.36% were detected, major was γ-sitosterol. LC–MS/MS comparison of the 4 plant extracts imply that Y.aloifolia variegata L extract was the richest, which was apparent through its superior biological activity. LC–MS/MS analysis of the total alcoholic extract (Alc) of the leaves of Y.aloifolia variegata L. was performed using MS-techniques at different voltages; equal to 35 and 135 eV. Negative and positive-ion modes analyses at low fragmentation energy allowed the tentative identification of 41 and 34 compounds, respectively. The LC–ESI–MS/MS analysis in the positive mode proved to be better in the identification of saponins.

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Characterization and Quantification by Gas Chromatography of Free Steroids in Unsaponifiable Matter of Vegetable Oils

Cited by 12 publications

References 0 publications

Babassu almonds oil extracted with alternative pressurized green solvents, its triacylglycerol prediction and β‐sitosterol composition

Babassu almonds oil extracted with alternative pressurized green solvents, its triacylglycerol prediction and β‐sitosterol composition

A new methodology capable of characterizing most volatile and less volatile minor edible oils components in a single chromatographic run without solvents or reagents. Detection of new components

LC–MS/MS and GC–MS profiling as well as the antimicrobial effect of leaves of selected Yucca species introduced to Egypt

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