Mesangial cells: renal function protagonists or coadjuvants?

Younes-Ibrahim, Maurício

doi:10.5935/0101-2800.20130041

Cited by 4 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mesangial cell that help and modulate the GFR and the surface area of the glomerulus, thanks to their ability to contract and relax in response to vasoactive drugs, may suffer significant proliferation, with glomerular sclerosis and tubulo-interstitial fibrosis in animals in response to the glomerular endothelial lesions caused by smoking. (21,22) Due to the morphological changes observed, as well as a lower water consumption by the SG animals, also observed in the studies by Gonçalves-Silva et al, (23) the biochemical parameters of blood and urine analysis also changed in the SG, providing subsidies to understand the possible damage to kidney function, since the levels of creatinine, urinary creatinine, and urinary volume, used to determine the creatinine clearance rate to estimate the GFR, were significantly lower. Smoking promotes a significant increase in the albumin-creatinine ratio, with changes in glomerular capillary pressure and kidney function, (24) and there are few studies correlating nicotine present in the bloodstream of passive smokers with kidney diseases, especially regarding acute exposure.…”

Section: ❚ Discussionmentioning

confidence: 63%

Morphofunctional study on the effects of passive smoking in kidneys of rats

Moraes¹,

Breda-Stella²,

Carvalho³

2021

Einstein (São Paulo)

View full text Add to dashboard Cite

Objective To analyze whether passive inhalation of cigarette smoke causes morphological, structural, and functional changes in kidneys of rats. Methods Wistar rats, aged eight weeks, weighing on average 260g, were divided into Control Group and Smoking Group. Each group was subdivided into four groups of ten animals for morphofunctional analysis, in a period of seven and 28 days. The Smoking Group was exposed to smoke of 40 cigarettes per day, at certain times and in automated equipment for cigarette burning, called smoking machine (SM-MC-01). After the exposure period, urine and blood samples were collected for the functional analyses, and the kidneys were dissected and submitted to histological procedures for morphoquantitative analyses. Results After exposure of animals of the Smoking Group, the following were observed: lower weight gain; lower water and feed intake; decreased renal weight, diameter, and volume; reduction in cortical thickness and glomerular volume density; decrease in glomerular and capsular diameter; increase in mesangial density; decreased urine volume; increased levels of glucose, serum creatinine and microalbuminuria; decreased urinary creatinine levels and creatinine clearance rate. Conclusion Passive smoking negatively influences renal morphology and glomerular filtration rate, with effects similar to those described in the literature regarding active smoking.

show abstract

Section: ❚ Discussionmentioning

confidence: 63%

Morphofunctional study on the effects of passive smoking in kidneys of rats

Moraes¹,

Breda-Stella²,

Carvalho³

2021

Einstein (São Paulo)

View full text Add to dashboard Cite

show abstract

“…LPS is commonly used to induce septic shock and subsequent AKI in rodent models [29][30][31]. Mesangial cell occupies a central position in renal glomerulus and is involved in the pathogenesis of many kidney diseases [32,33]. Therefore, in the present study, to explore the role of ALA in septic AKI in vitro, LPS was utilized to induce inflammatory responses in rat mesangial HBZY-1 cells.…”

Section: Discussionmentioning

confidence: 97%

Alpha-Lipoic Acid Exerts Anti-Inflammatory Effects on Lipopolysaccharide-Stimulated Rat Mesangial Cells via Inhibition of Nuclear Factor Kappa B (NF-κB) Signaling Pathway

Jia-hong

Zhao

et al. 2014

Inflammation

View full text Add to dashboard Cite

Sepsis is often initiated by invasive infection, characterized by overwhelming induction of pro-inflammatory cytokines. The incidence and mortality of sepsis and the associated development of acute kidney injury (AKI) remain high, and lines of research into potential treatments are needed. This study was conducted to investigate effects of alpha-lipoic acid (ALA) on septic AKI in vitro. ALA of 200 or 400 μM was used to pretreat rat HBZY-1 mesangial cells before commencement of 1 μg/mL lipopolysaccharide (LPS). Our data indicated that ALA pretreatment reduced LPS-stimulated release of inflammatory cytokines, such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-1 beta (IL-1β), as well as IL-6, in HBZY-1 cell supernatant. Moreover, LPS-induced expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) was inhibited by ALA pretreatment, and consequently, the secretion levels of their respective enzymatic products prostaglandin E2 (PGE2) and nitric oxide (NO) were significantly decreased. LPS-enhanced phosphorylation of nuclear factor kappa B (NF-κB) inhibitor alpha (IκBα) and IκB kinase alpha/beta (IKKα/β) and nuclear translocation of NF-κB subunit p65 in HBZY-1 cells were inhibited by ALA pretreatment. Additionally, the NF-κB inhibitor N-acetylcysteine (NAC) exerted similar inhibitory effects as ALA on COX-2 and iNOS expression. In summary, our study demonstrates that ALA mitigates LPS-induced inflammatory responses in rat mesangial cells probably via inhibition of NF-κB signaling pathway, suggesting a therapeutic potential of ALA in AKI related to sepsis.

show abstract

“…Therefore, this study was conducted to evaluate the potential effects of gold clusters on septic AKI in vitro. In the glomerulus, mesangial cells occupy a central position and participate in the pathogenesis of AKI [28]. LPS is usually used to induce septic AKI in animal models [29,30].…”

Section: Discussionmentioning

confidence: 99%

Gold Clusters Attenuate Inflammation in Rat Mesangial Cells via Inhibiting the Activation of NF-κB Pathway

et al. 2020

View full text Add to dashboard Cite

Sepsis-induced acute kidney injury (AKI) with high incidence and mortality rates remains a great challenge in the clinic; thus, novel therapies need to be developed urgently. This complication is associated with an overwhelming systemic inflammatory response. The aim of this study was to evaluate the potential effects and possible mechanisms of gold clusters on septic AKI in vitro. Rat mesangial HBZY-1 cells were treated with peptide-templated gold clusters under lipopolysaccharide (LPS) stimulation. The LPS-induced expression of pro-inflammatory cytokines was measured, including tumor necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). Our data showed that the LPS-induced transcription and secretion of these cytokines were suppressed by pretreatment of gold clusters in a dose-dependent manner. Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) also play key roles in septic AKI and both of them are induced upon LPS-stimulation in mesangial cells. Our results further showed that pretreatment with gold clusters dramatically inhibited the LPS-stimulated transcription and expression of COX2 and iNOS, and the subsequent prostaglandin E2 (PGE2) and nitric oxide (NO) production in HBZY-1 cells. Since these factors are involved in the NF-κB pathway upon LPS stimulation, the potential roles of gold clusters on the NF-κB pathway were further determined. We found that LPS-induced NF-κB activation was suppressed in gold clusters-pretreated HBZY-1 cells. These results demonstrated that gold clusters can attenuate LPS-induced inflammation in mesangial cells, probably via inhibiting the activation of the NF-κB pathway, suggesting a potential therapeutic approach for septic AKI.

show abstract

Mesangial cells: renal function protagonists or coadjuvants?

Cited by 4 publications

References 6 publications

Morphofunctional study on the effects of passive smoking in kidneys of rats

Morphofunctional study on the effects of passive smoking in kidneys of rats

Alpha-Lipoic Acid Exerts Anti-Inflammatory Effects on Lipopolysaccharide-Stimulated Rat Mesangial Cells via Inhibition of Nuclear Factor Kappa B (NF-κB) Signaling Pathway

Gold Clusters Attenuate Inflammation in Rat Mesangial Cells via Inhibiting the Activation of NF-κB Pathway

Contact Info

Product

Resources

About