An Electrochemical Biosensor Based on the Tyrosinase Enzyme for the Determination of Phenol in Wastewater

Oliveira, Dejane P. C. de; Ribeiro, Francisco W.P.; Becker, Helena; Lima‐Neto, Pedro de; Correia, Adriana N.

doi:10.5935/0100-4042.20150086

Cited by 5 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, efforts have been made to develop faster electroanalytical techniques. De Oliveira et al fabricated an efficient biosensor based on tyrosinase, gold nanoparticles, MWCNTs and cobalt(II) phthalocyanine for determination of phenol in wastewater [79]. Tetrasulfonic acid substituted zinc(II) phthalocyanine was adsorbed on oxidized exfoliated graphite powder by an electrostatic self-assembly method and deposited on GCE.…”

Section: Environmental Pollutant Sensingmentioning

confidence: 99%

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

2021

View full text Add to dashboard Cite

Phthalocyanines and porphyrazines as macrocyclic aza-analogues of well-known porphyrins were deposited on diverse carbon-based nanomaterials and investigated as sensing devices. The extended π-conjugated electron system of these macrocycles influences their ability to create stable hybrid systems with graphene or carbon nanotubes commonly based on π–π stacking interactions. During a 15-year period, the electrodes modified by deposition of these systems have been applied for the determination of diverse analytes, such as food pollutants, heavy metals, catecholamines, thiols, glucose, peroxides, some active pharmaceutical ingredients, and poisonous gases. These procedures have also taken place, on occasion, in the presence of various polymers, ionic liquids, and other moieties. In the review, studies are presented that were performed for sensing purposes, involving azaporphyrins embedded on graphene, graphene oxide or carbon nanotubes (both single and multi-walled ones). Moreover, possible methods of electrode fabrication, limits of detection of each analyte, as well as examples of macrocyclic compounds applied as sensing materials, are critically discussed.

show abstract

Section: Environmental Pollutant Sensingmentioning

confidence: 99%

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

2021

View full text Add to dashboard Cite

show abstract

“…However, they require multi-stage sampling, which results in longer analysis times. On the other hand, biosensors based on microorganisms [ 18 ], and immobilized enzymes [ 19 , 20 ] overcome this limitation. They enable quick analysis and reusable biosensors, but they either have insufficient sensitivity or are difficult to manufacture and maintain.…”

Section: Introductionmentioning

confidence: 99%

Determination of Phenol with Peroxidase Immobilized on CaCO3

Kim

Дубровский

Musin

et al. 2023

IJMS

View full text Add to dashboard Cite

Phenols are widely used in industries despite their toxicity, which requires governments to limit their concentration in water to 5 mg/L before discharge to the city sewer. Thus, it is essential to develop a rapid, simple, and low-cost detection method for phenol. This study explored two pathways of peroxidase immobilization to develop a phenol detection system: peroxidase encapsulation into polyelectrolyte microcapsules and peroxidase captured by CaCO3. The encapsulation of peroxidase decreased enzyme activity by 96%; thus, this method cannot be used for detection systems. The capturing process of peroxidase by CaCO3 microspherulites did not affect the maximum reaction rate and the Michaelis constant of peroxidase. The native peroxidase—Vmax = 109 µM/min, Km = 994 µM; CaCO3–peroxidase—Vmax = 93.5 µM/min, Km = 956 µM. Ultimately, a reusable phenol detection system based on CaCO3 microparticles with immobilized peroxidase was developed, capable of detecting phenol in the range of 700 ng/mL to 14 µg/mL, with an error not exceeding 5%, and having a relatively low cost and production time. The efficiency of the system was confirmed by determining the content of phenol in a paintwork product.

show abstract

“…Os biossensores são dispositivos nos quais uma enzima ou uma proteína são utilizadas para medir de modo seletivo determinadas substâncias em um meio específico (OLIVEIRA, RIBEIRO, et al, 2015), ou seja, são pequenos dispositivos que utilizam reações biológicas para detecção de analitos alvo, combinando um componente biológico que interage com um substrato-alvo a um transdutor físico, convertendo os processos de reconhecimento em sinais mensuráveis (CALIL, et al, 2015). A utilização desse tipo de sensor traz muitas vantagens, entre elas a alta sensibilidade e seletividade destes, como também a facilidade em termos de desenvolvimento do sensor, sendo assim acessíveis e prontos para o uso.…”

Section: Introductionunclassified

Investigação Do Sinal Amperométrico Na Parede Externa De Um Biossensor Do Tipo Disco De Biofilme Poroso

Santos¹,

Cardoso²,

Gomes³

et al. 2019

Blucher Chemical Engineering Proceedings

View full text Add to dashboard Cite

An Electrochemical Biosensor Based on the Tyrosinase Enzyme for the Determination of Phenol in Wastewater

Cited by 5 publications

References 2 publications

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

Azaporphyrins Embedded on Carbon-Based Nanomaterials for Potential Use in Electrochemical Sensing—A Review

Determination of Phenol with Peroxidase Immobilized on CaCO3

Investigação Do Sinal Amperométrico Na Parede Externa De Um Biossensor Do Tipo Disco De Biofilme Poroso

Contact Info

Product

Resources

About