Microbial contamination of cuffs lab coats during health care

Abstract

RESUMOO estudo objetivou avaliar a contaminação bacteriana em jalecos de acadêmicos de enfermagem após a sua utilização em práticas assistenciais à saúde. Realizou-se estudo quantitativo e descritivo, no qual os jalecos dos acadêmicos foram recolhidos, lavados e passados de forma padronizada e usados durante quatro horas em atividades assistenciais. Subsequentemente, coletaram-se amostras dos punhos com hastes de algodão estéreis para serem incubadas; analisando-se posteriormente o crescimento microbiano por meio de análise morfológica pelo método Gram e antibiograma. Observou-se crescimento bacteriano dos microrganismos Staphylococcus aureus e Staphylococcus epidermidis em 50% das amostras coletadas, sendo que o Staphylococcus aureus, encontrado no ambulatório de atendimento a portadores de feridas, apresentou resistência aos antibióticos Vancomicina, Cloranfenicol e Sulfonamidas. Os dados demonstraram que os jalecos utilizados durante as atividades assistenciais, mesmo em curtos períodos, são contaminados por cepas resistentes a antibióticos e que potencialmente podem ser causadores de infecção relacionada a assistência à saúde. Descritores: Staphylococcus Aureus; Enfermagem; Infecção Hospitalar. ABSTRACTThe study aimed to evaluate the bacterial contamination in lab coats worn by nursing students, before and after being worn in health care practices. A quantitative and descriptive study was carried out, in which the students' coats were collected, washed and ironed in a standardized way and wore for four hours in assistance activities. Subsequently, samples from the cuffs were collected with sterile cotton swabs in order to be incubated in order to analyze microbial growth through morphological analysis by Gram's Method and antibiogram. There was bacterial growth of Staphylococcus aureus and Staphylococcus epidermidis in 50% of the collected samples and Staphylococcus aureus, found in patients with wounds in outpatient care, showed resistance to antibiotics such as Vancomycin, Chloramphenicol and Sulfonamides. The data demonstrated that the lab coats wore during assistance activities, even in short periods, are effectively contaminated by strains resistant to antibiotics and can potentially cause infection related to health care. Key words: Staphylococcus Aureus; Nursing; Cross Infection. RESUMENObjetivó-se evaluar la contaminación bacteriana de batas de laboratorio de estudiantes de enfermería, antes y después del uso en la asistencia a la salud. Realizó-se un estudio cuantitativo y descriptivo, en el que fueron recogidas las batas de los alumnos, lavadas y planchadas de manera estandarizada y usadas durante cuatro horas en actividades de asistencia. Seguidamente, se recolectaron muestras de los puños con hisopos de algodón estéril para ser incubadas; analizando-se posteriormente el crecimiento microbiano mediante análisis morfológico por el método de Gram y el antibiograma. Hubo crecimiento bacteriano de microorganismos Staphylococcus aureus y Staphylococcus epidermidis en 50% de las muestras recolectadas, s...

Determination of antimicrobial susceptibility and biofilm production in Staphylococcus aureus isolated from white coats of health university students

“…The rate of S. aureus isolates found herein is higher than in other studies. Fenalte et al [19] have detected this microorganism in 19.8% of samples from coats worn by nursing professionals in a midsize hospital, while Margarido et al [20] identified S. aureus in 31.6% of samples from nursing students. The data presented in this study highlight S. aureus colonization in coats worn by students less exposed to hospital environment than those in other health science courses.…”

“…Students should therefore receive proper education regarding biosafety procedures, especially after classes and lab research activities. Ideally, white coats should be disposable, but since this practical is not viable it is mandatory that their use follow rigorous sanitization measures, including separate laundering with powder detergent followed by drying and ironing, which can prevent the possibility of bacterial pathogen dissemination, according to Margarido et al [20]. These authors collected samples from white coats sanitized according to standard procedures, i.e.…”

Fungal contamination in white medical coats of dentistry professionals

“…Several reports of contamination in lab coats with different bacterial species such as Staphylococcus, Streptococcus and Enterococcus can be found in the literature [5]. However, there are few reports of fungal contamination in lab coats, which does not mean that fungal contamination is uncommon or less harmful than the bacterial contamination in this type of PPE.…”

Práctica de inyecciones seguras por parte del personal de la salud: revisión integrativa

“…Las consecuencias de las PInS son consideradas eventos adversos reales a gran escala (24)(25)(26)(27)(28)(29), problemática resaltada por la Organización Mundial de la Salud como una prioridad de investigación (4). El objetivo de la revisión fue analizar la evidencia disponible sobre las PIS ejecutadas por el personal de la salud en su práctica clínica.…”