Dynamic thiol/disulfide homeostasis in patients with age-related macular
            degeneration

Aktaş, Serdar; Sağdık, Hacı Murat; Tetikoğlu, Mehm et; Aktaş, Hatice; Özçura, Fatih; Uçar, Fatma; Alışık, Murat; Ergin, Merve

doi:10.5935/0004-2749.20170057

Cited by 6 publications

(3 citation statements)

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“…Other previous research indicated that decreased glutathione and cysteinylglycine levels with the concomitant increase of oxidized thiols were correlated with age [53]. Other reports have indicated that serum free thiols may play an important role in protecting tissue against oxidative stress and the thiol/disulfide balance is a key protective factor, rather than the total thiol concentration [54][55][56]. Nevertheless, we did not evaluate the serum free thiols in this study, and this requires further clarification.…”

Section: Discussionmentioning

confidence: 71%

Evaluation of Redox Profiles of the Serum and Aqueous Humor in Patients with Primary Open-Angle Glaucoma and Exfoliation Glaucoma

et al. 2020

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Oxidative stress is thought to play a significant role in the development of glaucoma. However, the association between systemic and local oxidative stresses in different types of glaucoma has not been assessed fully. The current study compared the redox status in the aqueous humor (AH) and blood samples among eyes with primary open-angle glaucoma (POAG), exfoliation glaucoma (EXG), and non-glaucomatous controls to evaluate the relationship among systemic redox status, intraocular oxidative stress, and clinical backgrounds. AH and blood samples were obtained from 45 eyes of 45 Japanese subjects (15 POAG, 15 EXG, and 15 control eyes). The serum levels of lipid peroxides, ferric-reducing activity, and thiol antioxidant activity were measured by diacron reactive oxygen metabolites (dROM), biologic antioxidant potential (BAP), and sulfhydryl (SH) tests, respectively, using a free radical analyzer. The activities of cytosolic and mitochondrial forms of the superoxide dismutase (SOD) isoforms, i.e., SOD1 and SOD2, respectively, in AH and serum were measured using a multiplex bead immunoassay. In AH, SOD1 in subjects with EXG and SOD2 in those with POAG and EXG were significantly higher than in control eyes. In serum, compared to control subjects, BAP in subjects with POAG and EXG was significantly lower; SOD1 in those with EXG and SOD2 in those with POAG and EXG were significantly higher. dROM and SH did not differ significantly among the groups. The BAP values were correlated negatively with the SOD1 concentrations in AH and serum, SOD2 in the AH, intraocular pressure, and number of antiglaucoma medications. In conclusion, lower systemic antioxidant capacity accompanies up-regulation of higher local antioxidant enzymes, suggesting increased oxidative stress in eyes with OAG, especially in EXG. Determination of the systemic BAP values may help predict the redox status in AH.

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Section: Discussionmentioning

confidence: 71%

Evaluation of Redox Profiles of the Serum and Aqueous Humor in Patients with Primary Open-Angle Glaucoma and Exfoliation Glaucoma

et al. 2020

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“…In another study, authors have investigated age-related macular degeneration and they have established that NT levels were significantly decreased and disulfide levels were significantly increased in patients compared to control subjects [82].…”

Section: Ophthalmic Disordersmentioning

confidence: 99%

Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects

Erel

Erdoğan

2020

Turk J Med Sci

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Dynamic thiol-disulfide homeostasis (TDH) is a new area has begun to attract more scrutiny. Dynamic TDH is reversal of thiol oxidation in proteins and represents the status of thiols (-SH) and disulfides (-S-S-). Organic compounds containing the sulfhydryl group is called thiol, composed of sulfur and hydrogen atoms. Disulfides are the most important class of dynamic, redox responsive covalent bonds build in between two thiol groups. For many years, thiol levels were analyzed by several methods. During last years, measurements of disulfide levels have been analyzed by a novel automated method, developed by Erel and Neselioglu. In this method, addition to thiol-termed as native thiol-levels, disulfide levels were also measured and sum of native thiol and disulfide levels were termed as total thiol. Therefore, TDH was begun to be understood in organism. In healthy humans, TDH is maintained within a certain range. Dysregulated dynamic TDH has been implicated several disorders with unknown etiology. A growing body of evidence has demonstrated that the thiol-disulfide homeostasis is involved in variety diseases, such as diabetes mellitus, hypertension, non-small cell lung cancer, Familial Mediterranean fever, inflammatory bowel diseases, occupational diseases, gestational diabetes mellitus and preeclampsia. These results may elucidate some pathogenic mechanism or may be a predictor indicating diagnostic clue, prognostic marker or therapeutic sign. In conclusion, protection of the thiol disulfide homeostasis is of great importance for the human being. Evidence achieved so far has proposed that thiol-disulfide homeostasis is an important issue needs to elucidate wholly.

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“…The role of oxidative stress in pathogenesis of various ocular disease including the cataracts, age related macular degeneration, central serous chorioretinopathy, pterygium, dry eye and the keratoconus were reported before. 3,[12][13][14][15][16][17][18] Altınisik and et al showed increased oxidative stress index (OSI) and lower total antioxidant status (TAS) in the humor aqueous samples of the retinal vein occlusion patients and they blamed hypoxia, ischemia and reperfusion damage by the reactive oxygen radicals. 3 Becatti et al found higher reactive oxygen species production and membrane lipid peroxidation in the erythrocytes of the retinal vein occlusion patients due to the oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?

Polat¹,

Kurt²,

Kılıç³

et al. 2019

Turkiye Klinikleri J Ophthalmol

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In this study, it is aimed to investigate the dynamic thiol-disulfide homeostasis which is an indicator of oxidative stress in retinal vein occlusion patients. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Thirty nine retinal vein occlusion patients and 38 age-gender matched control subjects were included in the study. Exclusion criteria for retinal vein occlusion group were: ocular history and symptoms exceeding 3 weeks, any other history of ocular disease except the retinal vein occlusion, any previous ocular surgeries or lasers, any previous treatment or intra-vitreal injection for the retinal vein occlusion, any other systemic disease except the arterial hypertension. The control group included patients without any ocular or systemic diseases. Native thiol, total thiol, disulfide levels were studied in the serum samples of all participants and native/total thiol and disulfide/total thiol ratios were calculated as percentage. R Re es su ul lt ts s: : Total thiol, native thiol, disulfide, native/total thiol and disulfide/total thiol ratio were not significantly different between the retinal vein occlusion and the control groups. In the control group, age was inversely correlated with the total thiol level, but not with the other serum parameters. In the retinal vein occlusion group, there was no correlation among the age and the serum parameters but the body mass index (BMI) was inversely correlated with the total thiol level. C Co on nc cl lu us si io on n: : There was not any significant difference between the retinal vein occlusion patients and the control subjects for the thiol-disulfide homeostasis. K Ke ey yw wo or rd ds s: : Retinal vein occlusion; thiol-disulfide homeostasis; thiol; disulfide Ö ÖZ ZE ET T A Am ma aç ç: : Bu çalışmada retinal ven tıkanıklığı ile bir oksidatif stres belirteci olan dinamik tiyol-disülfit dengesi arasındaki ilişkinin araştırılması amaçlanmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Otuz dokuz retinal ven tıkanıklığı hastası ve 38 yaş ve cinsiyet eşleştirilmiş kontrol birey olmak üzere toplam 77 katılımcı çalışmaya alınmıştır. Retinal ven tıkanıklığı grubu için çalışmadan dışlanma kriterleri: 3 haftayı aşan göz şikayet ve bulguları, retinal ven tıkanıklığı dışında başka oküler hastalık öyküsünün olması, daha önce oküler cerrahi ve lazer öyküsünün olması, retinal ven tıkanıklığı için daha önce intravitreal enjeksiyon dahil olmak üzere herhangi bir tedavi uygulanmış olması, arteryel hipertansiyon dışında sistemik hastalık öyküsünün olması olarak belirlenmiştir. Kontrol grubu sistemik ve oküler hastalığı olmayan katılımcılardı. Native tiyol, total thiol, disülfit düzeyleri katılımcıların kan serumu örneklerinde çalışılarak belirlenmiştir ve native/total tiyol ve disülfit/total tiyol oranları hesaplanarak yüzde olarak kaydedilmiştir. B Bu ul lg gu u-l la ar r: : Total tiyol, native tiyol, disülfit, native/total tiyol oranı ve disülfit/total tiyol oranları retinal ven tıkanıklığı ve kontrol grubu arasında karşılaştırıldığında anlamlı fark bulunmamış...

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Dynamic thiol/disulfide homeostasis in patients with age-related macular degeneration

Abstract: The antioxidant/oxidant balance demonstrated by dynamic TDH is shifted to the oxidative side in patients with AMD.

Cited by 6 publications

References 0 publications

Evaluation of Redox Profiles of the Serum and Aqueous Humor in Patients with Primary Open-Angle Glaucoma and Exfoliation Glaucoma

Evaluation of Redox Profiles of the Serum and Aqueous Humor in Patients with Primary Open-Angle Glaucoma and Exfoliation Glaucoma

Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects

Is There Any Association Between the Retinal Vein Occlusion and the Thiol-Disulfifide Homeostasis Which is an Oxidative Stress Indicator?

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