Efeito do intervalo de imersão e de injeção de ar na multiplicação &lt;i&gt;in vitro&lt;/i&gt; de &lt;i&gt;Eucalyptus grandis x Eucalyptus urophylla&lt;/i&gt; em biorreator de imersão temporária

Oliveira, Mila Liparize de; Xavier, Aloísio; Filho, Ricardo Miguel Penchel; Reis, Jocemar Palauro dos

doi:10.5902/1980509813321

Cited by 5 publications

(5 citation statements)

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“…Again, the use of the nutrients of the MS medium was significantly favorable for the root development of 'Pircinque' strawberry roots, but more prominently when it was kept in contact with the explants five times per day. These data do not corroborate the results of Oliveira et al (2014), who concluded that in the majority of cases, the smaller intervals between the immersions favored the development of the cultures by the greater use of the liquid culture medium by the plant. Figure 3A shows explant and shoot growth (cm), where explant length was higher under immersion conditions of 5x/day in liquid MS culture medium.…”

Section: Resultscontrasting

confidence: 96%

“…It is verified that for both the average number of leaves and roots, the bioreactor system of temporary immersion of liquid culture medium favored the development of 'Pircinque' strawberry explants. According to Oliveira et al (2014), this result confirms that root proliferation depends on the availability of water and nutrients in the surrounding microenvironment. In this sense, if this microenvironment is low in nutrients or very dry, root growth is slow, and, as conditions improve, root growth tends to increase (TAIZ and ZEIGER, 2013).…”

Section: Resultssupporting

confidence: 57%

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Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

et al. 2019

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The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

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Section: Resultscontrasting

confidence: 96%

Section: Resultssupporting

confidence: 57%

Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

et al. 2019

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“…A higher number of shoots per explant was observed at higher sucrose concentrations, ranging from 18.3 to 23.9 (table 3). These results surpass those observed by Oliveira et al (2011), who obtained approximately 10 shoots per explant after 28 days of cultivating Eucalyptus grandis W. Hill ex Maiden. x E. urophylla S. T. Blake clones in semi-solid Murashige and Skoog medium supplemented with 30 g L -1 of sucrose, which is half of what we found (20.0) (table 3).…”

Section: Treatmentscontrasting

confidence: 58%

“…For example, the use of bioreactors containing liquid media facilitates the rapid uptake of nutrients through submersion in the nutrient-rich medium and may serve as an alternative for cultivating Eucalyptus spp. clones (Oliveira et al 2011).…”

Section: Treatmentsmentioning

confidence: 99%

Influence of sucrose and medium consistency on in vitro multiplication and photosynthetic pigment profile of Eucalyptus saligna

Reisdörfer-Schorr,

Ikeda,

de-Alcantara

2023

Bosque (Valdivia)

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In southern Brazil, Eucalyptus saligna is one of the most cultivated species within the Eucalyptus genus for forest plantations, thanks to genetic improvement programs. Among the primary vegetative propagation methods for recalcitrant genotypes is micropropagation. This study aimed to investigate the effects of two variables in the culture medium on the micropropagation of E. saligna. Nodal segments of 2 cm were introduced into a Murashige and Skoog medium supplemented with 0, 15, 30, and 45 g L -1 of sucrose, considering two different medium consistencies: semi-solid (7.5 g L -1 of agar), and liquid with a spherical solid support. The experiment followed a completely randomized design, with eight treatments in a factorial scheme and four replicates, each containing 10 explants. After 30 days, several parameters were measured, including the mortality rate, number of shoots per explant, average shoot length, chlorophyll a, b, and total chlorophyll content. The results indicated that the survival and multiplication rates of explants were higher in the semi-solid medium. In contrast, the liquid medium with solid support proved inefficient, as it failed to establish adequate contact between the explants and the culture medium, compromising the nutrient supply. Generally, survival rates were higher in treatments with increased sucrose concentrations, as higher sucrose levels provide metabolic energy and carbon skeletons essential for the biosynthesis of amino acids, proteins, polysaccharides, and other organic compounds required for growth. Furthermore, the higher sucrose concentration promoted shoot formation, although there was no statistically significant difference among the treatments supplemented with sucrose concentrations of 15, 30, and 45 g L -1 . The higher sucrose concentration resulted in a significantly reduced chlorophyll content in the semi-solid medium.

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“…Specific innovations have been proposed for the application of large-scale micropropagation technologies in Eucalyptus (Xavier et al, 2013), such as the manipulation of the in vitro atmosphere and/or environment (e.g., photoautotrophic propagation) (Kozai, 2010); the use of temporary immersion and liquid medium bioreactors (Oliveira et al, 2011(Oliveira et al, , 2014; the replacement of the semisolid medium with alternative substrates (Kirdmanee, et al, 1995); the automation and mechanization of systems operations (Penchel et al, 2007); and the use of in vitro microclonal hedge.…”

Section: Introductionmentioning

confidence: 99%

IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBRID CLONES.1

et al. 2017

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This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucalyptus grandis x E. urophylla clone and every 30 days for Eucalyptus urophylla x E. globulus clone. The following variables were evaluated under in vitro conditions: number of shoots > 0.5 cm, number of microcuttings > 2 cm, length of the longest microcutting, and shoots vigor. Under ex vitro conditions, in the greenhouse and shade house, the following variables were evaluated: seedling height, percentage of survival, stem diameter, percentage of root observed at the lower end of the tube, and seedling vigor. In full sun (ex vitro), the following variables were analyzed: seedling height, stem diameter, survival, number of roots, root volume, seedling vigor, and shoot and root dry matter. Good in vitro microcuttings productivity was observed over the successive collections. IBA levels were adjusted for each clone, ranging from 0.25 to 0.50 mg L-1 for Eucalyptus grandis x E. urophylla clone, and from 0.75 to 1.0 mg L-1 for Eucalyptus urophylla x E. globulus clone. IBA concentrations led to residual effects under ex vitro conditions, providing good rooting and survival for Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones at IBA concentrations between 0.25 and 0.50 mg L-1 and between 0.50 and 1.0 mg L-1, respectively.

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Efeito do intervalo de imersão e de injeção de ar na multiplicação <i>in vitro</i> de <i>Eucalyptus grandis x Eucalyptus urophylla</i> em biorreator de imersão temporária

Cited by 5 publications

References 0 publications

Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

Influence of sucrose and medium consistency on in vitro multiplication and photosynthetic pigment profile of Eucalyptus saligna

IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBRID CLONES.1

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