Molecular matching of red blood cells is superior to serological matching in sickle cell disease patients

Costa, Daiane Cobianchi da; Pellegrino, J.; Guelsin, Gláucia Andréia Soares; Ribeiro, Karina Antero Rosa; Gilli, Simone Cristina Olenscki; Castilho, Lilian

doi:10.5581/1516-8484.20130012

Cited by 24 publications

(25 citation statements)

References 21 publications

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“…Furthermore, there are well-established blood group genotyping protocols validated for the Brazilian population (e.g. Paraná State in Southern Brazil) 14, 15, 16, 17. However, due to the ethnic diversity in the country, blood group polymorphisms should be investigated in the different regions of Brazil.…”

Section: Introductionmentioning

confidence: 99%

Frequencies of polymorphisms of the Rh, Kell, Kidd, Duffy and Diego systems of Santa Catarina, Southern Brazil

Costa

Schinaider

Santos

et al. 2016

Revista Brasileira de Hematologia e Hemoterapia

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BackgroundRed blood cell genes are highly polymorphic with the distribution of alleles varying between different populations and ethnic groups. The objective of this study was to investigate gene polymorphisms of blood groups in the state of Santa Catarina, Southern Brazil.MethodsThree hundred and seventy-three unrelated blood donors and 31 transfusion-dependent patients were evaluated to investigate polymorphisms of the Rh, Kell, Duffy, Kidd, and Diego blood group systems in a population from the state of Santa Catarina. The subjects, from seven regions that comprise the blood-banking network of the state, were assessed between August 2011 and March 2014. The genotypes of the Rh, Kell, Duffy, Kidd, and Diego systems were determined using the restriction fragment length polymorphism-polymerase chain reaction and allele-specific polymerase chain reaction techniques.ResultsThe genotype frequencies in this study were significantly different when populations from different regions of Santa Catarina were compared. Furthermore, there were also significant differences in the genetic frequencies compared to other Brazilian states. The genotype frequencies of the Kell and Kidd blood groups are similar to European populations from Naples, Italy and Zurich, Switzerland.ConclusionThis article reports for the first time the frequency of polymorphisms of blood group systems in blood donors from Santa Catarina, Southern Brazil.

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Section: Introductionmentioning

confidence: 99%

Frequencies of polymorphisms of the Rh, Kell, Kidd, Duffy and Diego systems of Santa Catarina, Southern Brazil

Costa

Schinaider

Santos

et al. 2016

Revista Brasileira de Hematologia e Hemoterapia

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“…In a study performed in 2009 [10], we observed that in alloimmunized patients, who had been in need for transfusions each week, the transfusion interval could be increased to 30-45 days when 2-3 units of genotype-matched RBCs were given. After that, we also provided evidence that molecular matching of RBCs is superior to serological matching in SCD patients by showing that patients who previously had been received serologically matching units benefited from being transfused with molecularly matched units in terms of better in vivo RBC survival [11]. Therefore, according to our experience, matching at DNA level can provide an added level of safety and efficacy for chronically transfused patients with SCD and can be used to decrease the risk of alloimmunization.…”

Section: Antigen-matched In Sickle Cell Disease Patients: Chancesmentioning

confidence: 93%

Optimized Antigen-Matched in Sickle Cell Disease Patients: Chances and Challenges in Molecular Times - the Brazilian Way

Castilho

Dinardo

2018

Transfus Med Hemother

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The development of red blood cell (RBC) alloantibodies and autoantibodies complicates transfusion therapy in sickle cell disease (SCD) patients. In an effort to reduce the risk of alloimmunization, some strategies have been used to provide antigen-matched RBC transfusions to patients with SCD in Brazil, including molecular matching in 3 levels: RH and K matching; extended matching (RH, KEL, FY, JK, MNS, DI), and extended matching including RHD and RHCE variant alleles. Molecular matching has shown clinical benefits to the patients with SCD, contributing significantly to reduce the rates of alloimmunization. Improvements in the clinical outcomes of the patients have also been observed as shown by an increase in their hemoglobin levels and reduction in their percentage of hemoglobin S as well as better in vivo RBC survival and diminished frequency of transfusions. However, prevention of RBC alloimmunization still remains a challenge in Brazil due to the difficulty to fulfill all transfusion requests of the patients with antigen-matching units, inaccuracy of RBC phenotyping, RBC transfusions outside the institution where the patient is treated, advanced age of some patients, the RBC antigen discrepancy between donors and recipients, and the presence of RH variants.

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“…A fenotipagem é preconizada para os antígenos C, c, E, e, e K, mais imunogênicos, permitindo assim reduzir significativamente a produção de aloanticorpos antieritrocitários. Já a genotipagem é apontada como técnica para elucidação de casos onde a fenotipagem é ineficaz para conclusão dos resultados (COSTA, 2013;CHOU, 2013;WILKINSON, 2011;SLOAN, 2016).…”

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Aloimunização Eritrocitária Em Pacientes Com Anemia Falciforme Atendidos No Hemocentro De Caruaru, Pernambuco, Brasil

et al. 2018

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Objetivos: A aloimunização eritrocitária é um problema grave em pacientes que recebem transfusões, principalmente no tratamento das hemoglobinopatias, como a anemia falciforme. Os objetivos desse trabalho são estabelecer a frequência da aloimunização eritrocitária em pacientes com anemia falciforme e apontar os fatores envolvidos na aloimunização. Métodos: Estudo descritivo transversal retrospectivo de prevalência, sendo levantadas informações sobre aloimunização e fatores associados, em pacientes com anemia falciforme atendidos pelo Hemocentro de Caruaru, entre janeiro de 1987 a dezembro de 2012. As informações foram obtidas a partir da consulta a dados no sistema de prontuários médicos do Hemocentro, tabuladas em planilhas do programa Excel 2007 (Microsoft Office®) e analisadas estatisticamente através do software R, utilizando o Teste Exato de Fisher e OddsRatio. Resultados: A produção de anticorpos irregulares foi identificada em 11 pacientes, representando uma taxa de aloimunização de 34%. Os anticorpos encontrados foram: 05 anti-C (25%), 05 anti-E (25%), 03 anti-K (15%), 01 anti-D (5%), 01 anti-Cw (5%), 01 anti-Fya (5%), 01 anti-Jka (5%), 01 anti-S (5%), 01 anti-Lea (5%) e 01 anti-Leb (5%). Observou-se que indivíduos com idade acima de 30 anos e pacientes que receberam acima de 10 concentrados de hemácias tiveram maior risco em desenvolver aloanticorpos. Conclusão: Transfusões de hemácias continuam sendo essenciais para o tratamento de complicações da anemia falciforme. A realização da fenotipagem eritrocitária poderá contribuir para reduzir a alosensibilização e consequentemente o número de reações transfusionais hemolíticas, aumentando a segurança transfusional e influenciando na qualidade de vida destes pacientes.

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Molecular matching of red blood cells is superior to serological matching in sickle cell disease patients

Cited by 24 publications

References 21 publications

Frequencies of polymorphisms of the Rh, Kell, Kidd, Duffy and Diego systems of Santa Catarina, Southern Brazil

Frequencies of polymorphisms of the Rh, Kell, Kidd, Duffy and Diego systems of Santa Catarina, Southern Brazil

Optimized Antigen-Matched in Sickle Cell Disease Patients: Chances and Challenges in Molecular Times - the Brazilian Way

Aloimunização Eritrocitária Em Pacientes Com Anemia Falciforme Atendidos No Hemocentro De Caruaru, Pernambuco, Brasil

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