Comparison of cytokine levels and metabolic parameters of stored platelet concentrates of the Fundação Hemominas, Belo Horizonte, Brazil

Abstract

BackgroundProlonged storage of platelets could improve availability and logistical management and decrease wastage. Immunobiochemical methods can be used to guarantee the quality of platelets after prolonged storage.ObjectiveThe aim of this study was to compare storage-related changes in buffy coat-derived platelet concentrations versus platelet-rich plasmal.MethodsUnits of whole blood were drawn using a quadruple-bag blood container system. Platelet-rich plasma and buffy coat prepared from whole blood following standard methods were stored for 9 days. During this period test samples were aseptically collected for analysis on Days 1, 2, 3, 5, 7 and 9.ResultsThe highest CD42b expression was greater than 95%. The percentage of CD62p was significantly lower than the CD42b expression. The pH remained fairly stable during storage. Measurement of pO2 and pCO2 showed that oxygen levels were significantly higher than carbon dioxide levels. There were no significant differences in bicarbonate levels, glucose consumption and lactate production between the groups. The swirling effect with platelet-rich plasma samples decreased after 5 days of storage and after 7 days of storage for buffy coat samples. There was a significant twenty-fold increase in the mean IL-1β after 5 days of storage for both groups. Slight increases in IL-6 and IL-8 levels were seen at 5 days.ConclusionThe quality of platelet concentrates remained acceptable during 7 days of storage in respect to the swirling effect, pH and platelet activation. There were no significant differences between buffy coat-derived platelets and platelet-rich plasma in this study.

Platelets from platelet-rich-plasma versus buffy-coat-derived platelets: what is the difference?

“…In this issue of the journal, Costa et al present data on CD42b, CD62p (P-selectin), and cytokines IL-1b, IL-6, IL-8, and TNF-alpha. ( 6 ) In some respect, this study is similar to the earlier study by Metcalfe et al since P-selectin was measured in both studies. In contrast to the results of the study by Metcalfe et al, Costa et al did not observe higher levels of P-selectin in PRP PCs than in BC-PC.…”

Transfusion-associated adverse reactions (TAARs) and cytokine accumulations in the stored blood components: the impact of prestorage versus poststorage leukoreduction

“…These findings in RBC products were generally consistent with our results that cytokine levels of IL-1β, IL-8 and RANTES were significantly decreased by prestorage leukoreduction during the storage. In contrast, remarkable elevation of IL-1β, IL-6, IL-8 and TGF-β1 levels was seen during the process of storage in PCs [ 28 , 29 ]. Furthermore, it was reported that several cytokines, including RANTES, TGF-β1, IL-27, as well as soluble OX40 ligand (sOX40L), also a member of the TNF family and being associated with the inhibition of IL-6, IL-10 and IL-12 [ 30 ], were significantly increased during the storage of PHs [ 31 – 33 ].…”

Determinación de la viabilidad de los concentrados plaquetarios obtenidos de capa leucocitaria (buffy coat) durante siete días de almacenamiento

“…Otros estudios han demostrado que el índice de torbellino o swirling en concentrados derivados de capa leucocitaria disminuyó después del séptimo día, y que en concentrados obtenidos de plasma rico en plaquetas empezó a decrecer significativamente a partir del quinto día (20).…”