Electrical properties of the red blood cell membrane and immunohematological investigation

Fernandes, Heloise P.; César, Carlos L.; Barjas‐Castro, Maria de Lourdes

doi:10.5581/1516-8484.20110080

Cited by 159 publications

(108 citation statements)

References 21 publications

(30 reference statements)

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“…Fourteen (14) grounded subjects and fourteen (14) sham-grounded subjects participated in the one-hour study on yoga mats during which time they completed 5 twelve (12) minute sessions of ten (10) different yoga exercises. Table 2 t-test calculations show that the grounded group had a significant decrease in blood viscosity at both the systolic (p = 0.032) and diastolic (p = 0.031) measurements.…”

Section: Resultsmentioning

confidence: 99%

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Grounding the Human Body during Yoga Exercise with a Grounded Yoga Mat Reduces Blood Viscosity

Brown¹,

Chevalier²

2015

OJPM

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Objective: Research continues to show that being connected to the earth can increase the potential of the body to scavenge free radicals. This study examined the effect of just one hour of grounding on blood viscosity while subjects participated in gentle yoga exercises designed to initiate minor inflammation. Design: In this double blind model, twenty-eight (28) subjects met at the Bowerman Sports Medicine Clinic on the campus of the University of Oregon and were grounded to the earth via contact with a grounded yoga mat or were sham-grounded. Ten yoga exercises were repeated five times over a one-hour period. Blood was taken pre and post exercise and analyzed for blood viscosity using a scanning capillary viscometer. Results: Subjects connected to the earth significantly reduced their post exercise systolic blood viscosity (p = 0.03) and diastolic blood viscosity (p = 0.03). Conclusion: Grounding has the ability to affect exercise induced inflammation, thereby reducing blood viscosity.

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Section: Resultsmentioning

confidence: 99%

“…Four instructors were utilized in the study, and gave the same instructions on how to do each pose. Fourteen (14) subjects were tested on Day 1 and fourteen (14) were tested on Day 2 between the hours of 10:00 AM and 12:30 PM.…”

Section: Study Proceduresmentioning

confidence: 99%

Grounding the Human Body during Yoga Exercise with a Grounded Yoga Mat Reduces Blood Viscosity

Brown¹,

Chevalier²

2015

OJPM

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“…To assess this, we incubated P16 and P12 with human red blood cells (RBCs), which have a negatively charged membrane but no TNFRs nor intracellular organelles (Fernandes et al, 2011). After overnight culture, the membranes of the RBCs that had been incubated with P16 were ruptured, and hemoglobin was released into the supernatant (Fig.…”

Section: Cell Death Induced By Peptides Is Independent Of Tnfr1mentioning

confidence: 99%

Evolutionarily conserved primary TNF sequences relate to its primitive functions in cell death induction

Chen

Ying

et al. 2016

Journal of Cell Science

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TNF is a primitive protein that has emerged from more than 550 million years of evolution. Our bioinformatics study of TNF from nine different taxa in vertebrates revealed several conserved regions in the TNF sequence. By screening overlapping peptides derived from human TNF to determine their role in three different TNF-induced processes -apoptosis, necrosis and NF-κB stimulation -we found that TNF conserved regions are mostly related to cell death rather than NF-κB stimulation. Among the most conserved regions, peptides (P)12, P13 and P1213 (comprising P12 and P13) induced apoptosis, whereas P14, P15, P16 and P1516 (comprising P15 and P16) induced necrosis. Cell death induced by these peptides was not through binding to the TNF receptor. P16-induced necrosis was mainly through disruption of the cell membrane, whereas P1213-induced apoptosis involved activation of TRADD followed by formation of complex II. Finally, using a monoclonal antibody and a mutant TNF protein, we show that TNF-induced apoptosis is determined by a conserved linear sequence that corresponds to that within P1213. Our results reveal the determinant sequence that is key to the TNF primitive function of inducing apoptosis.

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“…Enzim proteolitik dapat digunakan untuk menghilangkan fragmen glikoprotein pada membran eritrosit sehingga meningkatkan kedekatan antar sel eritrosit dan meningkatkan akses antibodi terhadap antigen (Fernandes et al, 2011). Dengan alasan tersebut, uji aktivitas hemagglutinasi lektin dikerjakan tidak hanya terhadap eritrosit native, namun juga terhadap eritrosit yang diberi perlakuan enzim tripsin dan papain.…”

Section: Hasil Dan Bahasanunclassified

Penapisan Senyawa Hemagglutinin dari Makroalga Asal Pantai Binuangeun, Banten, Indonesia

Fajarningsih

Yamin

Yunita

et al. 2015

JPBKP

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ABSTRAKSebagai bagian dari penelitian penapisan lektin dari makroalga Indonesia, 17 esktrak protein makroalga yang dikoleksi dari Pantai Binuangeun, Banten telah diuji aktivitas hemagglutinasinya terhadap eritrosit kelinci dan eritrosit manusia golongan A, B, O, masing-masing dengan perlakuan enzim dan native. Ekstraksi dilakukan dengan menggunakan 2 jenis buffer, yaitu Phosphate Buffer Saline (PBS) dan Tris Buffer Saline (TBS) pH 7. Hasil penelitian menunjukkan bahwa pada beberapa sampel, ekstrak yang dihasilkan kedua buffer, menunjukkan aktivitas hemagglutinasi yang berbeda walaupun kadar total protein ekstrak makroalga yang diekstraksi dengan PBS dan TBS tidak berbeda. Sebagian besar ekstrak makroalga yang diuji mampu mengagglutinasi setidaknya satu jenis sel eritrosit yang digunakan. Secara umum, kelompok makroalga hijau memperlihatkan aktivitas hemagglutinasi yang lebih rendah dibandingkan kelompok makroalga merah dan coklat. Meskipun ekstrak Padina australis (makroalga coklat) memberikan hasil hemagglutinasi eritrosit kelinci negatif, namun ekstrak tersebut positif menghemagglutinasi eritrosit golongan darah B dan O. Di antara 8 makroalga hijau yang diuji, hanya dua sampel yang menunjukkan aktivitas hemagglutinasi, yaitu Chaetomorpha crassa dan Halimeda macroloba. Keempat ekstrak makroalga merah yang diuji menunjukkan aktivitas hemagglutinasi yang kuat terhadap eritrosit kelinci. Ekstrak makroalga merah Gracilaria lichenoides dan Gelidiella acerosa aktif terhadap semua jenis eritrosit uji. Sementara itu, hanya ekstrak Laurencia tronoi yang menunjukkan aktivitas hemagglutinasi terhadap eritrosit golongan darah A. KATA KUNCI:makroalga, lektin, hemagglutinasi ABSTRACT As part of lectins screening project from Indonesia's macroalgae, protein extracts from 17 macroalgae species collected from Binuangen Coast, Banten were examined for their hemagglutinating activity using native and enzyme-treated rabbit and human erythrocytes (A,B,O). Extraction of the macroalgae hemagglutinins was conducted using 2 buffers, e.g Phosphat buffer saline (PBS) and Tris buffer saline (TBS) pH

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Electrical properties of the red blood cell membrane and immunohematological investigation

Cited by 159 publications

References 21 publications

Grounding the Human Body during Yoga Exercise with a Grounded Yoga Mat Reduces Blood Viscosity

Grounding the Human Body during Yoga Exercise with a Grounded Yoga Mat Reduces Blood Viscosity

Evolutionarily conserved primary TNF sequences relate to its primitive functions in cell death induction

Penapisan Senyawa Hemagglutinin dari Makroalga Asal Pantai Binuangeun, Banten, Indonesia

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