2018
DOI: 10.5155/eurjchem.9.1.7-12.1661
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Abstract: (-)-Epigallocatechin gallate (EGCG) is a catechin found in green tea that has potential health benefits, such as anti-oxidant, anti-carcinogenic and anti-inflammatory effects. A rapid and sensitive Ultra-Performance Liquid Chromatographic (UPLC) method was developed and validated for the estimation of (-)-epigallocatechin-3-gallate in lipid-based formulation. The UPLC method was conducted on C18 analytical column (50 mm × 2.1 mm, 1.8 μm particle size). The mobile phase consisted of a mixture of acetic acid (1%… Show more

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Cited by 5 publications
(2 citation statements)
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“…Therefore, the LOD was calculated as 0.170 μg/mL, and the LOQ was 0.515 μg/mL. The limits in the present study were found to be lower than the previous study, which reports LOD as 0.528 μg/ mL and LOQ as 1.600 μg/mL, thus suggesting the present method was more sensitive [13]. Similarly, the LOD and LOQ with regard to the green tea leaf extract were calculated.…”
Section: System Suitability and Methods Precisioncontrasting
confidence: 53%
See 1 more Smart Citation
“…Therefore, the LOD was calculated as 0.170 μg/mL, and the LOQ was 0.515 μg/mL. The limits in the present study were found to be lower than the previous study, which reports LOD as 0.528 μg/ mL and LOQ as 1.600 μg/mL, thus suggesting the present method was more sensitive [13]. Similarly, the LOD and LOQ with regard to the green tea leaf extract were calculated.…”
Section: System Suitability and Methods Precisioncontrasting
confidence: 53%
“…Previously, methods have been developed to estimate the EGCG using Spectrophotometry [7], High Performance Liquid Chromatography (HPLC) [8,9], Reverse Phase (RP) HPLC [10,11] and Ultra-Performance Liquid Chromatography (UPLC) [12,13], Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) [14], Ultra-Performance Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (UPLC/ESI-MS), Ultra-Performance Liquid Chromatography-Diode Array Detector-Mass Spectroscopy (UPLC-DAD-MS) [15] and Ultra-Performance Liquid Chromatography-Time-of-Flight-Mass Spectrometry (UPLC-TOF-MS) [16]. Also keeping in view of the low bioavailability of the compound and the limited studies to identify EGCG alone from the samples, there is a strong need to develop and validate a simple and rapid method to identify EGCG.…”
Section: Introductionmentioning
confidence: 99%