Determination of metformin hydrochloride in human plasma by UPLC/MS/MS: Application in bioequivalence study

Mohammed, Samah A.; Eldardiri, Mohammed; Elhabak, Mona; Zaid, Khaled Ibrahim Abu

doi:10.5155/eurjchem.6.2.178-182.1234

Cited by 1 publication

(1 citation statement)

References 8 publications

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“…Another validated method using UPLC had been done by Mohammed [15] using Waters UPLC Xevo TQD (Milford, Massachusetts, USA) using the 1.38 μm 2.1 × 50 mm HSS T3 Acquity UPLC column at 40° C. In previous study of selectivity was found that there is no endogenous peaks which can interfere the quantification of metformin and internal standard. It is known that use of UPLC is preferred because of fewer eluent use, fewer samples, shorter retention times so that analysis can be performed effectively and efficiently.…”

Section: Discussionmentioning

confidence: 99%

A Validated Ultra Performance Liquid Chromatography Method for Quantification of Metformin in Human Plasma

Yunivita

Dewi

Setiabudiawan

et al. 2018

J. Pure App. Chem. Res.

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Metformin is oral hypoglycemic or blood sugar-lowering drug which is used for the first-line drug in the treatment of diabetes mellitus type 2. This study presented the validated of Ultra Performance Liquid Chromatography UltraViolet (UPLC-UV) method for the determination of metformin in human plasma. Metformin levels were measured using UPLC with UV detector and liquid-liquid extraction method. Separation was carried out on a Acquity UPLC HSS T3 100mm × 2.1mm i.d. column (1.8μm partikel size) using gradient elution of acetonitrile and phosphate buffer 0.02 M (0.6 mL/min) as mobile phase at 30°C. The analyte was monitored at 236 nm. No endogenous substances were found to interfere with the peaks of drug and internal standard. The value of percent deviation and the coefficient variation obtained respectively less than the percentage set in the FDA guidelines. The linearity factor values were more than 0.997 and LOD was 0.01µg/mL. UPLC with UV detector is able to analyze metformin in a short time with good precision and accuracy which is useful for bioequivalence and bioavailability studies.

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Section: Discussionmentioning

confidence: 99%