Pesquisa de anticorpos anti-Neospora caninum em bovinos leiteiros, cães e trabalhadores rurais da região Sudoeste do Estado de Mato Grosso

Abstract

ResumoConsiderando a importância da neosporose interferindo na produtividade animal, avaliou-se a frequência de anticorpos anti-Neospora caninum em amostras de soros de bovinos leiteiros da Região Sudoeste do Estado de Mato Grosso, Brasil, complementando-se com amostras sorológicas colhidas de cães e de humanos que conviviam nas mesmas propriedades rurais amostradas. Um total de 1.036 amostras de soros foram analisadas, sendo 932 de fêmeas bovinas leiteiras, 37 de cães e 67 de humanos, provenientes de 24 propriedades e examinados por meio da reação de imunofluorescência indireta (RIFI). As amostras de soros humanos reagentes foram testadas novamente por Western-blotting para confirmação dos resultados. Anticorpos anti-N. caninum foram encontrados em 499 bovinos (53,5%), em pelo menos um animal positivo por propriedade, em 25 caninos (67,6%) e em sete humanos (10,5%). Não houve diferença significativa no número de bovinos positivos por faixa etária. Os resultados obtidos indicam uma ampla disseminação de N. caninum na região estudada.Palavras-chave: Neospora caninum, soroprevalência, bovinos, caninos, humanos. AbstractConsidering the importance of neosporosis in the animal health and production, the frequency of antibodies to Neospora caninum was evaluated in dairy cattle of the Southwestern region of Mato Grosso State, Brazil, in addition to serum samples obtained from dogs and humans living in the farms. A total of 1036 serum samples were analyzed, from which 932 were from dairy bovine females, 37 from dogs and 67 from humans, from 24 farms and examined by the indirect fluorescent antibody test (IFAT). Reactive human serum samples were retested by Western-blotting to confirm the results. Antibodies to N. caninum were found in 499 cattle sera (53.5 %), with at least one positive in each farm, 25 dog sera (67.6 %) and seven human sera (10.5 %). There was no significant difference in the number of positive cattle sera according to age group. The results indicate a wide dissemination of N. caninum in the studied region.

Serological detection of Toxoplasma gondii, Leishmania infantum and Neospora caninum in cats from an area endemic for leishmaniasis in Brazil

“…The importance of N. caninum as a zoonotic agent remains unknown, but N. caninum antibodies have been detected in humans in the United States (TRANAS et al, 1999) and Brazil (LOBATO et al, 2006;BENETTI et al, 2009). In Brazil, IgG antibodies against N. caninum were detected in patients infected with human immunodeficiency virus (HIV) and in patients with neurological disorders, suggesting the possibility that neosporosis could be an opportunistic parasite in immunocompromised patients (LOBATO et al, 2006).…”

All about neosporosis in Brazil

“…In the second study, antibodies to N. caninum (IFAT,1:50) were sought in Human Immunodeficiency Virus (HIV) patients; which 26.1% (81/310) patients from MS and 31.2% (10/31) patients from PR were seropositive and one patient had a titer of 1:400 (OSHIRO et al, 2015). In the third report, N. caninum antibodies (IFAT 1:100) were detected in 7 of 67 (10,5%) humans from 24 farms of MT (BENETTI et al, 2009). …”

“…Even after using a higher cut-off titer (IFAT 1:200) than used most of the surveys listed in Table 4, high prevalence were recorded from MG, where N. caninum antibodies were detected in 23 of 24 herds with individual seroprevalence of 21.6% ; and MT, where antibodies to N. caninum were found in 499 (53.5%) of 932 cattle samples, with at least one positive in each farm (BENETTI et al, 2009). In another report from MG, very high (91.2%; 510/559) prevalence of antibodies to N. caninum was found among 18 farms (GUEDES et al, 2008), revealing that N. caninum is well spread in the southern region of the state.…”

Neospora caninum: Relación Biológica con Toxoplasma gondii y su Potencial como Zoonosis

“…Tres estudios son los que hasta el momento evidencian exposición de N. caninum en humanos; muestras positivas serológicamente mediante ELISA, IFAT y western blot de pacientes con VIH, con signos neurológicos o con factores predisponentes como es el vínculo directo con producciones bovinas y contacto con caninos en zonas rurales (19,20,23).…”

“…Three studies so far show exposure of N. caninum in humans; serologically positive samples by means of ELISA, IFAT and western blot in patients with HIV, with neurological signs or with predisposing factors such as a direct link with bovine production and contact with dogs in rural areas (19,20,23).…”