Molecular diagnosis of Anaplasmataceae organisms in dogs with clinical and microscopical signs of ehrlichiosis

Dagnone, Ana Silvia; Souza, Alda Izabel de; André, Marcos Rogério; Machado, Rosangela Zacarias

doi:10.4322/rbpv.01804004

Cited by 39 publications

(52 citation statements)

References 44 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The occurrence (5.47%) of coinfections in dogs caused by A. platys and E. canis reinforces the hypothesis that these infections are probably transmitted by the same vector; similar results were observed in different geographical regions in Brazil (DANTAS-TORRES, 2008;DAGNONE et al, 2009;RAMOS et al, 2009;SANTOS et al, 2009). This phenomenon was also described in dogs from Thailand (SUKSAWAT et al, 2001b), Venezuela (SUKSAWAT et al, 2001a;HUANG et al, 2005), and Grenada (YABSLEY et al, 2008).…”

Section: Resultssupporting

confidence: 72%

“…However, these results were higher than those observed in Rio de Janeiro, State of Rio de Janeiro, 15.84% (FERREIRA et al, 2007), Jaboticabal, State of Sao Paulo, 8% (DAGNONE et al, 2009), and Ribeirao Preto, State of Sao Paulo, 14.9% (SANTOS et al, 2009). Moreover, prevalence levels of A. platys in the USA, Venezuela, Italy, and Grenada varied from 4% to 55% (KORDICK et al, 1999;HUANG et al, 2005;DE LA FUENTE et al, 2006;YABSLEY et al, 2008).…”

Section: Resultscontrasting

confidence: 52%

“…When the result (19.4%) of A. platys was compared with similar studies, it was possible to observe that the prevalence level derived from this study was lower than that described in Campo Grande, State of Mato Grosso do Sul, 42% (DAGNONE et al, 2009), and Recife, State of Pernanbuco, 55% (RAMOS et al, 2009). However, these results were higher than those observed in Rio de Janeiro, State of Rio de Janeiro, 15.84% (FERREIRA et al, 2007), Jaboticabal, State of Sao Paulo, 8% (DAGNONE et al, 2009), and Ribeirao Preto, State of Sao Paulo, 14.9% (SANTOS et al, 2009).…”

Section: Resultsmentioning

confidence: 84%

“…Since there is a common vector for both diseases, coinfections in animals are frequent (KORDICK et al, 1999;YABSLEY et al, 2008;DAGNONE et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The identification of hemoparasites in blood smears is the most widely used technique in clinical practice to characterize morulae in leukocytes and platelets, but this method has low diagnostic sensitivity and specificity and must be supplemented with the use of molecular techniques, such as PCR (NAKAGHI et al, 2008;DAGNONE et al, 2009;RAMOS et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

Silva

Benitez

Girotto

et al. 2012

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced by Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence of E. canis and A. platys in blood samples collected from 256 domiciled dogs in the municipality of Jataizinho, located in north region of the State of Parana, Brazil, by PCR assay. The occurrence of E. canis and A. platys was 16.4% (42/256) and 19.4% (49/256), respectively; while 5.47% (14/256) of the dogs evaluated were co-infected by these two organisms. The presence of E. canis and A. platys was not significantly associated with the variables evaluated (sex, age, outdoor access, and presence of ticks during blood collection). Infection of dogs by E. canis was associated with anemia and thrombocytopenia, while infection induced by A. platys was related only to thrombocytopenia. Canine monocytic ehrlichiosis and canine thrombocytic anaplasmosis should be included in the differential diagnoses when these hematological alterations are observed during routine laboratory evaluation of dogs.Keywords: Canine monocytic ehrlichiosis, canine thrombocytic anaplasmosis, PCR, anemia, thrombocytopenia. ResumoErliquiose monocítica canina, causada principalmente por Ehrlichia canis, e anaplasmose trombocítica canina, devida a infecção com Anaplasma platys, são importantes doenças transmitidas por carrapatos que acometem os cães, com evidências que podem também acometer o homem. O presente estudo avaliou a ocorrência desses agentes em amostras de sangue de 256 cães domiciliados na cidade de Jataizinho, na região Norte do Paraná, Brasil, utilizando a técnica da Reação em Cadeia da Polimerase (PCR). A ocorrência de E. canis e A. platys foi de 16,4% (42/256) e 19,4% (49/256), respectivamente, com 5,47% (14/256) dos animais apresentando coinfecção. Não foi observada associação significativa com as variáveis sexo, idade, acesso à rua e presença de carrapatos no momento da coleta de sangue. A infecção por E. canis teve relação com anemia e com trombocitopenia, enquanto a infecção por A. platys apresentou relação apenas com trombocitopenia. Com base nos resultados obtidos, reforçou-se a necessidade de que erliquiose e anaplasmose canina devem estar entre os diagnósticos diferenciais, quando da detecção de anemia e trombocitopenia em exames laboratoriais.

show abstract

Section: Resultssupporting

confidence: 72%

Section: Resultscontrasting

confidence: 52%

Section: Resultsmentioning

confidence: 84%

“…Since there is a common vector for both diseases, coinfections in animals are frequent (KORDICK et al, 1999;YABSLEY et al, 2008;DAGNONE et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations