Prevalence and molecular characterization of Anaplasmataceae agents in free-ranging Brazilian marsh deer (Blastocerus dichotomus)

Sacchi, Ana Beatriz Vieira; Duarte, José Maurício Barbanti; André, Marcos Rogério; Machado, Rosângela Zacarias

doi:10.1016/j.cimid.2012.02.001

Cited by 56 publications

(47 citation statements)

References 55 publications

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“…and Anaplasma spp. may be present in wild carnivores , cats (BRAGA et al, 2012;ANDRÉ et al, 2014), wild birds , and wild deer (MACHADO et al, 2006;SACCHI et al, 2012). These data indicate the possibility of agents showing similar genotypes to E. chaffeensis and E. canis being involved in the positivity of sampled dogs.…”

Section: Discussionmentioning

confidence: 83%

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

Guedes

Oliveira

Carvalho

et al. 2015

Rev. Bras. Parasitol. Vet.

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Ehrlichiosis is a zoonotic disease that is caused by bacteria of the genus Ehrlichia. The aims of this study were to detect the presence of Ehrlichia spp. in the blood of dogs in Ituberá, Bahia, and to compare the sensitivities and specificities of blood smear, serological, and molecular examinations. Furthermore, this study identified factors associated with exposure to the agent in dogs in this locality. Blood samples were collected from 379 dogs and submitted for indirect immunofluorescent assay and polymerase chain reaction testing for the detection of Ehrlichia spp. antibodies and DNA, respectively. Additionally, a peripheral blood smear was obtained from the ear tip for parasite identification. Of the 379 animals, 12.4%, 32.7%, and 25.6% were identified as positive on the blood smear, serological, and molecular tests, respectively. The dogs positive in one of the three techniques were considered exposed (46.9%). Younger dogs and rural habitat were protective factors and presence of ticks and contact with other dogs were the risk factors associated with exposure to the agent. It was concluded that dogs of Ituberá have high positivity for Ehrlichia spp. and that the diagnostic methods used for detection are complementary.Keywords: Diagnosis, dog, Ehrlichia canis, Indirect Immunofluorescence, nested PCR. ResumoErliquiose é uma doença zoonótica causada por bactérias do gênero Ehrlichia. O objetivo desse estudo foi detectar a presença de Ehrlichia spp. no sangue de cães em Ituberá-BA, e comparar as sensibilidades e especificidades do esfregaço sanguíneo, e testes sorológico e molecular. Além disso, esse estudo identificou fatores associados com a exposição ao agente em cães desta localidade. Amostras de sangue foram coletadas de 379 cães e submetidas à Reação de Imunofluorescência Indireta e Reação em Cadeia de Polimerase para detecção de anticorpos e DNA de Ehrlichia spp., respectivamente. Adicionalmente, sangue periférico de ponta de orelha foi coletado para identificação do parasita. Dos 379 animais, 12,4%, 32,7% e 25,6% foram identificados como positivos no esfregaço sanguíneo, teste sorológico e molecular, respectivamente. Cães positivos em uma das três técnicas foram considerados expostos (46,9%). Cães mais novos e hábitat rural foram fatores de proteção e presença de carrapatos e contato com outros cães foram os fatores de risco associados à exposição ao agente. Foi concluído que, os cães do município de Ituberá têm alta positividade para Ehrlichia spp. e que os diferentes métodos diagnósticos utilizados para sua detecção são complementares.

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Section: Discussionmentioning

confidence: 83%

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

Guedes

Oliveira

Carvalho

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Melo et al (2011), studying this same dogs, reported greater frequency of seropositive dogs as they become older, probably due to a higher chance of becoming infested with ticks over time. Despite the recent reports of ehrlichial agents other than E. canis in Brazil (MACHADO et al, 2006;SILVEIRA et al, 2012;ANDRÉ et al, 2010ANDRÉ et al, , 2012SACCHI et al, 2012), molecular detection of new genotypic variants in Brazilian wildlife animals has been reported, since those animals play the role of sentinels for vector-borne pathogens because they can act as hosts for both bacteria and arthropod, including wildlife of the Pantanal region (WIDMER et al, 2011). However, the present work failed to detect Ehrlichia species different from E. canis infecting dogs from northern Pantanal, even though we used a high sensitive PCR capable of detecting all known Ehrlichia species .…”

Section: Resultsmentioning

confidence: 99%

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

Santos

Melo

Moraes‐Filho

et al. 2013

Rev. Bras. Parasitol. Vet.

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The present study evaluated the presence of Ehrlichia DNA in the blood samples of 320 dogs from the urban and rural areas of the municipality of Poconé, Pantanal region, Mato Grosso state, by Polymerase Chain Reaction (PCR), targeting the ehrlichial dsb gene. Risk factors for infection in dogs were also evaluated. Forty-eight (15%, 95% CI: 11.4-19.5%) dogs were positive: 25 (15.6%, 95% CI: 10.4-22.2%) from the urban area and 23 (14.4%, 95% CI: 9.3-20.8%) from the rural area (P > 0.05). Partial DNA sequence obtained from PCR products of 18 samples from the urban area and 16 samples from the rural area were 100% identical to E. canis from Brazil and the USA. This study reports the first E. canis molecular detection in dogs from the northern Pantanal region.Keywords: Dogs, tick borne disease, Ehrlichia canis, Anaplasmataceae, Poconé, Brazil. ResumoO presente estudo avaliou a presença de DNA de Ehrlichia spp. em 320 cães das áreas urbana e rural do município de Poconé, região do Pantanal de Mato Grosso, pela PCR visando o gene dsb. Os fatores de risco para a infecção em cães também foram avaliados. Quarenta e oito (15%, IC 95%: 11,4-19,5%) cães foram positivos, 25 (15,6%, IC 95%: 10,4-22,2%) da área urbana e 23 (14,37%, 95% CI: 9,3-20,8%) da área rural (P > 0,05). Sequências parciais de DNAs obtidos a partir de produtos da PCR de 18 amostras da área urbana e 16 da área rural foram 100% idênticas a E. canis do Brasil e EUA. Este estudo relata a primeira detecção molecular de E. canis em cães da região norte do Pantanal.

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“…No Brasil, já foram encontradas as espécies A. platys, A. phagocytophilum, A. bovis (Sacchi et al 2012), A. marginale (Picoloto et al 2010), E. canis, E. chaffeensis e E. ewingii, todas tendo como vetor o carrapato (Vieira et al 2011). A espécie N. risticii, descrita infectando equinos no Rio Grande do Sul, tem como vetores trematódeos aquáticos (Coimbra et al 2006).…”

Section: Introductionunclassified

Investigação molecular de Ehrlichia canis, Anaplasma platys, Anaplasma phagocytophilum e Rickettsia spp. em felídeos selvagens cativos

et al. 2018

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RESUMO.-Doenças transmitidas por vetores estão emergindo e reemergindo em todo o mundo, representando um desafio na medicina humana e veterinária. Entre essas doenças estão aquelas causadas pelos agentes da ordem das Rickettsiales, que são bactérias Gram-negativas intracelulares obrigatórias, com capacidade de infectar vários animais e seres humanos. Vector-borne diseases have been emerging and reemerging all over the world, causing a challenge to veterinary and human medicine. Among these diseases are those caused by agents of the order Rickettsiales, obligatory intracellular Gram-negative bacteria, with ability to infect several animals and humans. Rickettsiales of the species Ehrlichia spp. and Anaplasma spp. residing in cytoplasmic vacuoles of leukocytes and platelets. Rickettsiales of the species Rickettsia spp. freely infect cytoplasm or nucleus of host cells. The aim of the present study was to investigate the natural infection with Ehrlichia canis, Anaplasma platys, Anaplasma phagocytophilum and Rickettsia spp. in captive wild felids at the Federal District and Goiás, Brazil. In addition, it was also aimed to relate possible changes in hemogram with the presence of these agents. Blood samples from 34 animals were analyzed by PCR to detect the presence of DNA from these agents. The DNA of Ehrlichia canis was detected in 5.8% (2/34) of samples. A. platys was detected in 64.7% (22/34), A. phagocytophilum was detected in 5.8% (2/34). The DNA of Rickettsia spp. was not detected in any sample. Two felides presented co-infection with E. canis and A. platys, and two presented co-infection with A. platys and A. phagocytophilum. There were no significant differences in hematological data from positive and negative samples. The data suggest that captive wild felids can serve as potential reservoirs for Ehrlichia spp. and Anaplasma spp., despite hematological abnormalities were not observed. As Rickettsiales das espécies

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Prevalence and molecular characterization of Anaplasmataceae agents in free-ranging Brazilian marsh deer (Blastocerus dichotomus)

Cited by 56 publications

References 55 publications

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

Canine ehrlichiosis: prevalence and epidemiology in northeast Brazil

Molecular detection of Ehrlichia canisin dogs from the Pantanal of Mato Grosso State, Brazil

Investigação molecular de Ehrlichia canis, Anaplasma platys, Anaplasma phagocytophilum e Rickettsia spp. em felídeos selvagens cativos

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