Streptococcus Colonial Growth of Dental Plaque Inhibition Using Flavonoid Extract of Ants Nest (Myrmecodia pendans): An in Vitro Study

Abstract

Objective: To know the activity of resistance of flavonoid content in ant nest plant in decreasing the number of colonies S. mutans oral cavity of children as a medic herbal material. Material and Methods: The subjects were plaque sample of 20 children aged 7-12 years. Research begins by making toothpaste from ant nest extract. Samples of children's dental plaque were inserted into BHIB media, after which incubated for 24 hours, 1/10 dilution with BHIB media three times, followed by TYC media planting and incubation of anaerob with temperature 37 0 C for 48 hours. After that then count the number of colonies of S. mutans. Results: On ethyl acetate extract of ant nest incubated at room temperature with concentration 20%, 40%, 60%, 80%, 100% obtained a decrease from each treatment amount of Streptococcus mutans colony on TYC media with median value of each treatment was 89, 67, 64, 61, 59 and 51 for the ethyl acetate fraction, and 62, 61, 60, 59, 49 at the ethanol fraction. There was no significant difference between the six concentration groups (p>0.05). Conclusion: Flavonoids extract of ant nest plants have growth barrier on Streptococcus mutans bacteria, the greater the concentration given the greater the number of S. mutans colony.

Pengaruh Konsentrasi Ekstrak Kulit Buah Naga Merah terhadap Pertum-buhan Streptococcus mutans pada Sediaan Obat Kumur (Uji Invitro)

“…Enzim GTF yang terganggu mengakibatkan protein bakteri menjadi nonaktif dan proses demineralisasi gigi menjadi terhambat. 34 Interaksi yang ditimbulkan oleh protein bakteri dengan senyawa flavonoid membentuk koagulasi protein pada dinding bakteri dan meningkatkan tekanan osmotik di dalam sel bakteri yang mencapai 5-20 atm. 35,36 Peningkatan tekanan osmotik ini mampu memicu terjadinya kerusakan pada dinding sel bakteri hingga menyebabkan lisis.…”

“…35,36 Peningkatan tekanan osmotik ini mampu memicu terjadinya kerusakan pada dinding sel bakteri hingga menyebabkan lisis. 34 Obat kumur ekstrak kulit buah naga merah konsentrasi 50% memiliki kemampuan menghambat pertumbuhan Streptococcus mutans yang lebih efektif dan menghasilkan zona hambat yang lebih luas daripada obat kumur dengan konsentrasi ekstrak 25%, 12,5%, dan 6,25%. Kadar hambat minimum dihasilkan oleh obat kumur konsentrasi ekstrak 6,25%, yaitu pada konsentrasi terkecil tersebut masih dapat menghambat pertumbuhan Streptococcus mutans.…”

Solution Inhibitory of Ant Nest (Myrmecodia Pendans) Extract on the Formation of Plaque in Children with Early Childhood Caries

“…Ant nests are very rich in nutrients, including calcium, iron, protein, vitamin A, vitamin B, and vitamin C. Ant nests contain higher iron than other vegetables, which is 17.2 mg / 100 g [6,8]. In addition, ant nests also contain various kinds of amino acids, including amino acids in the form of aspartic acid, glutamic acid, alanine, valine, leucine, isoleucine, histidine, lysine, arginine, phenylalanine, tryptophan, cysteine, and methionine [5,11].…”

Efektivitas Ekstrak Etanol Umbi Sarang Semut Jenis Myrmecodia pendens Terhadap Daya Hambat Bakteri Porphyromonas gingivalis (Studi In Vitro)

“…Semakin tinggi konsentrasi ekstrak etanol sarang semut maka semakin luas zona hambat yang terbentuk. 6,7 Maka peneliti tertarik untuk melakukan penelitian mengenai efektivitas ekstrak etanol umbi sarang semut dengan konsentrasi 25% dan 50% terhadap daya hambat bakteri Porphyromonas gingivalis. anaerob, berpigmen hitam, dan berbentuk batang.…”