&lt;b&gt;Population structure and impact of recurrent selection on popcorn using EST-SSR markers

Guimarães, Amanda Gonçalves; Júnior, Antônio Teixeira do Amaral; Filho, Janeo Eustáquio de Almeida; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga

doi:10.4025/actasciagron.v40i1.35218

Cited by 8 publications

(12 citation statements)

References 24 publications

(41 reference statements)

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“…The amplified product size was separated by gel electrophoresis in 4% high-resolution agarose (Guimaraes et al 2018) gel stained in ethidium bromide solution in 1× TAE buffer at a constant 80 V current for 4 h. To visualize the bands, gel was observed in UV light provided by a trans-illuminator in (Syngene G: Box) gel documentation system. The size and molecular weight of the amplification products was estimated by a standard 50 bp ladder (Fermentas Thermofisher Pvt.…”

Section: Separation and Detection Of Amplified Pcr Productsmentioning

confidence: 99%

Assessment of morphological and genetic variability through genic microsatellite markers for essential oil in Sandalwood (Santalum album L.)

et al. 2019

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Sandalwood (Santalum album L; family Santalaceae) is a highly significant aromatic oil yielding tree. It is valued for two important traits, heartwood and essential oil obtained from the heartwood. This study was proposed to assess the morphological and genetic variability of sandalwood accessions. For this, genotypes were randomly selected (n = 177) from the 14 populations from three states in southern India. The total heartwood oil content and quality was estimated by UV method and GC-MS. Total 14 oil-specific genic SSR markers were procured to evaluate the genetic diversity among the sandalwood accessions. Total core size, heartwood content, and oil of S. album ranged from 4.4 to 19.1 cm; 0.0 to 17.3 cm; and 0.0 to 5.96% with covariance 27.61, 85.25, and 73.12% followed by mean 9.74, 3.77, and 2.71, respectively. Genetic diversity estimates were highly polymorphic in terms of Na 7.28, Ne 5.89, He 8.0 PIC 0.891, with little Ho, and F-0.922. AMOVA revealed that minimal genetic variation among populations and highest variation was found among individuals with Nm (58.4). The UPGMA reveals the cluster favored the grouping pattern by the PCA analysis. Structure and PCA analysis clustered the entire populations into two major groups with F ST 0.046 in which population of Kerala and Karnataka were pure and Telangana accessions were found admixtures. No significant correlation (r 2 = 0.23, P = 0.00) was observed between heartwood oil and genetic structures. A high degree of transferability of genic markers would facilitate the assessment of novel genotypes for future tree improvement and conservation of Sandalwood populations.

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Section: Separation and Detection Of Amplified Pcr Productsmentioning

confidence: 99%

Assessment of morphological and genetic variability through genic microsatellite markers for essential oil in Sandalwood (Santalum album L.)

et al. 2019

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“…Since its inclusion in the germplasm bank of the State University of Northern Rio de Janeiro (UENF), the UENF-14 popcorn population has been subject to a series of recurrent intrapopulational selection cycles targeting the improvement of economically important traits and the maintenance of the population variability. Until the eighth cycle, only phenotypic evaluations were performed [12–20]. The population is currently in the ninth selection cycle (C-9), and the introduction of genotyping proved important for predicting gains for the main traits of interest as well as for allowing a more detailed study of the genomic regions responsible for such traits.…”

Section: Introductionmentioning

confidence: 99%

SNP-based mixed model association of growth- and yield-related traits in popcorn

Mafra¹,

Júnior²,

Filho³

et al. 2019

PLoS ONE

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The identification of the genes responsible for complex traits is highly promising to accelerate crop breeding, but such information is still limited for popcorn. Thus, in the present study, a mixed linear model-based association analysis (MLMA) was applied for six important popcorn traits: plant and ear height, 100-grain weight, popping expansion, grain yield and expanded popcorn volume per hectare. To this end, 196 plants of the open-pollinated popcorn population UENF-14 were sampled, selfed (S 1 ), and then genotyped with a panel of 10,507 single nucleotide polymorphisms (SNPs) markers distributed throughout the genome. The six traits were studied under two environments [Campos dos Goytacazes-RJ (ENV1) and Itaocara-RJ (ENV2)] in an incomplete block design. Based on the phenotypic data of the S 1 progenies and on the genetic characteristics of the parents, the MLMA was performed. Thereafter, genes annotated in the MaizeGDB platform were screened for potential linkage disequilibrium with the SNPs associated to the six evaluated traits. Overall, seven and eight genes were identified as associated with the traits in ENV1 and ENV2, respectively, and proteins encoded by these genes were evaluated for their function. The results obtained here contribute to increase knowledge on the genetic architecture of the six evaluated traits and might be used for marker-assisted selection in breeding programs.

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“…These results indicate that even with the use of different progeny strategies (full-sibs, S 1 and halfsibs), there was an increase in allele frequencies for the traits of interest, with greater adaptability to counties in the north and northwest of the State of Rio de Janeiro. This progress has been described by Ribeiro et al (2016) and Guimarães et al (2018b). While the first authors investigated cumulative gains in GY and PE based on phenotypic responses in the same study population over seven cycles (C0-C6), the latter authors used microsatellite molecular markers (EST-SSRs), inferring an increase in favourable alleles over nine selection cycles (C0-C8), which may be related to the main traits of interest.…”

Section: Genetic Progress In the Selection Cycles Of Population Uenf-14mentioning

confidence: 99%

Genetic Gains in the Popcorn Population Uenf-14: Developing the Ninth Generation of Intrapopulation Recurrent Selection

et al. 2019

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The occurrence of negative correlations between grain yield (GY) and popping expansion (PE) in popcorn complicates the selection process. In an attempt to overcome this inconvenience, we proposed the use of the importance characteristic volume per popcorn per hectare (PV). The objectives of this study were to develop the ninth cycle of the UENF-14 popcorn population, to estimate the direct and indirect effects of PV and to verify the genetic progress among all selective cycles. We obtained and evaluated 200 inbred progenies in randomised blocks arranged in replicates within sets, with nine sets consisting of 25 progenies and one set with all previous eight cycles with three replicates. The average height of the plants, prolificacy, 100-grain weight, grain yield, grain popping expansion and volume per popcorn per hectare were evaluated. Track analysis was performed to determine the direct and indirect effects, and the Mulamba and Mock selection index was calculated for the selection of the 40 superior progenies. There was genetic variability among progenies in all evaluated traits, with a cause-and-effect relationship between PV with GY and PE, allowing simultaneous gains with indirect selection. The selection of the best progenies was more effective when using arbitrarily assigned weights, which provided higher predicted gains for PV (20.73%). The means obtained in all cycles indicated increases, demonstrating the efficiency of using continuous recurrent selection in popcorn breeding programs.

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<b>Population structure and impact of recurrent selection on popcorn using EST-SSR markers

Cited by 8 publications

References 24 publications

Assessment of morphological and genetic variability through genic microsatellite markers for essential oil in Sandalwood (Santalum album L.)

Assessment of morphological and genetic variability through genic microsatellite markers for essential oil in Sandalwood (Santalum album L.)

SNP-based mixed model association of growth- and yield-related traits in popcorn

Genetic Gains in the Popcorn Population Uenf-14: Developing the Ninth Generation of Intrapopulation Recurrent Selection

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