<b>Morphoanatomy and physiology of Pouteria gardneriana Radlk plantlets grown in vitro at varied photosynthetic photon flux densities

Abstract

ABSTRACT. Micropropagation is an important tool for the multiplication of native Cerrado species. However, understanding the responses of these species under in vitro culture conditions is still incomplete. Thus, the present study aimed to analyze the growth, anatomical behavior and physiology of Pouteria gardneriana cultivated in vitro under photoautotrophic conditions. Nodal segments were cultured at photosynthetic photon flux densities (PPFD) of 75, 100 and 150 μmol m de sacarose. Após 60 dias de cultivo in vitro observou-se regeneração de plântulas de P. gardneriana apenas quando a sacarose estava presente no meio de cultivo. Maior massa fresca e seca, maior espessura do parênquima paliçádico e diâmetro polar e equatorial foram observadas nas plântulas cultivadas no PPFD de 150 μmol m -2 s -1 . As diferentes PPFD utilizadas neste estudo foram suficientes para compreender o comportamento desta espécie in vitro.Palavras-chave: Cerrado, fotoautotrofismo, Sapotaceae.

Leaf morphoanatomy of an endemic massaranduba from Chapada Diamantina, Bahia, Brazil

“…The mesophyll with dorsiventral disposition found in P. andarahiensis corroborates the description of Metcalfe & Chalk (1957) for the family and with the data found by Monteiro et al (2007), Almeida Jr. (2013; Leite et al (2017) and Lima et al (2019), for other species of Sapotaceae. It is possible to visualize sclerenchyma columns in the mesophyll, these columns together with the thick cuticle, may be related to long periods of drought, strong light and poor nutrient soil (Dickison 2000;Cutler et al 2011).…”

“…Regarding the diagnostic characters of the family, trichomes are numerous in young leaves of P. andarahiensis, and according to Press (1999) in hot and arid environments, the main role of trichomes is to decrease leaf temperature and water loss, decreasing the absorption of solar radiation, and hypoestomatic leaves with anisocytic stomata are in accordance with several works for the family and genus (Solereder 1908;Metcalfe & Chalk 1957;Pennington 2004;Monteiro et al 2007;Leite et al 2017), these stomata are numerous, which Wilkinson (1979) relates to xerophyte species.…”