Estabelecimento de protocolo para multiplicação in vitro de Bastão-do-imperador (Etlingera elatior) Jack RM Sm.

Colombo, Larissa Abgariani; Assis, Adriane Marinho de; Faria, Ricardo Tadeu de; Roberto, Sérgio Ruffo

doi:10.4025/actasciagron.v32i4.4830

Cited by 5 publications

(3 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lobster Salmón, obtained the best multiplication rate (3.0 shoots/explant) with a combination of 2.0 mg L -1 BAP + 0.5 mg L -1 IAA at 28 days of in vitro growth. Colombo et al (2010), working with Etlingera elatior (Jack) R.M. Sm, obtained an average of 3.70 shoots/explant in a culture medium containing 3.37 mg L -1 NAA and 4.5 mg L -1 BAP after 90 days of growth.…”

Section: Resultsmentioning

confidence: 99%

“…A similar result was obtained by Nathan et al (1992), who found a lack of shoot proliferation in Heliconia psittacorum L. f. when BAP was removed from the culture medium. According to Colombo et al (2010), with in vitro cultivation, the use and combination of growth regulators is necessary to compensate for deficiencies in the endogenous hormone levels of the shoots, since these are isolated from the mother plant. The addition of BAP to the culture medium favoured the emission of shoots in the explants.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Rodrigues

Santos

Carvalho

2016

View full text Add to dashboard Cite

Considering the growing economic importance of tropical flowers and the advantages of techniques applied to the in vitro cultivation of these plants, it is necessary to carry out studies to evaluate growth in species such as Heliconia latispatha. The aim of this study therefore, was to evaluate in vitro shoot induction for different concentrations of BAP and NAA and as a function of the photoperiod. Explants from zygotic embryos were inoculated in MS medium containing different concentrations of BAP (0.0, 0.5, 1.0, 1.5, 2.0 or 2.5 mg L -1 ), with the cultures kept in a growth room at a temperature of 24.0 ± 2.0° C, under a photoperiod of 12 and 16 hours of light and a light intensity of 30 μmol m -2 s -1 . At 21, 28, 35, 42 and 49 days after inoculation, the number of shoots per explant was evaluated. The treatment at the BAP concentration that gave the best multiplication rate (2.5 mg L -1 ) was set, and was tested in a further trial with different concentrations of NAA (0.0, 0.2, 0.4, 0 6, 0.8 or 1.0 mg L -1 ) under the same conditions as the previous experiment. The experimental designs were completely randomised, with five replications, and analysed in a 6 x 2 factorial. The data were submitted to analysis of variance and regression. No significant differences were seen in relation to the photoperiod or its interaction with the cytokinin and auxin under test. Multiplication was greater in the presence of 2.5 mg L -1 BAP, which gave a rate of 1.25 shoots/explant at 49 days of in vitro culture. The association of this BAP dosage with 1.0 mg L -1 NAA was even more efficient, producing 1.83 shoots per explant at 30 days of growth. The use of BAP together with NAA is beneficial to the induction of shoots in H. latispatha. Keywords: Heliconiaceae, 6-benzylaminopurine, naphthaleneacetic acid, tissue culture. RESUMO Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimentoConsiderando a crescente importância econômica das flores tropicais e as vantagens das técnicas aplicadas ao cultivo in vitro para estas plantas, torna-se necessária a realização de estudos que avaliem a propagação de espécies como a Heliconia latispatha. Diante do exposto, o objetivo do trabalho foi avaliar a indução de brotações in vitro em função de diferentes concentrações de BAP e ANA em função de fotoperíodos. Os explantes, oriundos de embriões zigóticos, foram inoculados em meio MS contendo diferentes concentrações de BAP (0,0; 0,5; 1,0; 1,5; 2,0 ou 2,5 mg L -1 ), mantendo-se as culturas em sala de crescimento com temperatura de 24,0 ± 2,0 ºC sob fotoperíodo de 12 e 16 horas de luz e intensidade luminosa de 30 μmol m -2 s -1 . Aos 21, 28, 35, 42 e 49 dias após a inoculação, foi avaliado o número de brotos obtidos por explante. O tratamento com a concentração de BAP que proporcionou melhor taxa de multiplicação (2,5 mg L -1 ) foi fixado e, em outro ensaio foi testado com diferentes concentrações de ANA (0,0; 0,2; 0,4; 0,6; 0,8 ou 1,0 mg L -1 ) nas mesmas condições do experimento ...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Rodrigues

Santos

Carvalho

2016

View full text Add to dashboard Cite

show abstract

“…Among the numerous culture media, the most used is that of Murashige and Skoog, known worldwide as MS media (Yadav and Singh, 2010). The main limiting factors for tissue culture production are the determination of the appropriate standards for growth regulators (Quisen and Angelo, 2008;Colombo et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

Lima

Silva

Cruz

et al. 2020

JAS

View full text Add to dashboard Cite

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

show abstract

Multiplicação de bastão-do-imperador em resposta a concentrações de BAP e número de subcultivos

Santos

Rodrigues

Silva

et al. 2016

View full text Add to dashboard Cite

The large ornamental potential of tropical flowers has stimulated the commercial cultivation of various species. Micropropagation is a viable alternate method of propagation, since it enables obtaining a higher number of seedlings with uniformity and pathogens free. The objective was to evaluate the in vitro multiplication rate of Etlingera elatior cv. Porcelana, using explants obtained from in vitro established seedling shoots, obtained from the 2nd subcultive. The explants were inoculated in MS culture medium containing different concentrations of BAP (0.0; 2.22; 4.44; 6.66; 8.88; and 11,10 μM), and the cultures maintained in a growth room with temperature 25.0 ± 2.0 °C under a photoperiod of 12 hours of light and light intensity of 30 μmol.m-2 s-1. The multiplication rate was monthly, according to the four subcultives, totaling 120 days. The experimental design was completely randomized, with four replications, analyzed in a factorial 4 x 6. The data were submitted to analysis of variance and regression. There were significant differences in subcultives and made for BAP concentrations used. For the first subcultive, the concentration of 2.22 μM of BAP afforded a rate of 4.06 sprouts per explant, already in the second and fourth subcultives, with the addition of cytokinin concentration was increased amount of sprouts reaching at a rate of 4.05 and 4.96 shoots/explant in the highest concentration of BAP. The results of the treatments evaluated indicate that the presence of BAP favored sprout emission. The concentrations of 2.22, 8.88 and 11.10 μM this cytokinin promoted the highest multiplication rates in the first, second and fourth subcultives, respectively.

show abstract

Estabelecimento de protocolo para multiplicação in vitro de Bastão-do-imperador (Etlingera elatior) Jack RM Sm.

Cited by 5 publications

References 17 publications

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Indução de brotações in vitro de Heliconia latispatha em função do fotoperíodo e reguladores de crescimento

Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

Multiplicação de bastão-do-imperador em resposta a concentrações de BAP e número de subcultivos

Contact Info

Product

Resources

About