Effect of phytase and protease combination on performance, metabolizable energy, and amino acid digestibility of broilers fed nutrient-restricted diets

Abstract

Three experiments were carried out to evaluate the effect of dietary combination of different enzymes (phytase + protease) on performance, metabolizable energy, and amino acid digestibility of broiler chickens fed diets with nutritional reduction. A total of 1,400, 336, and 384 male chickens were distributed in a completely randomized design, in the experiments of performance, metabolism, and digestibility, respectively. Treatments were divided as follows: positive control (PC), negative control -NC1 (PC minus 0.16% Ca, 0.15% available P (aP), and 0.5% crude protein (CP)), NC2 (PC minus 0.16% Ca, 0.15% aP, and 1% CP), NC1 added with phytase deriving from citrobacter and protease deriving from Bacillus licheniformis (CBE), NC1 added with phytase deriving from E. coli and protease deriving from microbial fermentation (SE), NC2 added with CBE, and NC2 added with SE. A protein-free diet was included in the digestibility experiment. The nutritional restriction did not affect feed intake of birds in the first experiment; however, the restriction inhibited body weight gain and feed conversion ratio in all phases. In experiment 2, the nutritional restriction decreased AME and AMEn values, although the addition of phytase and protease in diets improved both parameters, mainly in NC2. Treatment NC2 impaired the standardized digestibility of total essential amino acids of animals subjected to experiment 3, although the addition of enzymes helped to recover the digestibility to levels similar to PC. Supplementation of phytase in association with protease is effective to improve performance, energy metabolism, and standardized amino acid digestibility of broilers fed diets with nutrient restriction.

Evaluation of dietary hemp seed meal as soybean meal substitution on productive performance, egg quality, and yolk fatty acid composition of laying hens

“…Antinutrients cause physiological and functional effects in animals when consumed above tolerance levels. For instance, phytic acid can decrease protein digestibility and improve amino acids, endogenous nitrogen, and bivalent cation minerals excretion(Bernardes et al 2022). Cyanogenic glycoside levels exceeding 10 mg/kg are hazardous to chicken health (EFSA, 2019).…”

Isolation and characterization of a novel hydrolase-producing probiotic Bacillus licheniformis and its application in the fermentation of soybean meal

“…The collected supernatant was then diluted 10 times with PBS. Samples were centrifuged for 2 min at 400 × g and 100 μL of the original sample and 10-times diluted fecal suspension were plated on Luria-Bertani ( 19 ) agar plates and incubated at 37°C for 24 h. Thirty-seven bacterial colonies with different morphologies were purified using a streak plate method, and the purified bacteria were submitted to the Animal Health Laboratory at the University of Guelph for species verification using a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS, Bruker, Canada). Five isolates of B. licheniformis (tentatively named A4, A11, A16, B2, and B4) were selected for phytase analysis.…”

Avaliação de proteases exógenas em dietas para frangos de corte

“…Esses benefícios foram associados à uma melhor digestibilidade das proteínas da dieta devido a atuação das proteases em degradar rapidamente os fatores antinutricionais presentes na dieta, aumentando a disponibilidade de aminoácidos para a utilização pelo animal (Kamel et al, 2015). Bernardes et al (2022), trabalhando com a suplementação de fitase e protease para frangos de corte alimentados com dietas com redução de nutrientes, observaram melhoras nos parâmetros de desempenho como ganho de peso e conversão alimentar. Os efeitos da inclusão de protease exógena no desempenho de frangos de corte são mais evidenciados quando se utiliza dietas com redução de aminoácidos, proteína bruta ou mesmo quando há algum fator natural que compromete a digestibilidade da proteína (Amerah et al, 2017).…”

“…Estudos envolvendo a utilização de proteases exógenas em dietas para frangos de corte com redução de proteína bruta relataram benefícios em parâmetros de desempenho e também aumento na digestibilidade de nutrientes da ração (Stefanello et al, 2016;Cowieson et al, 2018;Bernardes et al, 2022). Entretanto, outros autores não observaram diferenças na digestibilidade de proteína bruta ou aminoácidos ileais (Erdaw et al, 2017;Borda-Molina et al, 2019).…”