Chitosan Nanoparticles Loaded with Carvacrol and Carvacryl Acetate for Improved Anthelmintic Activity

André, Weibson Paz Pinheiro; Paiva, José Ribamar; Cavalcante, Géssica Soares; Ribeiro, Wesley Lyeverton Correia; Filho, José Araújo; Cavalcanti, Bruno C.; Morais, Selene de; Oliveira, Lorena de; Beviláqua, Cláudia Maria Leal; Abreu, Flávia Oliveira Monteiro da Silva

doi:10.21577/0103-5053.20200047

Cited by 5 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dubey; J. Bajpai and A.K. Bajpai (2016), produced chitosan particles for the adsorption of Hg(II) ions with an average hydrodynamic dimension of 303 and 461 nm [ 42 ]. Filho et al (2019), when working with polysaccharide nanoparticles based on chitosan, alginate, and Arabic gum for encapsulation of anacardic acid and cardol, obtained most nanostructures in both systems, a high fraction of particles smaller than 250 nm [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Chitosan-Based Nanoparticles for Cardanol-Sustained Delivery System

et al. 2022

Self Cite

View full text Add to dashboard Cite

Cardanol, principal constituent of the technical cashew nut shell liquid, has applications as antioxidant and antibacterial, and these properties may be enhanced through encapsulation. In the present study, we isolated and purified cardanol, and nanoparticles (NPs) were produced by polyelectrolyte complexation using polysaccharide systems with chitosan, sodium alginate, and non-toxic Arabic gum, because they are biocompatible, biodegradable, and stable. We characterized the NPs for morphological, physicochemical, and antioxidant activity. The micrographs obtained revealed spherical and nanometric morphology, with 70% of the distribution ranging from 34 to 300 nm, presenting a bimodal distribution. The study of the spectra in the infrared region suggested the existence of physicochemical interactions and cross-links between the biopolymers involved in the encapsulated NPs. Furthermore, the NPs showed better antioxidant potential when compared to pure cardanol. Thus, the encapsulation of cardanol may be an effective method to maintain its properties, promote better protection of the active ingredient, minimize side effects, and can target its activities in specific locations, by inhibiting free radicals in various sectors such as pharmaceutical, nutraceutical, and biomedical.

show abstract

Section: Resultsmentioning

confidence: 99%

Chitosan-Based Nanoparticles for Cardanol-Sustained Delivery System

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…This phenomenon may be indicative that high pressurization with an ultrahomogenizer increases the effectiveness of carvone. The use of monoterpenes to inhibit the motility of adult nematodes has been previously reported (André et al ., 2016; Araújo-Filho et al ., 2018; André et al ., 2020). The monoterpenes citronellal and thymol used in the free form completely inhibit the motility of adult H. contortus after 12 and 24 h of exposure, respectively (André et al ., 2017; Araújo-Filho et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

In vitro anthelmintic activity of an R-carvone nanoemulsions towards multiresistant Haemonchus contortus

et al. 2022

View full text Add to dashboard Cite

This work aimed to evaluate the in vitro anthelmintic effect of carvone nanoemulsions on Haemonchus contortus. Three R-carvone nanoemulsions were prepared: uncoated R-carvone nanoemulsions homogenized in a sonicator (UNAlg-son) and homogenized in an ultrahomogenizer (UNAlg-ultra) and sodium alginate-coated R-carvone (CNAlg-ultra). The physicochemical characterizations of the nanoemulsions were carried out. The anthelmintic activity was evaluated using egg hatch test (EHT), larval development test (LDT) and adult worm motility test (AWMT). Changes in cuticle induced in adult H. contortus were evaluated by scanning electron microscopy (SEM). The results were subjected to analysis of variance and compared using the Tukey test (P < 0.05). The effective concentration to inhibit 50% (EC50) of egg hatching and larval development was calculated. The particle sizes were 281.1 nm (UNAlg-son), 152.7 nm (UNAlg-ultra) and 557.8 nm (CNAlg-ultra), and the zeta potentials were −15 mV (UNAlg-son), −10.8 mV (UNAlg-ultra) and −24.2 mV (CNAlg-ultra). The encapsulation efficiency was 99.84 ± 0.01%. SEM of the nanoemulsions showed an increase in size. In EHT, the EC50 values of UNAlg-son, UNAlg-ultra and CNAlg-ultra were 0.19, 0.02 and 0.17 mg mL−1, respectively. In LDT, they were 0.29, 0.31 and 0.95 mg mL−1 for UNAlg-son, UNAlg-ultra and CNAlg-ultra, respectively. The adult motility inhibition was 100% after 12 h of exposure to UNAlg-ultra and CNAlg-ultra, while for UNAlg-son, it was 79.16%. SEM showed changes in the buccal capsule and cuticular damage. It was concluded that R-carvone nanoemulsions showed antiparasitic action demonstrating promise for the control of infections caused by gastrointestinal nematodes in small ruminants.

show abstract

“…Nano encapsulated carvacryl acetate (nCVA) had no cytotoxic and genotoxic/mutagenic effects on murine fibroblast cell lines at the tested concentration. The non-toxic effects of nanoparticles were attributed to CVA and the biopolymers as they had no toxicity ( 24 ). Esophageal gavage administration of nanoencapsulated EcEO was safe, and no behavioral changes and mortality were recorded after acute toxicity evaluation ( 16 ).…”

Section: Discussionmentioning

confidence: 99%

Nanoparticles as Alternatives for the Control of Haemonchus contortus: A Systematic Approach to Unveil New Anti-haemonchiasis Agents

et al. 2021

View full text Add to dashboard Cite

Haemonchus contortus is an infectious gastrointestinal nematode parasite of small ruminants. This study addresses the in vitro/in vivo anti-haemonchiasis potential, toxicological effects, and mechanism of action of nanoparticles. Online databases were used to search and retrieve the published literature (2000 to 2021). A total of 18 articles were selected and reviewed, out of which, 13 (72.2%) studies reported in vitro, 9 (50.0%) in vivo, and 4 (22.2%) both in vitro/in vivo efficacy of different nanoparticles. Mostly, organic nanoparticles (77.7%) were used including polymeric (85.7%) and lipid nanoparticles (14.3%). The highest efficacy, in vitro, of 100% resulted from using encapsulated bromelain against eggs, larvae, and adult worm mortality at 4, 2, and 1 mg/ml, respectively. While in vivo, encapsulated Eucalyptus staigeriana oil reduced worm burden by 83.75% and encapsulated Cymbopogon citratus nano-emulsion by 83.1%. Encapsulated bromelain, encapsulated Eucalyptus staigeriana oil, and encapsulated Cymbopogon citratus nano-emulsion were safe and non-toxic in vivo. Encapsulated bromelain damaged the cuticle, caused paralysis, and death. Nanoparticles could be a potential source for developing novel anthelmintic drugs to overcome the emerging issue of anthelmintic resistance in H. contortus. Studies on molecular effects, toxicological consequences, and different pharmacological targets of nanoparticles are required in future research.

show abstract

Chitosan Nanoparticles Loaded with Carvacrol and Carvacryl Acetate for Improved Anthelmintic Activity

Cited by 5 publications

References 20 publications

Chitosan-Based Nanoparticles for Cardanol-Sustained Delivery System

Chitosan-Based Nanoparticles for Cardanol-Sustained Delivery System

In vitro anthelmintic activity of an R-carvone nanoemulsions towards multiresistant Haemonchus contortus

Nanoparticles as Alternatives for the Control of Haemonchus contortus: A Systematic Approach to Unveil New Anti-haemonchiasis Agents

Contact Info

Product

Resources

About