Effects of Light Quality and Chemical Elicitors on the Growth Parameters and Rosmarinic Acid Content of in vitro Cultures of Hyptis pectinata (L.) Poit.

Abstract

Hyptis pectinata (L.) Poit is a folk medicinal species in Northeast Brazil. In this work, seedlings of H. pectinata were cultured in vitro under diverse light quality spectra and under the influence of two distinct chemical elicitors, salicylic acid and silver nitrate (SN), at concentrations of 30 and 60 μM. Growth parameters, biomass and rosmarinic acid (RA) content were evaluated. RA may be associated with the functional properties of this plant. In the light quality experiments, the highest level of rosmarinic acid was found under red light (161.65 ± 8.64 μg g -1 DW (dry weight)) at 20 days (4.26-fold higher than the control white light). Among the evaluated elicitors, elicitation with 60 μM SN at 3 days and 30 μM SN at 6 days favored the highest rosmarinic acid content (6361.70 ± 216.96 and 4542.98 ± 92.53 μg g -1 DW, respectively), with relatively high biomass yield and a similar number of leaves and height as those of control seedlings. This approach is new for H. pectinata.

Effect of Light Conditions on Polyphenol Production in Transformed Shoot Culture of Salvia bulleyana Diels

“…However, this can be rectified by various methods, such as altering the light conditions. It has been shown that light (type of light source, light wavelength, its intensity, and exposure time) can be an important factor in stimulating plant growth, with some studies suggesting light to be one of the most important factors influencing the biosynthesis of bioactive compounds and, thus, the biological activity of raw materials [ 20 , 21 , 22 , 23 , 24 ]. Secondary metabolites, particularly polyphenols, together with antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), are scavengers of free radicals and can provide photoprotection against light stress.…”