Metabolite Fingerprinting and Profiling of the Medicinal Grass Eleusine indica Based on HPLC-DAD, UPLC-DAD-MS/MS and NMR Analyses

Peñaloza, Evelyn Maribel Condori; Casanova, Livia Marques; Leal, Ivana Corrêa Ramos; Aguiar, Paula Fernandes de; Costa, Sônia Soares

doi:10.21577/0103-5053.20180131

Cited by 9 publications

(15 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the flavonoids, schaftoside (C-glycosyl-6-C-arabinosyl-8 apigenin) and vitexin (apigenin-8-C-glucoside) have been isolated from aerial parts [14] . P-Coumaric acid and isoschaftoside have also been identified in the herbal medicine [26] . The compounds isolated from the organs of E. indica are summarized in Table 1: Aerial parts Lyophilized extract Isoschaftoside [26] Aerial parts Lyophilized extract acid p-coumarique [26] Aerial parts Lyophilized extract Vitexine [26] Aerial parts Hexane extract 1 -[[[(2-aminoethoxy) hydroxyphosphinyl]oxy]methyl]1-,2-ethandiylester [27] Aerial parts Methanol extract Acide hexadecanoique [27] Leave Essential oil Heptacosane [28] Leave Essential oil Pentatriacotene [28] Leave Essential oil Docosanol [28] Leave Essential oil 2-methylhexan-1-ol [28] Leave Essential oil Nonadecane [28] Leave Essential oil Cis-13-eicosenique [28] Leave Essential oil 2,6,10-trimethyltetradecane [28] Leave Essential oil Tert-hexadecanethiol [28] Leave Essential oil Nonadocane [28]…”

Section: Phytochemical Compositionmentioning

confidence: 99%

Review of the literature of Eleusine indica: phytochemical, toxicity, pharmacological and zootechnical studies

Adoho¹,

Zinsou²,

Olounladé³

et al. 2021

J Pharmacogn Phytochem

View full text Add to dashboard Cite

The objective of this study is to synthesize knowledge on the pharmacological properties, phytochemistry and toxicity of Eleusine indica. To achieve this, specific keywords were used to explore scientific databases such as PubMed, Google Scholar and Researchgate. The scientific data collected show that Eleusine indica has antidiabetic, antimalarial, antibacterial, antifungal, anti-trypanosomal, antihelmintic, antioxidant, anti-inflammatory, antiurolithiasic, antiviral, phytoremediating, hepatoprotective and phytoremediating properties. These pharmacological properties are associated with a richness of the herb in secondary metabolites. It has been reported, the presence of alkaloids, terpenes, flavonoids, tannins, anthraquinones, saponins, cardiac glycosides and anthracene glycosides in several extracts of the plant. In addition, schaftoside (C-glycosyl-6-C-arabinosyl-8 apigenin) and vitexin (apigenin-8-C-glucoside) were isolated from aerial parts. Toxicologically, an absence of toxicity was noted. All this makes Eleusine indica, a plant with an interesting pharmacological potential, which should be valorized by further work and the development of phytodrugs.

show abstract

Section: Phytochemical Compositionmentioning

confidence: 99%

Review of the literature of Eleusine indica: phytochemical, toxicity, pharmacological and zootechnical studies

Adoho¹,

Zinsou²,

Olounladé³

et al. 2021

J Pharmacogn Phytochem

View full text Add to dashboard Cite

show abstract

“…A study by Penaloza et al in 2018 using NMR spectroscopy reported that the aerial parts of E. indica contained four specific compounds-flavonoids schaftoside, isoschaftoside, vitexin, and p-coumaric acid. 9 They have also detected 29 unknown compounds which they grouped into fatty acid derivatives, amino acids, carbohydrates, and aromatics. 9 Among all these components, flavonoids, p-coumaric acid, and fatty acid derivatives have the potential to promote the inhibition of the P. aeruginosa quorum-sensing network.…”

Section: Quorum Sensing Inhibition Assay Against P Aeruginosamentioning

confidence: 99%

“…9 They have also detected 29 unknown compounds which they grouped into fatty acid derivatives, amino acids, carbohydrates, and aromatics. 9 Among all these components, flavonoids, p-coumaric acid, and fatty acid derivatives have the potential to promote the inhibition of the P. aeruginosa quorum-sensing network. A few plants, such as Centella asiatica L. and Psidium guajava L., which have been shown to inhibit the swarming motility of P. aeruginosa in a dose-dependent manner, have been reported to contain rich amounts of flavonoids, including kaempferol, quercetin, apigenin, rutin, and naringin.…”

Section: Quorum Sensing Inhibition Assay Against P Aeruginosamentioning

confidence: 99%

“…A study by Penaloza et al in 2018, which utilized mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy, found that the aerial parts of E. indica contain fatty acid derivatives and p-coumaric acid. 9 Several of these fatty acids have demonstrated activity against the swarming motility of P. aeruginosa in a study by Inoue et al in 2008, while p-coumaric acid has demonstrated activity against Agrobacterium tumefaciens NTL4, Chromobacterium violaceum 5999, Pseudomonas chlororaphis in a study by Bodini et al on soil bacteria in 2009. 10,11 To date, there has been no study on the efficacy of E. indica in combating bacterial infections through quorum sensing inhibition.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Quorum Quenching Activity of Eleusine indica Crude Ethanolic Extract against Pseudomonas aeruginosa and Serratia marcescens

Barcena¹,

Baldovino²,

Bañez³

et al. 2021

Acta Med Philipp

View full text Add to dashboard Cite

Introduction. Nosocomial contaminants such as Pseudomonas aeruginosa and Serratia marcescens are increasingly developing resistance to many antibiotics. One of the promising alternatives that may complement, if not substitute, the use of antibiotics is quorum quenching, the process of interfering with chemical signals that mediate communication between microorganisms. Eleusine indica, a ubiquitous grass used traditionally to treat infections, has been shown to contain metabolites, such as fatty acid derivatives and p-coumaric acid, capable of quorum quenching. To date, there has been no study on the quorum quenching activity of E. indica.Objectives. This study aimed to determine the in vitro activity of crude ethanolic extract of E. indica leaves against selected quorum-sensing regulated virulence factors of P. aeruginosa and S. marcescens.Methodology. E. indica leaves were collected, washed, air-dried, and homogenized. Following ethanolic extraction and rotary evaporation, the extract was screened for antimicrobial activity through disk diffusion test and broth microdilution assay. The quorum quenching activity of the extract against P. aeruginosa was measured through swarming motility assay, while the activity against S. marcescens was measured through swarming motility and pigment inhibition assays. The quorum quenching assays were conducted in triplicates, and analysis of variance (ANOVA) was performed to identify differences among the treatment groups.Results. Disk diffusion test revealed that no zones of inhibition formed against both P. aeruginosa and S. marcescens for varying concentrations of up to 200 mg/mL of the crude extract. Likewise, the MIC of the extract against both P. aeruginosa and S. marcescens was determined to be >200 mg/mL. However, it was shown that the extract, at 50 mg/mL, has statistically significant activity (p<0.05) against the swarming motility of P. aeruginosa, and it is 71.6% as effective in reducing the swarming area of the bacteria compared to cinnamaldehyde. This was not observed when the extract was tested against the swarming motility of and pigment production by S. marcescens. Conclusion.In this study, the quorum quenching activity of the crude ethanolic extract of E. indica leaves was found to be effective against P. aeruginosa but not against S. marcescens. The compounds that will be identified by further studies may conceivably be used as an adjunct therapy in P. aeruginosa infections and as coating agents in medical devices.

show abstract

“…The antidiabetic activity of ethanolic leaf extract of E. indica was investigated in a model of alloxan-induced diabetes in rats which showed that there are reduced blood glucose levels [ 26 ]. On the other hand, in Brazil, aqueous preparations of the grass E. indica are used for treating malaria and lung infections [ 27 ]. In Malaysia, E. indica is traditionally used in ailments associated with the liver and kidneys [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Potential Health Risks of Macro- and Microelements in Commercial Medicinal Plants Used to Treatment of Diabetes

Souza¹,

Melo²,

Nascimento³

et al. 2021

BioMed Research International

View full text Add to dashboard Cite

Information on the content of medicinal plants used in the treatment of diabetes is scarce in the literature. The objectives of this study were to determine the levels of macroelements and microelements in three different medicinal plant species including the dry samples and teas from Bauhinia forficata, Eleusine Indica, and Orthosiphon stamineus and assess the human health risks of ingestion of the tea. The content of the dry samples and teas was obtained using the technique of inductively coupled plasma optical emission spectrometry (ICP OES) after microwave digestion procedure. The hazard quotient (HQ) method was used to access the human health risks posed by heavy metal through tea consumption. The results revealed the presence of K, Mg, Na, P, Al, Fe, Zn, Mn, Cu, Ni, and Se in dry samples and plant teas. The dry plants have high concentration of K and P. All dry plants contain Mg, Na, Al, Fe, Mn, Ni, Zn, and Cu above the limit permissible level set by the World Health Organization (WHO). All the hazard index (HI) values in plant teas were found to be within safe limits for human consumption ( HI < 1 ). The plants may have possible action benefits when used in popular medicine. However, the ingestion through capsules prepared by enclosing a plant powder or teas can be harmful to the health of diabetics. The prescription of this plant for the treatment of diabetes should be treated with caution.

show abstract

Metabolite Fingerprinting and Profiling of the Medicinal Grass Eleusine indica Based on HPLC-DAD, UPLC-DAD-MS/MS and NMR Analyses

Cited by 9 publications

References 11 publications

Review of the literature of Eleusine indica: phytochemical, toxicity, pharmacological and zootechnical studies

Review of the literature of Eleusine indica: phytochemical, toxicity, pharmacological and zootechnical studies

In Vitro Quorum Quenching Activity of Eleusine indica Crude Ethanolic Extract against Pseudomonas aeruginosa and Serratia marcescens

Potential Health Risks of Macro- and Microelements in Commercial Medicinal Plants Used to Treatment of Diabetes

Contact Info

Product

Resources

About