Simultaneous Determination of Four Antibiotics in Raw Milk by UPLC-MS/MS Using Protein Precipitation as Sample Preparation: Development, Validation, and Application in Real Samples

Magon, Thiago Ferreira dos Santos; Silveira, Roberta da; Galuch, Marília B.; Fagan, Eder Paulo; Feitoza, Ana Flavia D.; Palombini, Sylvio V.; Santos, Oscar Oliveira; Visentainer, Jesuı́ V.

doi:10.21577/0103-5053.20180121

Cited by 6 publications

(2 citation statements)

References 26 publications

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“…Cows metabolize tetracycline at a rate of 25 to 75%, and a sizable amount of the drug is excreted in cow milk [21]. Consumption of milk from the treated animals with tetracycline, is restricted to no more than 4 days after milk discharge [22].…”

Section: Resultsmentioning

confidence: 99%

The occurrence of doxycycline residue in raw dairy milk from Malang and Padang Panjang, Indonesia

Widiastuti

Martindah

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Antibiotic residue in milk has a negative impact on human health. The objective of this study was to determine the presence of doxycycline residues in fresh dairy milk and to evaluate its dietary intake to Indonesian population through milk consumption. Ninety milk samples were collected from the District of Malang (East Java Province) and sixty-six milk samples from Padang Panjang City (West Sumatera Province). All samples were analyzed for doxycycline residue using high-performance liquid chromatography (HPLC). The estimated daily intake (EDI) was determined by multiplying the mean concentration of antibiotics and the average daily milk consumption dividing it by the weight of population target. The occurrence of doxycycline residue obtained in raw milk samples was 36.67% for samples from Malang and 12.12% for samples from Padang Panjang. The range and average concentration for doxycycline residue in milk samples were 44.8-557.2 ng/g and 203.0 ng/g for samples from Malang and 14.2-64.5 ng/g and 30.1 ng/g for samples from Padang Panjang, respectively. Samples from Malang shows the highest EDI values of doxycycline both for adult, and children population and demonstrated the toxicological risk of consumption doxycycline together with the present other types or groups of antibiotics residue in milk or other types of foods. The results obtained show that, to prevent the negative effects of antibiotic residues on human health, the presence of antibiotic residues in raw dairy milk still needs to be monitored regularly.

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Section: Resultsmentioning

confidence: 99%

The occurrence of doxycycline residue in raw dairy milk from Malang and Padang Panjang, Indonesia

Widiastuti

Martindah

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…In the case of mastitis treatment, older cows were slower to eliminate tetracycline [ 26 ]. Therefore, consuming milk from an animal treated with tetracycline and developing subclinical mastitis should be restricted to at least 4 days after milk discharge [ 27 ]. High dosages and disregard for grace periods while using antibiotics in dairy production could endanger public health and cause financial loss for the dairy industry, because antibiotics are stable during pasteurization – only 5.74% and 15.3% of tetracycline and oxytetracycline decreased, respectively [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Tetracycline residues in fresh dairy milk from three districts in Indonesia: Occurrence and dietary exposure assessment

Widiastuti,

Martindah,

Anastasia

2023

Vet World

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Background and Aim: Milk can introduce antibiotics into the human diet which poses a public health risk. Therefore, a study to determine the tetracycline residue in dairy milk and its health risk assessment is needed. A cross-sectional study was performed to detect tetracycline residues in fresh dairy milk samples collected from the districts of Malang, Boyolali, and Padang Panjang, Indonesia, and to evaluate dietary exposure to tetracycline residues through milk consumption in 10-12-year-old children and adults. Materials and Methods: A total of 203 fresh dairy milk samples were collected from local and smallholder dairy cows in Malang, Boyolali, and Padang Panjang in April and August 2018. High-performance liquid chromatography equipped with a photodiode array at 355 and 368 nm was used to detect tetracycline residues. Data were evaluated for dietary exposure assessment. Results: The results showed that the most common residue found was chlortetracycline (8.37%), followed by tetracycline (7.88%) and oxytetracycline (5.91%) in the concentration range of 14.8-498.4, 11.7-49.4, and 11.6-85.6 ng/g, respectively. Seven (3.45%) samples exceeded the maximum residue limit (MRL) for chlortetracycline. However, neither oxytetracycline nor tetracycline residues exceeded the MRL. The mean concentration of the tetracycline residues was 21.76-137.05 ng/g, resulting in an estimated daily intake of 16.46-172.83 ng/kg body weight/day. Conclusion: Tetracycline residues were found in almost all milk sampling locations. The highest prevalence and residue concentration were obtained from chlortetracycline. Estimated daily intake of tetracycline through milk by 10–12-year-old children and adult consumers was low and the risk to consumers was negligible. Keywords: dietary exposure assessment, fresh dairy milk, high-performance liquid chromatography, residues, tetracycline.

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Validation of UHPLC-MS/MS Method and Measurement Uncertainty Evaluation for Lactose Quantification in Lactose-Free and Regular UHT Milk

Chiavelli

Galuch²,

Senes

et al. 2022

Food Anal. Methods

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Simultaneous Determination of Four Antibiotics in Raw Milk by UPLC-MS/MS Using Protein Precipitation as Sample Preparation: Development, Validation, and Application in Real Samples

Cited by 6 publications

References 26 publications

The occurrence of doxycycline residue in raw dairy milk from Malang and Padang Panjang, Indonesia

The occurrence of doxycycline residue in raw dairy milk from Malang and Padang Panjang, Indonesia

Tetracycline residues in fresh dairy milk from three districts in Indonesia: Occurrence and dietary exposure assessment

Validation of UHPLC-MS/MS Method and Measurement Uncertainty Evaluation for Lactose Quantification in Lactose-Free and Regular UHT Milk

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