Determinação De Designer Drugs Em Saliva Por Paper Spray Mass Spectrometry

Abstract

Recebido em 23/01/2019; aceito em 06/05/2019; publicado na web em 27/06/2019 DETERMINATION OF DESIGNER DRUGS IN SALIVA BY PAPER SPRAY MASS SPECTROMETRY. Determination of two drugs of abuse in saliva has been performed by Paper Spray Ionization Mass Spectrometry (PSI-MS) using a Molecularly Imprinted Polymer (MIP) coated paper as substrate. The method has been optimized for quantification of 3,4-methylenedioxy-N-methylamphetamine (MDMA) and methamphetamine, 3,4-methylenedioxyamphetamine (MDA) from synthetic saliva samples. Rapid analysis (~ 1 min) of designer drugs in saliva is demonstrated using MIP-PSI-MS. Both precursor ions of MDMA and MDA were confirmed by tandem mass spectrometry (MS/MS) experiments by elucidating the fragmentation patterns formed via collisioninduced dissociation (CID). The MIP-PSI-MS method showed greater selectivity for MDMA and MDA in comparison with other analytes. Signal intensity ratios of the most important transitions for MDMA (194 → 163) and MDA (180 → 163) were found linear (R > 0,99) in the range 25-500 µg L-1. The LOD and LOQ for MDMA were 1.11 µg L-1 and 18.73 µg L-1 , respectively, and for MDA were 3.42 µg L-1 and 21.66 µg L-1 , respectively. The precision and accuracy values were determined below 4%, and the recoveries about 100%, without pre-concentration/enrichment or separation steps. The MIP-PSI-MS method displays the potential for rapid screening for selective quantitative determination of illicit drugs in saliva samples.

Multivariate classification techniques and mass spectrometry as a tool in the screening of patients with fibromyalgia

“…Thus, it is necessary to develop analytical methods that prioritize the resolution of these problems. With Ambient Mass Spectrometry (AMS) 8 , and the introduction of techniques such as: direct Analysis in Real Time (DART) and Desorption Electrospray Ionization (DESI), there is a new way to generate ions in MS 7 , 9 . AMS is characterized by ion generation under ambient conditions and direct compound analysis in its native environment with minimal or no sample preparation 10 .…”

Detecção de esteroides anabólicos androgênicos e seus produtos de biotransformação em dried urine spots por ionização por paper spray acoplada à espectrometria de massas (PS-MS)

“…A técnica de ionização por paper spray vem sendo aplicada com sucesso em análises rápidas em diversas matrizes biológicas como urina, sangue total, saliva, tecidos, dried blood spots e dried urine spots (MANICKE et al, 2011;WANG et al, 2011;ZHANG et al, 2012;YANG et al, 2012;SU et al, 2013;KLAMPFL & HIMMELSBACH, 2015;VEGA et al, 2016;MAURER, 2017c;YANNELL et al, 2017;KEATING et al, 2018;MACIEL et al, 2019). O PS-MS já foi utilizado em análises de fármacos terapêuticos e seus respectivos produtos de biotransformação em DBS, na qual os analitos foram extraídos da matriz pelo solvente de pulverização (ZHANG et al, 2012;OUYANG, 2018).…”

Paper Spray Ionization Mass Spectrometry as a Potential Tool for Early Diagnosis of Cervical Cancer

“…When a high voltage is applied to the paper, a spray with charged droplets is generated at the paper tip and goes toward the mass spectrometer inlet for MS analysis . The analytical performance of PSI-MS has been evaluated in a wide range of applications, including the detection of drugs in fingerprint samples, quantifications of pesticides in fruits and vegetables, , characterization of energetic polymers, investigations of inks and questioned documents, analysis of drugs of abuse, , and coupling to portable mass spectrometers for on-site analysis . In addition, PSI-MS has been widely employed in clinical diagnosis, − because of its ability to allow biofluid analysis using a small amount of sample with minimal or even no sample preparation (for example, by applying few microliters of plasma, serum, or whole blood directly onto a triangular paper), which can significantly reduce the invasiveness to the patients and waiting time for clinical reports.…”