Fourier Transform Infrared Spectroscopy, Thermogravimetric Analysis, Scanning Electron Microscopy as Supporting Tools in Quality Control of Antiparasitics

Silva, Michelli dos Santos; Gonring, Karla Loriatto; Silva, Ricky Cássio Santos da; Fonseca, Matheus Cecílio; Borges, Marcella Matos Cordeiro; Nunes, Otalíbio C.; Forim, Moacir Rossi; Borges, Keyller Bastos; Borges, Warley de Souza

doi:10.21577/0100-4042.20170168

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…3 The chemical structures of fluazuron and clorfluazuron (internal standard) are given in Figure 1. Despite the lack of an official method for quantification of fluazuron, some authors have reported methods for quantification of the drug in different matrices: tissues, 4-6 serum, 7 tea, 8 vegetables 9 and pharmaceutical formulations, 10 involving liquid chromatography (LC) combined with ultraviolet detection (UV), Fourier-transform infrared spectroscopy (FTIR) and/or tandem mass (MS/MS) detection. In these methods, the sample preparation is by solid-liquid extraction, 6,9 ultrasound assisted solid-liquid extraction, 4,8 and salting out solid-liquid extraction.…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical Method to Measure Fluazuron in Bovine Plasma and its Application in Pharmacokinetic Studies

Ferreira¹,

Lima²,

Magalhães³

et al. 2019

Rev. Virtual Quim

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Método Bioanalítico para o Fluazuron em Plasma Bovino e Sua Aplicação em Estudos de FarmacocinéticaResumo: Fluazuron é um regulador de crescimento de insetos pertencente à classe da benzofeniluréia que é comercializado para o controle do carrapato bovino. Embora os reguladores de crescimento sejam uma classe amplamente estudada, o número de artigos descrevendo métodos analíticos para determinar o fluazuron em amostras de plasma animal é reduzido. Para avaliar a característica farmacocinética do fluazuron em bovinos, foi avaliado e validado um método CL-UV. As condições experimentais foram otimizadas pelo estudo comparativo de três métodos de extração: ELL, EFS e DMFS. A separação cromatográfica foi realizada na coluna Kromasil C18, com fase móvel de acetonitrila: água (80:20, v / v) na vazão de 1,0 mL/min-1. O método foi validado de acordo com as exigências da ANVISA dentro da faixa de 20 a 150 ng mL-1 (r > 0,99). O LD e LIQ para o fluazuron foram 10 e 20 ng mL -1 no plasma. A precisão e exatidão inter / intra-ensaio foram ≤ 9,48 % (CV) e ≤ 10,3 % (EPR), respectivamente. Como resultado, foram obtidos parâmetros farmacocinéticos de C max (74,4 ± 3,52 ng mL -1 ), AUC 0-t (1007 ± 33,5 ng*d mL -1 ) e t 1/ 2 (14,6 ± 2,97 d) para fluazuron após administração tópica em bovinos. O método bioanalítico foi adequado para a aplicação nos estudos de farmacocinética, uma vez que os valores de LIQ para o fluazuron permitiram que a quantificação atingisse a AUC 0-t superior a 80 % da AUC 0-∞.

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Section: Introductionmentioning

confidence: 99%

Bioanalytical Method to Measure Fluazuron in Bovine Plasma and its Application in Pharmacokinetic Studies

Ferreira¹,

Lima²,

Magalhães³

et al. 2019

Rev. Virtual Quim

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“…As shown in Figure 5 nTBZ absorption bands are confined from 3,100-430 cm −1 region, most of the peaks are displayed in the fingerprint region (1,500-400 cm −1 ) demonstrate the complexity of the molecule. The FTIR spectrum of nTBZ revealed the presence of the C-H stretching of the benzene and the thiazole rings between 3,100 and 3,000 cm −1 (Silva et al, 2018). The bands around 2,980 cm −1 characterize tertiary amines C-N=C of the imidazole and the thiazole rings.…”

Section: Ftir Analysismentioning

confidence: 98%

Tunisian hypersaline sediments to set up suitable halotolerant microbial bioanodes for electrostimulated biodegradation of thiabendazole

et al. 2022

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This study investigated for the first time the performance of microbial halotolerant bioanodes designed from two Tunisian Hypersaline Sediments (THS) for simultaneous electrostimulated biodegradation of synthetic fruit packaging wastewater containing thiabendazole (TBZ), and recovery of an anodic current signal. Halotolerant bioanodes formation has been conducted on 6 cm2 carbon felt electrodes polarized at −0.1Vvs Saturated Calomel Electrode (SCE), inoculated with 80% (v:v) of synthetic wastewater containing 50 ppm of irradiated or not irradiated TBZ and 20% (v:v) of THS for a period of 7 days. Microbial bioanodes, and the corresponding anolytes, i.e., synthetic wastewater, were studied comparatively by electrochemical, microscopic, spectroscopic, molecular and microbial ecology tools. Despite the low maximum current densities recorded in the 50 ppm TBZ runs (3.66 mA/m2), more than 80% of the TBZ was degraded when non-irradiated TBZ (nTBZ) was used as the sole carbon energy by the microorganisms. Nevertheless, the degradation in the presence of irradiated TBZ (iTBZ) was greatly reduced by increasing the irradiation dose with maximum current density of 0.95 mA/m2 and a degradation rate less than 50% of iTBZ. In addition, chemical changes were observed in TBZ as a result of gamma irradiation and bioelectrochemical degradation. FT-IR and UV-Vis techniques confirmed the degradation of TBZ structural bonds producing novel functional groups. Culture-dependent approach and 16S ribosomal RNA sequencing demonstrated that bacterial community of halotolerant bioanodes formed with nTBZ were dominated by Proteobacteria (75%) and Firmicutes (25%). At species level, enrichment of Halomonas smyrnensis, Halomonas halophila, Halomonas salina, Halomonasor ganivorans and Halomonas koreensis on carbon felt electrodes were correlated with maximal current production and nTBZ degradation. As a result, THS halotolerant bacteria, and specifically those from Chott El Djerid (CJ) site certainly have well established application for the electrostimulated microbial biodegradation of fungicide in the real fruit and vegetable processing industries.

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“…The terahertz spectrum of thiabendazole, at room temperature, has also been reported and assigned [ 13 ]. Santos Silva et al [ 14 ] investigated TBZ (together with a few other compounds which exhibit anti-parasitic bioactivity) by infrared spectroscopy and thermogravimetry. The authors focused on analytical issues and investigated the stability of the studied compounds under different conditions.…”

Section: Introductionmentioning

confidence: 99%

Thiabendazole and Thiabendazole-Formic Acid Solvate: A Computational, Crystallographic, Spectroscopic and Thermal Study

et al. 2020

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Thiabendazole (TBZ) is a substance which has been receiving multiple important applications in several domains, from medicine and pharmaceutical sciences, to agriculture and food industry. Here, a comprehensive multi-technique investigation on the molecular and crystal properties of TBZ is reported. In addition, a new solvate of the compound is described and characterized structurally, vibrationally and thermochemically for the first time. Density functional theory (DFT) calculations were used to investigate the conformational space of thiabendazole (TBZ), revealing the existence of two conformers, the most stable planar trans form and a double-degenerated-by-symmetry gauche form, which is ~30 kJ mol−1 higher in energy than the trans conformer. The intramolecular interactions playing the major roles in determining the structure of the TBZ molecule and its conformational preferences were characterized. The UV-visible and infrared spectra of the isolated molecule (most stable trans conformer) were also calculated, and their assignment undertaken. The information obtained for the isolated molecule provided a strong basis for the understanding of the intermolecular interactions and properties of the crystalline compound. In particular, the infrared spectrum for the isolated molecule was compared with that of crystalline TBZ and the differences between the two spectra were interpreted in terms of the major intermolecular interactions existing in the solid state. The analysis of the infrared spectral data was complemented with vibrational results of up-to-date fully-periodic DFT calculations and Raman spectroscopic studies. The thermal behavior of TBZ was also investigated using differential scanning calorimetry (DSC) and thermogravimetry. Furthermore, a new TBZ–formic acid solvate [2-(1,3-thiazol-4-yl)benzimidazolium formate formic acid solvate] was synthesized and its crystal structure determined by X-ray diffraction. The Hirshfeld method was used to explore the intermolecular interactions in the crystal of the new TBZ solvate, comparing them with those present in the neat TBZ crystal. Raman spectroscopy and DSC studies were also carried out on the solvate to further characterize this species and investigate its temperature-induced desolvation.

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Fourier Transform Infrared Spectroscopy, Thermogravimetric Analysis, Scanning Electron Microscopy as Supporting Tools in Quality Control of Antiparasitics

Cited by 5 publications

References 23 publications

Bioanalytical Method to Measure Fluazuron in Bovine Plasma and its Application in Pharmacokinetic Studies

Bioanalytical Method to Measure Fluazuron in Bovine Plasma and its Application in Pharmacokinetic Studies

Tunisian hypersaline sediments to set up suitable halotolerant microbial bioanodes for electrostimulated biodegradation of thiabendazole

Thiabendazole and Thiabendazole-Formic Acid Solvate: A Computational, Crystallographic, Spectroscopic and Thermal Study

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