Mean Platelet Volume: is it an Emerging Marker or an Exaggeration?

Kaya, Uğur; Koza, Yavuzer; Çolak, Abdürrahim

doi:10.21470/1678-9741-2018-0425

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…Platelets are known to be very sensitive to manipulation in vitro and can be easily activated during preparation processes, such as centrifugation, labeling, and changes in temperature and pressure. Prior studies have documented that the MPV of the platelet increases as the time interval between blood collection and analysis increases; thus, the MPV should be determined within 1 h of collection to avoid interference caused by external storage time, 32 indicating that point-of-care property needed in clinical platelet testing. In addition, the high cost hindered the practical use of expensive equipment in underdeveloped regions where costly assays were not feasible.…”

Section: ■ Introductionmentioning

confidence: 99%

Rapid and Nondestructive Evaluation of Platelet Function in Whole Blood by Microfluidic Deterministic Cytometry

Chen,

Deng,

Zhang

et al. 2023

Anal. Chem.

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Platelet size is a determinant of platelet function.Here, a new microfluidic deterministic cytometry packed with Sshaped micropillars (S-MDC) was developed to rapidly and sensitively determine the apparent size (D app ) of platelets, which was used to evaluate platelet function. The platelet D app in the diluted whole blood was rapidly and label-freely measured by S-MDC within 2 min under shear rates (0.4 mm/s) that mimicked physiological conditions. The level of CD62p on platelets scarcely changed before and after platelets went through the whole S-MDC, indicating that the platelet function was nondestructive. Notably, the human platelet D app determined before and after thrombin addition by S-MDC was highly coincident with the levels of CD62p on the platelet surface by flow cytometry (r = 0.819), revealing that the human platelet D app was available to assess the platelet activation state. In addition, the results of the rat platelet D app were consistent with myocardial injury of rats with myocardial ischemia before and after treatment with antiplatelet agents, suggesting that rat platelet D app can be used to reflect myocardial injury in vivo outcomes. These findings reveal that S-MDC is a promising technique for screening tests for a bleeding disorder, in addition to monitoring antiplatelet drugs.

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Section: ■ Introductionmentioning

confidence: 99%

Rapid and Nondestructive Evaluation of Platelet Function in Whole Blood by Microfluidic Deterministic Cytometry

Chen,

Deng,

Zhang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…2,3 Also, the time elapsed from venipuncture to MPV measurement can affect its results. 4,5 Yet, standardization of MPV measurement needs large scale studies with sufficient power. 6 Meanwhile, we used a routine measurement approach like most of previous studies.…”

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confidence: 99%