Acetaminophen Mitigates Myocardial Injury Induced by Lower Extremity Ischemia-Reperfusion in Rat Model

Abstract

ObjectiveThe injury-reducing effect of acetaminophen, an effective analgesic and antipyretic on ischemia-reperfusion continues to attract great attention. This study analyzed the protective effect of acetaminophen on myocardial injury induced by ischemia-reperfusion in an experimental animal model from lower extremity ischemia-reperfusion.MethodsTwenty-four Sprague-Dawley female rats were randomized into three groups (n=8) as (i) control group (only laparotomy), (ii) aortic ischemia-reperfusion group (60 min of ischemia and 120 min of reperfusion) and (iii) ischemia-reperfusion + acetaminophen group (15 mg/kg/h intravenous acetaminophen infusion starting 15 minutes before the end of the ischemic period and lasting till the end of the reperfusion period). Sternotomy was performed in all groups at the end of the reperfusion period and the heart was removed for histopathological examination. The removed hearts were histopathologically investigated for myocytolysis, polymorphonuclear leukocyte (PMNL) infiltration, myofibrillar edema and focal hemorrhage.ResultsThe results of histopathological examination showed that acetaminophen was detected to particularly diminish focal hemorrhage and myofibrillar edema in the ischemia-reperfusion + acetaminophen group (P<0.001, P=0.011), while there were no effects on myocytolysis and PMNL infiltration between the groups (P=1.000, P=0.124).ConclusionAcetaminophen is considered to have cardioprotective effect in rats, by reducing myocardial injury induced by abdominal aortic ischemia-reperfusion.

Effects of alpha-lipoic acid on skeletal muscle ischemia-reperfusion injury in mice

“…The main mechanism of cardiovascular diseases, the most common cause of death worldwide, is ischemia-reperfusion injury [2]. For these reasons, ischemia reperfusion injury has been an area of interest for researchers [3,4].…”

Cardioprotective effects of sinomenine in myocardial ischemia/reperfusion injury in a rat model

“…Antioxidant substances are crucial in countering the damage caused by free radicals. It is widely known that during I/R injury, the antioxidant capability is suppressed, and an imbalance of oxidative/antioxidative molecules contributes to the oxidative balance in myocardial ischemia patients ( Geldi et al, 2018 , Li et al, 2019 ). The similar result was observed in the I/R group and sinomenine treated group exhibited the improved the antioxidant level and suppressed the production of free radicals.…”

“…I/R injury leads to the induction of arrhythmias, microvascular injury, myocardial dysfunction and “no-reflow” phenomenon ( Najafi et al 2018 ). Previous research has suggested that necrosis, autophagy, and apoptosis are important factors in inducing cell death during the reperfusion phase of I/R injury ( Geldi et al, 2018 , Qiao et al, 2019 ). Normally, the weakness in impulse conduction or dysfunction in the impulse generation occurs due to a lack of hypoxia and ATP that results in mitochondrial dysfunction, which is considered the main parameter for inducing ischemia induced arrhythmias ( Han et al, 2019 , Qiao et al, 2019 ).…”

“…Recent years have seen significant enhancements in the protective strategies to suppress all features of post ischemic injury in cardiovascular diseases ( Gatzke et al, 2018 , Liu et al, 2019 ). Due to the scarcity of therapy options, a safer and more effective technique for developing cardiovascular drugs is urgently needed ( Badalzadeh et al, 2014 , Geldi et al, 2018 ).…”

Effects of N-Acetylcysteine and N-Acetylcysteine Amide on Erythrocyte Deformability and Oxidative Stress in a Rat Model of Lower Extremity Ischemia-Reperfusion Injury

“…Many trials have been performed on various substances that may have a protective effect on lower extremity ischemia-reperfusion injury [ 3 ]. There are also many studies showing that reperfusion injury following lower extremity ischemia causes erythrocyte deformation [ 4 ].…”